| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,896,410 | A→G | 8.7% | intergenic (+43/‑165) | PP_4304 → / → sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,896,410 | 0 | A | G | 8.7% | 129.4 / 2.7 | 47 | intergenic (+43/‑165) | PP_4304/sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (18/24); new base G (2/2); total (20/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
GGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCC > NC_002947/4896264‑4896554 | gggAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAgg < 8:45234/149‑1 (MQ=255) gcagcaTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑t < 2:350372/149‑1 (MQ=255) agcaTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑Tgc > 8:42407/1‑149 (MQ=255) aTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGcgcg < 3:79903/149‑1 (MQ=255) aTTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTc < 1:290845/149‑1 (MQ=255) ttGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTcc < 8:6525/149‑1 (MQ=255) gCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTcc‑‑‑cc > 3:266672/1‑149 (MQ=255) gACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCg < 8:265313/147‑1 (MQ=255) gACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCg > 7:265313/1‑147 (MQ=255) gACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTa < 4:215834/149‑1 (MQ=255) gACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTa < 5:59328/149‑1 (MQ=255) cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCgg < 7:121595/149‑1 (MQ=255) cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCgg < 7:42407/149‑1 (MQ=255) cacaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCgg < 1:261079/149‑1 (MQ=255) agaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGttt < 4:266672/149‑1 (MQ=255) gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTa < 1:27519/149‑1 (MQ=255) gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGACGGCCTCTTCGCGGGGAAATCCGCTCCTACATGCGCCGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTa > 7:251213/1‑149 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGG‑‑‑GGACCG‑CGCACGGTCCGCGCCTGTAGGAGCGGATTTAc > 7:224421/1‑144 (MQ=14) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGG‑‑‑GGACCG‑CGCACGGTCCGCGCCTGTAGGAGCGGATTTAc < 8:224421/144‑1 (MQ=14) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCgg < 2:338991/138‑1 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCgg > 1:338991/1‑138 (MQ=255) aGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGG‑‑‑GGACCG‑CGCACGGTCCGCGCCTGTAGGAGCGGATTTACCCGCGAagag < 2:180019/149‑1 (MQ=14) gCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGGGGACCG‑CGCACGGTccgcgc < 6:33750/123‑5 (MQ=38) gCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGGGGACCG‑CGCACGGTccgcgc > 5:33750/1‑119 (MQ=38) cggcgTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCGCCTGTAGGAGCGGatttac > 2:234523/1‑98 (MQ=38) cggcgTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCGCCTGTAGGAGCGGatttac < 1:234523/103‑6 (MQ=38) ttctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTcc‑‑‑ccc < 2:347996/109‑1 (MQ=255) ttctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTcc‑‑‑ccc > 1:347996/1‑109 (MQ=255) ctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGa < 3:127042/148‑1 (MQ=255) tctcGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGAGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCa > 5:382631/1‑148 (MQ=255) tcGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGGAGGAGCCGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATaa > 5:173636/1‑149 (MQ=255) tttCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTAGCCGGTAAATACGCTCCTAAAGGCGCGGAACG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCCGGTTTACCCGCCAAGAGGCCGGCACAAGCAGAACCATAACCAAAGCAAttttttttat > 4:298714/1‑147 (MQ=255) ccAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTaa > 6:108749/1‑149 (MQ=255) tAGCGCCTGCGCCGGCCTATTCGCTGGTAAATCCGATCCTACTGGCGCGGACCG‑TGCGCGTTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATaaa < 8:251213/148‑1 (MQ=39) gcgcCTGCGCCGGCCTCTTCGCGGTTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTTGTAGGGGTTTTACCCGCTAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAacc < 1:378486/148‑1 (MQ=255) gcCGGCCTCTTCGCGGGTAAATCGGCGCCTACAGGCGCGGACCG‑TGTGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTta < 6:382631/149‑1 (MQ=255) cGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTtata > 6:218569/1‑149 (MQ=255) tctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGc > 7:124656/1‑149 (MQ=255) ctTCGCGGGTAAACCCGCTCCTACGGG‑‑‑GGACCG‑CGCACGGTCCGCGCCTGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCt > 8:226570/1‑149 (MQ=14) cgcgGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTgg > 7:149664/1‑148 (MQ=255) aaTCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGc < 5:108749/148‑1 (MQ=255) aaTCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CACGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGc < 3:298714/148‑1 (MQ=255) aTCCGCTCCTACAGGCGCGGACCGTTGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcc > 7:56886/1‑149 (MQ=255) gCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGt > 8:71173/1‑109 (MQ=255) gCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGt < 7:71173/109‑1 (MQ=255) cTACAGGCGCGGACCG‑TGGGCGGTCC‑‑‑CCCGTAGTAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACAccc < 6:173636/149‑1 (MQ=255) cTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACAccc > 1:110740/1‑149 (MQ=255) aGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTg < 2:328208/126‑1 (MQ=255) | GGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCG‑TGCGCGGTCC‑‑‑CCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCC > NC_002947/4896264‑4896554 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |