Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,899,256 C→A 5.1% A71A (GCG→GCT)  PP_4307 ← DMT superfamily permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,899,2560CA 5.1% 284.4 / 3.9 91A71A (GCG→GCT) PP_4307DMT superfamily permease
Reads supporting (aligned to +/- strand):  ref base C (42/43);  new base A (2/3);  total (45/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GATCTTTTCGCCCAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCGGACACCAGCACGAACCAGCCA  >  NC_002947/4899109‑4899403
                                                                                                                                                   |                                                                                                                                                   
caTCTTTTCGCCCAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCg                                                                                                                                                    <  2:343860/148‑1 (MQ=255)
     tttCGCCCAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAg                                                                                                                                                >  1:234568/1‑148 (MQ=255)
      ttCGCCCAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCa                                                                                                                                                 <  7:28265/146‑1 (MQ=255)
         gCCCAGAAACAGCGCCGCCAGCCCCACCAGCATTAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGTCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCGATAGCCAGcgggg                                                                                                                                           <  5:224640/149‑5 (MQ=255)
         gCCCAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCaaa                                                                                                                                            <  5:55602/148‑1 (MQ=255)
          cccAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTTTACACAGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCTAACAACAGCAGCCAGTTGCCGAAAATGTCAACCCCGCTGGCTATAGCCAGCaaaaa                                                                                                                                          >  2:307256/1‑149 (MQ=255)
          cccAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTCGCTATCGCCAGCaaaaa                                                                                                                                          >  2:218439/1‑149 (MQ=255)
           ccAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAAt                                                                                                                                         >  1:155536/1‑149 (MQ=255)
           ccAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAAt                                                                                                                                         >  7:213465/1‑149 (MQ=255)
           ccAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCGATAGCCAGcggggtg                                                                                                                                         <  2:72129/149‑7 (MQ=255)
             aGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGc                                                                                                                                       >  4:367921/1‑149 (MQ=255)
               aaaCAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCACCAGCAAAAATGCCg                                                                                                                                     >  7:85510/1‑149 (MQ=255)
                    gcgcCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCgg                                                                                                                                <  6:155204/149‑1 (MQ=255)
                     cgccgcCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTCGCTATCGCCAGCAAAAATGCCGTGCGGc                                                                                                                               <  1:218439/149‑1 (MQ=255)
                        cgccAGCCCCACCAGCATGAACGGCTTCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGa                                                                                                                            <  7:346320/149‑1 (MQ=255)
                        cgccAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGa                                                                                                                            <  2:363090/149‑1 (MQ=255)
                          ccAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATc                                                                                                                                                     <  4:158196/122‑1 (MQ=255)
                          ccAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATc                                                                                                                                                     >  3:158196/1‑122 (MQ=255)
                          ccAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGAt                                                                                                                           >  5:179191/1‑148 (MQ=255)
                          ccAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTACCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGAt                                                                                                                           >  1:195040/1‑148 (MQ=255)
                                 caccaGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGCTGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGCTCACCCCCg                                                                                                                   >  8:76267/1‑149 (MQ=255)
                                 caccaGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCg                                                                                                                   <  3:367921/149‑1 (MQ=255)
                                 caccaGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCg                                                                                                                   >  2:322671/1‑149 (MQ=255)
                                   ccaGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCggg                                                                                                                 <  4:220314/149‑1 (MQ=255)
                                    caGCATGAACTGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCTCTGGCTATCACCAGCAAAAATGCCGTGCGGCTTATCACCCCCGGgc                                                                                                                <  8:85510/149‑1 (MQ=255)
                                      gCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGgcg                                                                                                               >  6:99395/1‑148 (MQ=255)
                                       cATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGgcg                                                                                                               >  1:207413/1‑147 (MQ=255)
                                       cATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGgcg                                                                                                               >  1:213579/1‑147 (MQ=255)
                                           aaCGGCTGCACGTTGTACACCGCCGTTCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGaa                                                                                                          <  3:94071/148‑1 (MQ=255)
                                           aaCGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGaaa                                                                                                         <  4:380768/149‑1 (MQ=255)
                                            aCGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGTCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGaaa                                                                                                         <  6:179191/148‑1 (MQ=255)
                                            aCGGCTGCACGTTGTACACCGCCGTCCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGaaa                                                                                                         <  2:195040/148‑1 (MQ=255)
                                                cTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGCAAGCCaa                                                                                                    >  8:292689/1‑149 (MQ=255)
                                                  gCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAAc                                                                                                  >  8:146418/1‑149 (MQ=255)
                                                   cACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCaaa                                                                                                   <  3:207507/147‑1 (MQ=255)
                                                       ttGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCTTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACgcggc                                                                                             <  2:170129/149‑1 (MQ=255)
                                                       ttGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCa                                                                                                             >  2:330887/1‑133 (MQ=255)
                                                       ttGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCa                                                                                                             <  1:330887/133‑1 (MQ=255)
                                                       ttGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACgcggc                                                                                             <  5:99395/149‑1 (MQ=255)
                                                       ttGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACgcggc                                                                                             >  5:238649/1‑149 (MQ=255)
                                                       ttGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACgcggc                                                                                             >  5:233692/1‑149 (MQ=255)
                                                         gTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACgcggcg                                                                                            >  8:258011/1‑148 (MQ=255)
                                                             acCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGCTCACCCCCGGCCGCAGAAAGCCACACGCGGCGCaact                                                                                       >  5:238397/1‑147 (MQ=255)
                                                                  cgTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCa                                                                                  <  2:234568/149‑1 (MQ=255)
                                                                      ccGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGc                                                                                >  3:130262/1‑147 (MQ=255)
                                                                       cGATGGCAATCGCCGCCCGGGCGTTTGAAGCTAACAACAGCAGCCAGTTGCCGACAATGGCCACCGCGCGGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAg                                                                             <  6:238397/149‑1 (MQ=255)
                                                                       cGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAg                                                                             >  4:138264/1‑149 (MQ=255)
                                                                        gATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAACGCCAAACGCGGCGCAAATCGCCAGCAAgg                                                                            >  6:220899/1‑149 (MQ=255)
                                                                             cAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAAcgcg                                                                                               >  2:203641/1‑125 (MQ=255)
                                                                             cAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAAcgcg                                                                                               <  1:203641/125‑1 (MQ=255)
                                                                              aaTCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACgg                                                                      >  2:118381/1‑149 (MQ=255)
                                                                                 cGACGCCCGGGAGTATGAAGCGAACAACAGCAGACAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACAACCGGGCGCACAAAGCCAAACGCGGCGCAAATAGCCAGCAAGGTCACGGCAc                                                                   >  5:223958/1‑149 (MQ=255)
                                                                                   aCGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCg                                                                 <  6:238649/149‑1 (MQ=255)
                                                                                     gCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGaa                                                               >  6:329829/1‑149 (MQ=255)
                                                                                     gCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCCGCGCACATCGCCACCAAGGTCACGGCACCGa                                                                >  2:380011/1‑148 (MQ=255)
                                                                                        cGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATAc                                                            >  3:127330/1‑149 (MQ=255)
                                                                                             gTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAgc                                                       >  8:106322/1‑149 (MQ=255)
                                                                                             gTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAgc                                                       <  1:118381/149‑1 (MQ=255)
                                                                                             gTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAgc                                                       <  3:88854/149‑1 (MQ=255)
                                                                                                tGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAgcgc                                                     <  2:213579/148‑1 (MQ=255)
                                                                                                tGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAgcgc                                                     <  2:207413/148‑1 (MQ=255)
                                                                                                    gCGAACAACAGCAGCCAGTTGCCGACAGTGGCCACCCCGCTGGGTATGGGCAGCAAAAATGCCGTGCGGGTGCTCAGCCCCGGGCGCGGTAAGCGATGCGCGGCGCATGTCGCCAGCAAGGTGCCGGCGCCGATTCCGCAgcggccgag                                                >  8:66505/1‑143 (MQ=255)
                                                                                                      gAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAac                                               <  2:77468/148‑1 (MQ=255)
                                                                                                      gAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAac                                               >  1:77468/1‑148 (MQ=255)
                                                                                                       aacaacAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAacac                                             >  3:136738/1‑149 (MQ=255)
                                                                                                       aacaacAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAacac                                             >  7:300678/1‑149 (MQ=255)
                                                                                                        acaacaGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAAcacc                                            <  1:380011/149‑1 (MQ=255)
                                                                                                        acaacaGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAAcacc                                            <  6:233692/149‑1 (MQ=255)
                                                                                                         caacaGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAAcacca                                           <  8:213465/149‑1 (MQ=255)
                                                                                                         caacaGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAAcacca                                           <  7:146418/149‑1 (MQ=255)
                                                                                                                agcCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAg                                    <  7:76267/149‑1 (MQ=255)
                                                                                                                  ccAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGTGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCa                                  >  6:263539/1‑149 (MQ=255)
                                                                                                                      ttGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCgg                              <  5:329829/149‑1 (MQ=255)
                                                                                                                          cGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTgg                          >  6:297578/1‑149 (MQ=255)
                                                                                                                          cGACAATGGCCACCCCGCTGGCTATCGCCAGAAAAAATGCCGAGCGGCTGATCACCCCCGGGCGCAGAAAGCCACACGCGGCGCAAATCTCCCGCAAGGTCACGGCACCGAATACGCAGCGCCAGCACACCACAACCAGAACCGCCTgg                          >  3:251363/1‑149 (MQ=255)
                                                                                                                            aCAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGcc                        <  7:106322/149‑1 (MQ=255)
                                                                                                                            aCAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGcc                        >  6:9289/1‑149 (MQ=255)
                                                                                                                             cAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCg                       <  4:127330/149‑1 (MQ=255)
                                                                                                                               aTGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCGGa                     <  5:9289/149‑1 (MQ=255)
                                                                                                                                 ggCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCACCGCCAGAACACCACATCCAGCACCGGCTCGCCGGaca                   >  5:69403/1‑149 (MQ=255)
                                                                                                                                   ccACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATcc                                      <  3:354051/128‑1 (MQ=255)
                                                                                                                                   ccACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATcc                                      >  4:354051/1‑128 (MQ=255)
                                                                                                                                   ccACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCGGACAcc                 <  4:113963/149‑1 (MQ=255)
                                                                                                                                    cACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCGGACACCa                <  1:360285/149‑1 (MQ=255)
                                                                                                                                     tttttgCTGGCGATAGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCg                       >  4:320182/6‑141 (MQ=255)
                                                                                                                                     tttttgCTGGCGATAGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCg                       <  3:320182/136‑1 (MQ=255)
                                                                                                                                       cccGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCGGACACCAGCa             >  6:229385/1‑149 (MQ=255)
                                                                                                                                         cGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAAcacca                                           >  7:326635/1‑117 (MQ=255)
                                                                                                                                         cGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAAcacca                                           <  8:326635/117‑1 (MQ=255)
                                                                                                                                           cTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCTCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACTGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCGGACACCAGCACGaa         <  5:23867/149‑1 (MQ=255)
                                                                                                                                                  tCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCGGACACCAGCACGAACCAGcc   >  6:6344/1‑148 (MQ=255)
                                                                                                                                                  tCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCGGACACCAGCACGAACCAGcc   <  1:307256/148‑1 (MQ=255)
                                                                                                                                                  tCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCGGACACCAGCACGAACCAGCCa  <  4:136738/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATCTTTTCGCCCAGAAACAGCGCCGCCAGCCCCACCAGCATGAACGGCTGCACGTTGTACACCGCCGTGCCGATGGCAATCGACGCCCGGGCGTATGAAGCGAACAACAGCAGCCAGTTGCCGACAATGGCCACCCCGCTGGCTATCGCCAGCAAAAATGCCGTGCGGCTGATCACCCCCGGGCGCAGAAAGCCAAACGCGGCGCAAATCGCCAGCAAGGTCACGGCACCGAATACGCAGCGCCAGAACACCACATCCAGCACCGGCTGGCCGGACACCAGCACGAACCAGCCA  >  NC_002947/4899109‑4899403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑