Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,089,074 G→C 5.3% G255G (GGC→GGG)  astA‑I ← arginine N‑succinyltransferase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,089,0740GC 5.3% 242.4 / 3.8 76G255G (GGC→GGG) astA‑Iarginine N‑succinyltransferase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (35/36);  new base C (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

CAGGTGCGGCAGTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTGG  >  NC_002947/5088926‑5089219
                                                                                                                                                    |                                                                                                                                                  
cAGGTGAGGCTGTGATGCGTCTGTGGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCAGACTCGATggccggg                                                                                                                                                    <  6:16608/149‑1 (MQ=255)
   gTGCGGCAGTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATggccgggccg                                                                                                                                                 <  1:3034/149‑1 (MQ=255)
          aGTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCg                                                                                                                                          >  7:6823/1‑148 (MQ=255)
           gTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGaa                                                                                                                                        <  6:166161/149‑1 (MQ=255)
           gTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGCCCGGCCATCGa                                                                                                                                         <  3:347807/149‑1 (MQ=255)
           gTGATGCGACACTCCTCGCGATTGCGGTTGTGGAGGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGAT‑GCCGGCCCGGCCATCGag                                                                                                                                        <  4:224657/149‑2 (MQ=255)
              aTGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGa                                                                                                                                      <  4:322377/148‑1 (MQ=255)
              aTGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGa                                                                                                                                      <  6:21714/148‑1 (MQ=255)
              aTGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGAt                                                                                                                                     <  3:294484/149‑1 (MQ=255)
                  gACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTc                                                                                                                                  >  1:68242/1‑148 (MQ=255)
                         gtcgCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGc                                                                                                                          >  6:391196/1‑149 (MQ=255)
                          tcgCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGcc                                                                                                                         >  6:90670/1‑149 (MQ=255)
                          tcgCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGcc                                                                                                                         >  6:302106/1‑149 (MQ=255)
                            gcgcTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGccc                                                                                                                        <  2:68242/148‑1 (MQ=255)
                            gcgcTTGCGGGTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCt                                                                                                                       <  8:240054/149‑1 (MQ=255)
                              gcTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTgg                                                                                                                     >  7:78344/1‑149 (MQ=255)
                                    ggTTGTGGATTATGAAAGGGGTCGCGTCATCGCCTGTCGTAGCTACCGCCAGCACCAGGGTCTGGCTGTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGtt                                                                                                                <  8:6823/148‑1 (MQ=255)
                                    ggTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTg                                                                                                               <  1:13997/149‑1 (MQ=255)
                                      ttGTGGATGATGAAAGAGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGaa                                                                                                             <  7:284447/149‑1 (MQ=255)
                                           gatgatGACAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCtt                                                                                                        <  8:75550/149‑1 (MQ=255)
                                            atgatgAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTc                                                                                                       <  2:28651/149‑1 (MQ=255)
                                                 gaAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAAGGTCTGGCTCTAGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCt                                                                                                  >  1:79395/1‑149 (MQ=255)
                                                  aaaGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCtt                                                                                                 <  5:391196/149‑1 (MQ=255)
                                                  aaaGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCt                                                                                                  <  1:195252/148‑1 (MQ=255)
                                                    aGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCtt                                                                                                 >  2:367187/1‑147 (MQ=255)
                                                            cgTCNTCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGc                                                                                        >  5:26934/1‑148 (MQ=255)
                                                             gTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGt                                                                                                                                   <  4:34040/104‑1 (MQ=255)
                                                             gTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGt                                                                                                                                   >  3:34040/1‑104 (MQ=255)
                                                              tCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAg                                                                                     >  5:289279/1‑149 (MQ=255)
                                                               cATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAgc                                                                                    >  8:87520/1‑149 (MQ=255)
                                                                aTCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAgcg                                                                                   >  6:247295/1‑149 (MQ=255)
                                                                 tCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAgcgc                                                                                  >  8:67916/1‑149 (MQ=255)
                                                                   gCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAg                                                                                                                  >  7:121272/1‑115 (MQ=255)
                                                                   gCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAg                                                                                                                  <  8:121272/115‑1 (MQ=255)
                                                                   gCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGcc                                                                                 <  8:78344/148‑1 (MQ=255)
                                                                   gCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCg                                                                                >  8:228206/1‑149 (MQ=255)
                                                                      ttgCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCt                                                                             >  7:249096/3‑149 (MQ=255)
                                                                        gCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCg                                                                           <  2:66256/149‑1 (MQ=255)
                                                                          gTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGgt                                                                         <  2:336677/149‑1 (MQ=255)
                                                                          gTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGgt                                                                         <  8:222845/149‑1 (MQ=255)
                                                                            acctaccGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGgtgt                                                                       >  2:223118/1‑149 (MQ=255)
                                                                              ctaccGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTgg                                                                                                                     <  3:197420/101‑1 (MQ=255)
                                                                              ctaccGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCt                                                                     <  7:67916/149‑1 (MQ=255)
                                                                              ctaccGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCt                                                                     >  6:13996/1‑149 (MQ=255)
                                                                              ctaccGCCAGCACCAGGGTCTGGCTCGCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTgg                                                                                                                     >  4:197420/1‑101 (MQ=255)
                                                                                accGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCttt                                                                   >  2:247234/1‑149 (MQ=255)
                                                                                  cGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgt                                                                 <  2:353888/149‑1 (MQ=255)
                                                                                    ccAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgtg                                                                >  1:214059/1‑148 (MQ=255)
                                                                                     cAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGca                                                              <  2:214059/149‑1 (MQ=255)
                                                                                          ccAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgt                                                                 <  1:255017/141‑1 (MQ=255)
                                                                                          ccAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgt                                                                 >  2:255017/1‑141 (MQ=255)
                                                                                           cAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGc                                                        >  2:150176/1‑149 (MQ=255)
                                                                                           cAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACCTAGCCCTGGTAGTTGAAGCCTTACTGCTTGAGCATGGCCAGCGCCGGCTAGGTGTCTTTGTGCACACGGc                                                        >  3:216433/1‑149 (MQ=255)
                                                                                               gTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGAt                                                    <  5:13996/149‑1 (MQ=255)
                                                                                                tCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATc                                                   <  6:26934/149‑1 (MQ=255)
                                                                                                    gCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgt                                                                 >  4:313443/1‑131 (MQ=255)
                                                                                                    gCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgt                                                                 <  3:313443/131‑1 (MQ=255)
                                                                                                    gCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCacac                                               >  1:175773/1‑149 (MQ=255)
                                                                                                     ctctCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCg                                                                           >  5:162537/1‑120 (MQ=255)
                                                                                                     ctctCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCg                                                                           <  6:162537/120‑1 (MQ=255)
                                                                                                     ctctCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACt                                              <  1:150176/149‑1 (MQ=255)
                                                                                                      tctcGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGa                                                                                               >  3:344232/1‑99 (MQ=255)
                                                                                                      tctcGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGa                                                                                               <  4:344232/99‑1 (MQ=255)
                                                                                                          gcgTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTgcgcg                                         >  3:175723/1‑149 (MQ=255)
                                                                                                                ggCGCGGATCTTGGCAGTGTCACACTCGATGGCCGAGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCtt                                   >  1:57660/1‑149 (MQ=255)
                                                                                                                   gcgGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCg                                 >  3:176794/1‑148 (MQ=255)
                                                                                                                    cgGATCTTGGCAGTGTCACACTCCATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGa                               <  6:171135/149‑1 (MQ=255)
                                                                                                                      gATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACa                             <  2:79395/149‑1 (MQ=255)
                                                                                                                      gATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACa                             <  1:223118/149‑1 (MQ=255)
                                                                                                                         cTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAgg                           >  7:148928/1‑148 (MQ=255)
                                                                                                                               aGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAgg                    >  1:266410/1‑149 (MQ=255)
                                                                                                                                 tgtCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGgtg                  <  4:60544/149‑1 (MQ=255)
                                                                                                                                   tCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGgtgt                 <  5:247295/148‑1 (MQ=255)
                                                                                                                                   tCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTa                <  1:367187/149‑1 (MQ=255)
                                                                                                                                     atcttCGAT‑GCCGGCCCGGCCATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTa                >  5:185310/5‑147 (MQ=255)
                                                                                                                                     acacTCGATGGCCGGTCCGG‑CATCGAAGATGTCGACGTAGACCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACa              >  7:107061/1‑149 (MQ=255)
                                                                                                                                     acacTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTa                >  3:158354/1‑147 (MQ=255)
                                                                                                                                      tcttCGAT‑GCCGGCCCGGCCATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTTTACAg             >  3:56265/4‑149 (MQ=255)
                                                                                                                                                ccgggccgg‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTg   <  2:266410/149‑1 (MQ=255)
                                                                                                                                                ccgggccgg‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTg   >  6:223665/1‑149 (MQ=255)
                                                                                                                                                 cgggccgg‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTgg  <  7:87520/149‑1 (MQ=255)
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CAGGTGCGGCAGTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCTGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTGG  >  NC_002947/5088926‑5089219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑