Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,108,454 T→C 6.4% L64P (CTG→CCG) ‡ PP_4494 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,108,4540TC 6.4% 185.7 / 2.8 64L64P (CTG→CCG) ‡PP_4494LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (31/28);  new base C (2/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

CTTTCGAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTT  >  NC_002947/5108307‑5108601
                                                                                                                                                   |                                                                                                                                                   
cTTTCGAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCt                                                                                                                                                     >  1:188406/1‑148 (MQ=255)
  ttCGAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGAc                                                                                                                                                  >  1:222151/1‑149 (MQ=255)
    cGAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGAcc                                                                                                                                                 >  5:104915/1‑148 (MQ=255)
     gAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGa                                                                                                                                               >  1:55985/1‑149 (MQ=255)
             cgccCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGgcc                                                                                                                                       <  2:358909/149‑1 (MQ=255)
                ccGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGgccgcc                                                                                                                                    >  2:158861/1‑149 (MQ=255)
                        acaGCCACACCCGCTCCGCCGCCGAACTGTCTCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGGTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCa                                                                                                                             <  4:302855/146‑1 (MQ=255)
                        aaaGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGGCGGCCGACATCGGTCAGCTGTAcctg                                                                                                                            <  7:217014/149‑3 (MQ=255)
                        aaaGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCa                                                                                                                             <  3:113177/148‑1 (MQ=255)
                           gCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCACGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAgcgc                                                                                                                         <  8:307013/149‑1 (MQ=255)
                             tACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGa                                                                                                                       >  6:212855/1‑149 (MQ=255)
                               cacCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACTGATGTCGGCCGCCTGTACCAGCGCGa                                                                                                                       <  1:5799/147‑1 (MQ=255)
                                acCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCt                                                                                                                    >  7:146086/1‑149 (MQ=255)
                                 cccGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCt                                                                                                                    >  1:318182/1‑148 (MQ=255)
                                  ccGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGa                                                                                                                  <  4:91439/149‑1 (MQ=255)
                                    gcgcCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                <  2:222151/149‑1 (MQ=255)
                                    gcgcCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGAAGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                >  4:34902/1‑149 (MQ=255)
                                     cgccgccgcCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCg                                                                                                               <  2:188406/149‑1 (MQ=255)
                                        cgacgccgAACTTTCGCTGACCCAAAGCGCGGTCATCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTTAACCTGTTCCTCAGCTAGGGGAGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGa                                                                                                              <  6:168109/144‑1 (MQ=255)
                                          ccgccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGGCGGCCGACATCGGTCAGCTGTAcct                                                                                                                             <  6:64643/130‑2 (MQ=38)
                                          ccgccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGGCGGCCGACATCGGGCAGCTGTAcct                                                                                                                             >  5:64643/1‑129 (MQ=255)
                                          ccgccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCa                                                                                                          <  7:179381/149‑1 (MQ=255)
                                            gccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGa                                                                                                                  <  3:148091/139‑1 (MQ=255)
                                            gccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGa                                                                                                                  >  4:148091/1‑139 (MQ=255)
                                              cgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTgg                                                                                                      <  3:49558/149‑1 (MQ=255)
                                              cgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTgg                                                                                                      >  7:227364/1‑149 (MQ=255)
                                                aaCTGTCGCTGACCCAAAGCGCGGTCAGCTGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTgggg                                                                                                    <  7:224263/149‑1 (MQ=255)
                                                   tGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGgcg                                                                                                  >  5:286730/1‑148 (MQ=255)
                                                            cccAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAgc                                                                                        <  6:212770/149‑1 (MQ=255)
                                                            cccAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAgc                                                                                        >  5:212770/1‑149 (MQ=255)
                                                             ccAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAgcg                                                                                       <  3:34902/149‑1 (MQ=255)
                                                              cAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                <  4:285016/123‑1 (MQ=255)
                                                              cAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                >  3:285016/1‑123 (MQ=255)
                                                                  gcgcGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCAcc                                                                                  >  6:188177/1‑149 (MQ=255)
                                                                      ggTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGc                                                                              <  6:104915/149‑1 (MQ=255)
                                                                        tCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCAGCCTGTACCAGCGCGAGCTGAGCGAGGCACTCGGGCGCATCCGCAGCGCCACCTTGCAg                                                                            >  8:296704/1‑149 (MQ=255)
                                                                            ccgccAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCa                                                                         >  1:138553/1‑148 (MQ=255)
                                                                            ccgccAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCAc                                                                        >  8:194374/1‑149 (MQ=255)
                                                                                cAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGc                                                                    <  2:55985/149‑1 (MQ=255)
                                                                                   gTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTa                                                                 <  5:212855/149‑1 (MQ=255)
                                                                                         gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTa                                                                 >  5:249127/1‑143 (MQ=255)
                                                                                         gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTa                                                                 <  6:249127/143‑1 (MQ=255)
                                                                                         gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATc                                                           >  8:239090/1‑149 (MQ=255)
                                                                                         gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATc                                                           <  6:286730/149‑1 (MQ=255)
                                                                                           ccTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCgg                                                         <  7:332340/149‑1 (MQ=255)
                                                                                           ccTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCgg                                                         <  8:329837/149‑1 (MQ=255)
                                                                                           ccTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCgg                                                         <  5:188177/149‑1 (MQ=255)
                                                                                               gAGCAGCAGTTGGTCCGGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGa                                                       <  4:230793/147‑1 (MQ=255)
                                                                                               gAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAg                                                      >  5:172436/1‑148 (MQ=255)
                                                                                                                 aCCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGc                                   >  2:131144/1‑149 (MQ=255)
                                                                                                                 aCCCTGTTCAGCCGCGAGGGGCGCAAGGTACAGCAGACAGATGTCGGCCGCCTATACAAGCGCGAGCAGAGCGAGGAACTGGGGCGCCTCCGCAGCGCCACCTTGCAGGCACTCGCCAATCAATCGGGAGTAGGCACCATGCGCCTgg                                    >  5:98580/1‑148 (MQ=255)
                                                                                                                   ccTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCa                                 >  2:26748/1‑149 (MQ=255)
                                                                                                                      gTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCAccc                              >  1:204247/1‑149 (MQ=255)
                                                                                                                       ttCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCCCCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCt                             >  5:209573/1‑149 (MQ=255)
                                                                                                                                         ctggTACAGGCGGCCGACATCGGTCAGCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACCTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGa            >  7:266964/3‑148 (MQ=255)
                                                                                                                                         cAGGTACAGCTGCCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGa            >  5:265218/1‑148 (MQ=255)
                                                                                                                                         cAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGa            >  2:243865/1‑148 (MQ=255)
                                                                                                                                            gTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATg        >  3:233983/1‑149 (MQ=255)
                                                                                                                                             tACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATgg       <  8:227364/149‑1 (MQ=255)
                                                                                                                                              aCAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGc      >  2:324805/1‑149 (MQ=255)
                                                                                                                                               cAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCt     <  7:194374/149‑1 (MQ=255)
                                                                                                                                               cAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCt     <  7:239090/149‑1 (MQ=255)
                                                                                                                                                aGCTGAACGATGTCGGCCGCCTGTACCAGCGCCAGCAGAGAGAGGCACTGGGGCGCATCCGCCGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGCCACTTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATggatc    >  4:360360/1‑146 (MQ=255)
                                                                                                                                                 gCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGt   >  6:246876/1‑149 (MQ=255)
                                                                                                                                                  cTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGtt  >  5:23594/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTTTCGAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTT  >  NC_002947/5108307‑5108601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑