Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,108,456 A→G 6.2% T65A (ACC→GCC)  PP_4494 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,108,4560AG 6.2% 187.3 / 2.5 65T65A (ACC→GCC) PP_4494LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (32/28);  new base G (2/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

TTCGAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGCC  >  NC_002947/5108309‑5108604
                                                                                                                                                   |                                                                                                                                                    
ttCGAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGAc                                                                                                                                                     >  1:222151/1‑149 (MQ=255)
  cGAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGAcc                                                                                                                                                    >  5:104915/1‑148 (MQ=255)
   gAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGa                                                                                                                                                  >  1:55985/1‑149 (MQ=255)
           cgccCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGgcc                                                                                                                                          <  2:358909/149‑1 (MQ=255)
              ccGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGgccgcc                                                                                                                                       >  2:158861/1‑149 (MQ=255)
                      acaGCCACACCCGCTCCGCCGCCGAACTGTCTCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGGTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCa                                                                                                                                <  4:302855/146‑1 (MQ=255)
                      aaaGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGGCGGCCGACATCGGTCAGCTGTAcctg                                                                                                                               <  7:217014/149‑3 (MQ=255)
                      aaaGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCa                                                                                                                                <  3:113177/148‑1 (MQ=255)
                         gCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCACGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAgcgc                                                                                                                            <  8:307013/149‑1 (MQ=255)
                           tACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGa                                                                                                                          >  6:212855/1‑149 (MQ=255)
                             cacCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACTGATGTCGGCCGCCTGTACCAGCGCGa                                                                                                                          <  1:5799/147‑1 (MQ=255)
                              acCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCt                                                                                                                       >  7:146086/1‑149 (MQ=255)
                               cccGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCt                                                                                                                       >  1:318182/1‑148 (MQ=255)
                                ccGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGa                                                                                                                     <  4:91439/149‑1 (MQ=255)
                                  gcgcCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                   <  2:222151/149‑1 (MQ=255)
                                  gcgcCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGAAGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                   >  4:34902/1‑149 (MQ=255)
                                   cgccgccgcCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCg                                                                                                                  <  2:188406/149‑1 (MQ=255)
                                      cgacgccgAACTTTCGCTGACCCAAAGCGCGGTCATCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTTAACCTGTTCCTCAGCTAGGGGAGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGa                                                                                                                 <  6:168109/144‑1 (MQ=255)
                                        ccgccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGGCGGCCGACATCGGTCAGCTGTAcct                                                                                                                                <  6:64643/130‑2 (MQ=38)
                                        ccgccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGGCGGCCGACATCGGGCAGCTGTAcct                                                                                                                                >  5:64643/1‑129 (MQ=255)
                                        ccgccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCa                                                                                                             <  7:179381/149‑1 (MQ=255)
                                          gccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGa                                                                                                                     <  3:148091/139‑1 (MQ=255)
                                          gccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGa                                                                                                                     >  4:148091/1‑139 (MQ=255)
                                            cgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTgg                                                                                                         >  7:227364/1‑149 (MQ=255)
                                            cgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTgg                                                                                                         <  3:49558/149‑1 (MQ=255)
                                              aaCTGTCGCTGACCCAAAGCGCGGTCAGCTGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTgggg                                                                                                       <  7:224263/149‑1 (MQ=255)
                                                 tGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGgcg                                                                                                     >  5:286730/1‑148 (MQ=255)
                                                          cccAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAgc                                                                                           <  6:212770/149‑1 (MQ=255)
                                                          cccAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAgc                                                                                           >  5:212770/1‑149 (MQ=255)
                                                           ccAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAgcg                                                                                          <  3:34902/149‑1 (MQ=255)
                                                            cAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                   >  3:285016/1‑123 (MQ=255)
                                                            cAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                   <  4:285016/123‑1 (MQ=255)
                                                                gcgcGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCAcc                                                                                     >  6:188177/1‑149 (MQ=255)
                                                                    ggTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGc                                                                                 <  6:104915/149‑1 (MQ=255)
                                                                      tCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCAGCCTGTACCAGCGCGAGCTGAGCGAGGCACTCGGGCGCATCCGCAGCGCCACCTTGCAg                                                                               >  8:296704/1‑149 (MQ=255)
                                                                          ccgccAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCa                                                                            >  1:138553/1‑148 (MQ=255)
                                                                          ccgccAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCAc                                                                           >  8:194374/1‑149 (MQ=255)
                                                                              cAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGc                                                                       <  2:55985/149‑1 (MQ=255)
                                                                                 gTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTa                                                                    <  5:212855/149‑1 (MQ=255)
                                                                                       gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTa                                                                    <  6:249127/143‑1 (MQ=255)
                                                                                       gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTa                                                                    >  5:249127/1‑143 (MQ=255)
                                                                                       gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATc                                                              >  8:239090/1‑149 (MQ=255)
                                                                                       gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATc                                                              <  6:286730/149‑1 (MQ=255)
                                                                                         ccTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCgg                                                            <  7:332340/149‑1 (MQ=255)
                                                                                         ccTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCgg                                                            <  8:329837/149‑1 (MQ=255)
                                                                                         ccTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCgg                                                            <  5:188177/149‑1 (MQ=255)
                                                                                             gAGCAGCAGTTGGTCCGGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGa                                                          <  4:230793/147‑1 (MQ=255)
                                                                                             gAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAg                                                         >  5:172436/1‑148 (MQ=255)
                                                                                                               aCCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGc                                      >  2:131144/1‑149 (MQ=255)
                                                                                                               aCCCTGTTCAGCCGCGAGGGGCGCAAGGTACAGCAGACAGATGTCGGCCGCCTATACAAGCGCGAGCAGAGCGAGGAACTGGGGCGCCTCCGCAGCGCCACCTTGCAGGCACTCGCCAATCAATCGGGAGTAGGCACCATGCGCCTgg                                       >  5:98580/1‑148 (MQ=255)
                                                                                                                 ccTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCa                                    >  2:26748/1‑149 (MQ=255)
                                                                                                                    gTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCAccc                                 >  1:204247/1‑149 (MQ=255)
                                                                                                                     ttCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCCCCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCt                                >  5:209573/1‑149 (MQ=255)
                                                                                                                                       ctggTACAGGCGGCCGACATCGGTCAGCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACCTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGa               >  7:266964/3‑148 (MQ=255)
                                                                                                                                       cAGGTACAGCTGCCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGa               >  5:265218/1‑148 (MQ=255)
                                                                                                                                       cAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGa               >  2:243865/1‑148 (MQ=255)
                                                                                                                                          gTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATg           >  3:233983/1‑149 (MQ=255)
                                                                                                                                           tACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATgg          <  8:227364/149‑1 (MQ=255)
                                                                                                                                            aCAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGc         >  2:324805/1‑149 (MQ=255)
                                                                                                                                             cAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCt        <  7:194374/149‑1 (MQ=255)
                                                                                                                                             cAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCt        <  7:239090/149‑1 (MQ=255)
                                                                                                                                              aGCTGAACGATGTCGGCCGCCTGTACCAGCGCCAGCAGAGAGAGGCACTGGGGCGCATCCGCCGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGCCACTTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATggatc       >  4:360360/1‑146 (MQ=255)
                                                                                                                                               gCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGt      >  6:246876/1‑149 (MQ=255)
                                                                                                                                                cTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGtt     >  5:23594/1‑149 (MQ=255)
                                                                                                                                                  gACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGc   >  3:294274/1‑149 (MQ=255)
                                                                                                                                                   aCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCAcc                                  <  2:146521/117‑1 (MQ=255)
                                                                                                                                                   aCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCAcc                                  >  1:146521/1‑117 (MQ=255)
                                                                                                                                                   aCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGt      <  2:118880/145‑1 (MQ=255)
                                                                                                                                                   aCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGt      >  1:118880/1‑145 (MQ=255)
                                                                                                                                                   aCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGcc  >  1:226685/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTCGAGGCCGCCGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGCC  >  NC_002947/5108309‑5108604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑