Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,108,461 T→C 5.5% D66D (GAT→GAC)  PP_4494 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,108,4610TC 5.5% 218.3 / 2.5 74D66D (GAT→GAC) PP_4494LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base C (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

CGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGCCGAGG  >  NC_002947/5108320‑5108608
                                                                                                                                             |                                                                                                                                                   
cgccCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGgcc                                                                                                                                              <  2:358909/149‑1 (MQ=255)
   ccGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGgccgcc                                                                                                                                           >  2:158861/1‑149 (MQ=255)
           acaGCCACACCCGCTCCGCCGCCGAACTGTCTCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGGTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCa                                                                                                                                    <  4:302855/146‑1 (MQ=255)
           aaaGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGGCGGCCGACATCGGTCAGCTGTAcctg                                                                                                                                   <  7:217014/149‑3 (MQ=255)
           aaaGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCa                                                                                                                                    <  3:113177/148‑1 (MQ=255)
              gCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCACGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAgcgc                                                                                                                                <  8:307013/149‑1 (MQ=255)
                tACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGa                                                                                                                              >  6:212855/1‑149 (MQ=255)
                  cacCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACTGATGTCGGCCGCCTGTACCAGCGCGa                                                                                                                              <  1:5799/147‑1 (MQ=255)
                   acCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCt                                                                                                                           >  7:146086/1‑149 (MQ=255)
                    cccGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCt                                                                                                                           >  1:318182/1‑148 (MQ=255)
                     ccGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGa                                                                                                                         <  4:91439/149‑1 (MQ=255)
                       gcgcCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                       <  2:222151/149‑1 (MQ=255)
                       gcgcCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGAAGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                       >  4:34902/1‑149 (MQ=255)
                        cgccgccgcCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCg                                                                                                                      <  2:188406/149‑1 (MQ=255)
                           cgacgccgAACTTTCGCTGACCCAAAGCGCGGTCATCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTTAACCTGTTCCTCAGCTAGGGGAGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGa                                                                                                                     <  6:168109/144‑1 (MQ=255)
                             ccgccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGGCGGCCGACATCGGTCAGCTGTAcct                                                                                                                                    <  6:64643/130‑2 (MQ=38)
                             ccgccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGGCGGCCGACATCGGGCAGCTGTAcct                                                                                                                                    >  5:64643/1‑129 (MQ=255)
                             ccgccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCa                                                                                                                 <  7:179381/149‑1 (MQ=255)
                               gccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGa                                                                                                                         <  3:148091/139‑1 (MQ=255)
                               gccgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGa                                                                                                                         >  4:148091/1‑139 (MQ=255)
                                 cgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTgg                                                                                                             >  7:227364/1‑149 (MQ=255)
                                 cgAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTgg                                                                                                             <  3:49558/149‑1 (MQ=255)
                                   aaCTGTCGCTGACCCAAAGCGCGGTCAGCTGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTgggg                                                                                                           <  7:224263/149‑1 (MQ=255)
                                      tGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGgcg                                                                                                         >  5:286730/1‑148 (MQ=255)
                                               cccAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAgc                                                                                               >  5:212770/1‑149 (MQ=255)
                                               cccAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAgc                                                                                               <  6:212770/149‑1 (MQ=255)
                                                ccAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAgcg                                                                                              <  3:34902/149‑1 (MQ=255)
                                                 cAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                       >  3:285016/1‑123 (MQ=255)
                                                 cAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGc                                                                                                                       <  4:285016/123‑1 (MQ=255)
                                                     gcgcGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCAcc                                                                                         >  6:188177/1‑149 (MQ=255)
                                                         ggTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGc                                                                                     <  6:104915/149‑1 (MQ=255)
                                                           tCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCAGCCTGTACCAGCGCGAGCTGAGCGAGGCACTCGGGCGCATCCGCAGCGCCACCTTGCAg                                                                                   >  8:296704/1‑149 (MQ=255)
                                                               ccgccAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCa                                                                                >  1:138553/1‑148 (MQ=255)
                                                               ccgccAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCAc                                                                               >  8:194374/1‑149 (MQ=255)
                                                                   cAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGc                                                                           <  2:55985/149‑1 (MQ=255)
                                                                      gTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTa                                                                        <  5:212855/149‑1 (MQ=255)
                                                                            gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTa                                                                        <  6:249127/143‑1 (MQ=255)
                                                                            gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTa                                                                        >  5:249127/1‑143 (MQ=255)
                                                                            gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATc                                                                  <  6:286730/149‑1 (MQ=255)
                                                                            gCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATc                                                                  >  8:239090/1‑149 (MQ=255)
                                                                              ccTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCgg                                                                <  7:332340/149‑1 (MQ=255)
                                                                              ccTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCgg                                                                <  8:329837/149‑1 (MQ=255)
                                                                              ccTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCgg                                                                <  5:188177/149‑1 (MQ=255)
                                                                                  gAGCAGCAGTTGGTCCGGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGa                                                              <  4:230793/147‑1 (MQ=255)
                                                                                  gAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAg                                                             >  5:172436/1‑148 (MQ=255)
                                                                                                    aCCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGc                                          >  2:131144/1‑149 (MQ=255)
                                                                                                    aCCCTGTTCAGCCGCGAGGGGCGCAAGGTACAGCAGACAGATGTCGGCCGCCTATACAAGCGCGAGCAGAGCGAGGAACTGGGGCGCCTCCGCAGCGCCACCTTGCAGGCACTCGCCAATCAATCGGGAGTAGGCACCATGCGCCTgg                                           >  5:98580/1‑148 (MQ=255)
                                                                                                      ccTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCa                                        >  2:26748/1‑149 (MQ=255)
                                                                                                         gTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCAccc                                     >  1:204247/1‑149 (MQ=255)
                                                                                                          ttCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCCCCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCt                                    >  5:209573/1‑149 (MQ=255)
                                                                                                                            ctggTACAGGCGGCCGACATCGGTCAGCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACCTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGa                   >  7:266964/3‑148 (MQ=255)
                                                                                                                            cAGGTACAGCTGCCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGa                   >  5:265218/1‑148 (MQ=255)
                                                                                                                            cAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGa                   >  2:243865/1‑148 (MQ=255)
                                                                                                                               gTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATg               >  3:233983/1‑149 (MQ=255)
                                                                                                                                tACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATgg              <  8:227364/149‑1 (MQ=255)
                                                                                                                                 aCAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGc             >  2:324805/1‑149 (MQ=255)
                                                                                                                                  cAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCt            <  7:239090/149‑1 (MQ=255)
                                                                                                                                  cAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCt            <  7:194374/149‑1 (MQ=255)
                                                                                                                                   aGCTGAACGATGTCGGCCGCCTGTACCAGCGCCAGCAGAGAGAGGCACTGGGGCGCATCCGCCGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGCCACTTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATggatc           >  4:360360/1‑146 (MQ=255)
                                                                                                                                    gCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGt          >  6:246876/1‑149 (MQ=255)
                                                                                                                                     cTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGtt         >  5:23594/1‑149 (MQ=255)
                                                                                                                                       gACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGc       >  3:294274/1‑149 (MQ=255)
                                                                                                                                        aCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCAcc                                      <  2:146521/117‑1 (MQ=255)
                                                                                                                                        aCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCAcc                                      >  1:146521/1‑117 (MQ=255)
                                                                                                                                        aCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGt          >  1:118880/1‑145 (MQ=255)
                                                                                                                                        aCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGt          <  2:118880/145‑1 (MQ=255)
                                                                                                                                        aCCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGcc      >  1:226685/1‑149 (MQ=255)
                                                                                                                                         ccGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGCCg     <  5:246876/149‑1 (MQ=255)
                                                                                                                                          cGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGcc      >  3:222293/1‑147 (MQ=255)
                                                                                                                                          cGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGCCGa    <  1:158861/149‑1 (MQ=255)
                                                                                                                                          cGATGGCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGCCGa    <  2:138553/149‑1 (MQ=255)
                                                                                                                                            aTGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTa                                                           >  7:92223/1‑92 (MQ=255)
                                                                                                                                            aTGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTa                                                           <  8:92223/92‑1 (MQ=255)
                                                                                                                                            aTGGCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGCCGAgg  <  2:318182/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CGCCCGGCATGAAAGCTACACCCGCGCCGCCGCCGAACTGTCGCTGACCCAAAGCGCGGTCAGCCGCCAGGTACAGGCCCTGGAGCAGCAGTTGGGCCTGACCCTGTTCCGCCGCGAGGGGCGCCAGGTACAGCTGACCGATGTCGGCCGCCTGTACCAGCGCGAGCTGAGCGAGGCACTGGGGCGCATCCGCAGCGCCACCTTGCAGGCACTGGCCTATCAATCGGGAGTAGGCACGTTGCGCCTGGCCACCCTGCCAACCTTCGGTTCGAAATGGCTGTTGCCGAGG  >  NC_002947/5108320‑5108608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑