| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,119,834 | G→C | 10.0% | A141G (GCC→GGC) | PP_4506 ← | nitroreductase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,119,834 | 0 | G | C | 10.0% | 137.7 / 7.6 | 50 | A141G (GCC→GGC) | PP_4506 | nitroreductase family protein |
| Reads supporting (aligned to +/- strand): ref base G (24/21); new base C (2/3); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
CCAAGGCCAGGTTACTCACCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCCACCACCAC > NC_002947/5119687‑5119982 | ccAAGGCCAGGTTACTCACCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATggg > 8:118278/1‑148 (MQ=255)ccAAGGCCAGGTTACTCACCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGAGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATggg > 2:366538/1‑148 (MQ=255) cAAGGCCAGGTTACTCCCCCCAGCTACTAACGAAATCCGCCGGTTCCAGCACCGGCGCCGTCCGCGGTTCGGGCAGCGGCGTACCGACATACCGGTAGCCGCTCCGCTCCTCGTTCTCGGACCACACCACCCCCTTGTGCACATGGGc > 6:115507/1‑148 (MQ=255) ggTTACTCACCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGc < 1:366538/149‑1 (MQ=255) gTTACTCACCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGcc < 7:118278/149‑1 (MQ=255) tACTCACCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCAt > 2:109452/1‑149 (MQ=255) aCTCACCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCAt < 1:88255/148‑1 (MQ=255) aCTCACCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATg > 7:111661/1‑149 (MQ=255) cTCACCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGt < 1:244846/149‑1 (MQ=255) aCCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCAc < 3:204641/149‑1 (MQ=255) aCCACAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGCCTTCGATGCCCATGtgc < 3:239269/148‑3 (MQ=255) aaCGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCcaca > 3:112778/1‑149 (MQ=255) aCGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCacac > 1:154607/1‑149 (MQ=255) aCGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCacac > 1:368614/1‑149 (MQ=255) tCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGcc > 2:43290/1‑149 (MQ=255) gTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATccc > 3:361579/1‑149 (MQ=255) gTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGCCTTCGATGCCCATGtg < 2:359707/122‑2 (MQ=255) gTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGCCTTCGATGCCCATGtg > 1:359707/1‑121 (MQ=255) cGGCGCCGTCCGCGGTTCGGACAGCGGCGTACCGACATACAGGTAGCCGCACTGCTCCACGTACTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCAAGTCACCGGTACGCCACACCGCGCCGCTCCCCTGCGCGTgcg > 1:97181/1‑149 (MQ=255) gcgcCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGGGCgca < 5:115507/149‑1 (MQ=255) tCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGACACACCGCGCCGATCCCCTGCGCGTGCGCAGCAAt > 7:335142/1‑148 (MQ=255) ccGCGGTTCGGTCAGCCGCGTACCGTCCTACTGGTAGCCGATCAGCTCCGCGTTGTCGGCCATCCCCAACCCCTTGTGCAGATGGGAATCGATGGCCATGTCAGCGGTACGCCACACCGCGCCGCTCCCCTGCGCGTGCGCAGCAATct < 2:97181/149‑2 (MQ=255) cgcgGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAg < 4:133606/149‑1 (MQ=255) gcgGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGa < 1:43290/149‑1 (MQ=255) ggTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAAt < 3:189945/149‑1 (MQ=255) gTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTg > 6:42427/1‑149 (MQ=255) aGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTgcgc > 7:72658/1‑149 (MQ=255) cggcgTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGcgccg < 8:111661/149‑1 (MQ=255) cGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGccc > 6:345888/1‑149 (MQ=255) cGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGcc < 6:378639/148‑1 (MQ=255) cGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGcc > 5:378639/1‑148 (MQ=255) gACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCccg > 6:340075/1‑149 (MQ=255) aGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGCCTTCGATGCCCATGt > 3:327704/1‑70 (MQ=38) aGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGCCTTCGATGCCCATGt < 4:327704/70‑1 (MQ=38) gTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGgc < 8:52191/149‑1 (MQ=255) gTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGgc > 5:364476/1‑149 (MQ=255) gCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCAACGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAAAACCGAGCGCCGCACAGCCCCCCGCCCGGCgct > 8:361164/1‑149 (MQ=255) aTCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTc < 2:368614/149‑1 (MQ=255) aGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGa < 7:361164/149‑1 (MQ=255) tcctcGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACtt > 1:59053/1‑149 (MQ=255) cctcGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTg < 5:42427/149‑1 (MQ=255) ggCCAACCCCAAGCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTg < 2:82189/148‑1 (MQ=255) ggCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCtt < 4:112778/147‑1 (MQ=255) ccaaccccaaccCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAg > 1:261773/1‑149 (MQ=255) cccaaccccTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAg < 5:233358/144‑1 (MQ=255) cccaaccccTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACATTGAAg > 6:233358/1‑144 (MQ=255) caaccccTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCt < 6:364476/149‑1 (MQ=255) cccTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCaa > 3:109258/1‑149 (MQ=255) cacaTGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGgc > 5:180425/1‑104 (MQ=255) cacaTGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGgc < 6:180425/104‑1 (MQ=255) acaTGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGccac > 5:198604/1‑149 (MQ=255) tGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGccaccac > 8:19472/1‑149 (MQ=255) gggCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGaa > 2:236896/1‑124 (MQ=255) gggCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGaa < 1:236896/124‑1 (MQ=255) gCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGccac < 8:302533/143‑1 (MQ=255) gCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCcaccaccac > 1:184484/1‑149 (MQ=255) gCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCcaccaccac > 2:170959/1‑149 (MQ=255) gCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCcaccaccac > 2:307981/1‑149 (MQ=255) gCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCcaccaccac > 6:303956/1‑149 (MQ=255) gCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCAAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGccac > 7:302533/1‑143 (MQ=255) | CCAAGGCCAGGTTACTCACCCCAGCTACTAACGAAATCAGCCGTTTCCAGCACCGGCGCCGTCCGCGGTTCGGTCAGCGGCGTACCGACATACAGGTAGCCGATCAGCTCCTCGTTCTCGGCCAACCCCAACCCCTTGTGCACATGGGCATCGAAGGCCATGTCACCGGTACGCCACACCGCGCCGATCCCCTGCGCGTGCGCAGCAATCAGAATACCGTGCGCCGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCCACCACCAC > NC_002947/5119687‑5119982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |