Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,168,178 C→G 7.3% intergenic (‑299/‑205) hrpA ← / → PP_4547 ATP‑dependent helicase HrpA/glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,168,1780CG 7.3% 163.5 / 4.3 55intergenic (‑299/‑205)hrpA/PP_4547ATP‑dependent helicase HrpA/glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base C (31/20);  new base G (2/2);  total (33/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

CGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTT  >  NC_002947/5168037‑5168324
                                                                                                                                             |                                                                                                                                                  
cGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGaa                                                                                                                                             <  1:139585/149‑1 (MQ=255)
  tggtgCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAgg                                                                                                                                           <  1:341862/149‑1 (MQ=255)
  tggtgCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAgg                                                                                                                                           >  7:174269/1‑149 (MQ=255)
     tgCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCATACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGGTAGCGAAgggcg                                                                                                                                        <  4:347977/149‑4 (MQ=255)
       cTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGGTAGCGAAgggcgg                                                                                                                                       <  4:89865/148‑5 (MQ=255)
               acGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGc                                                                                                                              <  6:230595/149‑1 (MQ=255)
                 gAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCg                                                                                                                            <  2:247022/149‑1 (MQ=255)
                  aaTGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCgg                                                                                                                           <  7:291743/149‑1 (MQ=255)
                    tGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCgggg                                                                                                                         <  7:160196/149‑1 (MQ=255)
                       ttAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGaa                                                                                                                       >  3:353741/1‑148 (MQ=255)
                         aGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAAt                                                                                                                    >  3:138205/1‑149 (MQ=255)
                           ccAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATcc                                                                                                                  >  8:218950/1‑149 (MQ=255)
                           ccAAGTGCAATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATcc                                                                                                                  >  7:290952/1‑149 (MQ=255)
                                   gATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAATGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTcc                                                                                                          <  4:270754/149‑1 (MQ=255)
                                   gATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTc                                                                                                           >  2:278315/1‑148 (MQ=255)
                                    aTGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCCCCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCca                                                                                                         >  2:333920/1‑149 (MQ=255)
                                          aGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGCCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCAcatcat                                                                                                   >  8:348138/1‑149 (MQ=255)
                                            gTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATcc                                                                                                 <  6:314309/149‑1 (MQ=255)
                                               gCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTc                                                                                               <  8:290952/148‑1 (MQ=255)
                                                 cATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTACCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGCGCCGTGCCGGGGAAAATCCGCTGGTCCACATCAACCTCgac                                                                                            >  8:90869/1‑147 (MQ=255)
                                                        tGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTggg                                                                                     >  4:109063/1‑149 (MQ=255)
                                                               aTCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTtgtg                                                                              <  1:278315/149‑1 (MQ=255)
                                                                     cTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTg                                                                        >  2:174385/1‑149 (MQ=255)
                                                                          gccgcATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGt                                                                    >  5:185543/1‑148 (MQ=255)
                                                                          gccgcATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGt                                                                    >  4:73574/1‑148 (MQ=255)
                                                                            cgcATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCAc                                                                 <  6:273703/149‑1 (MQ=255)
                                                                                 gtgAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCAcgcgcg                                                            >  2:215743/1‑149 (MQ=255)
                                                                                       aTCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCATCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGGCTTGTGGCACTGCCGTCACGCGCGCCCAcc                                                      >  1:97169/1‑149 (MQ=255)
                                                                                        tCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCg                                                     >  4:356183/1‑149 (MQ=255)
                                                                                         cAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGTCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCgg                                                    >  1:117534/1‑149 (MQ=255)
                                                                                          aaaGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGa                                                   >  5:386063/1‑149 (MQ=255)
                                                                                          aaaGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGa                                                   <  8:174269/149‑1 (MQ=255)
                                                                                          aaaGGCATGACTCCCGCCCAGCCCATCGCCCCACAGTCGTCCCCCCCTACCCAACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCCCTGGTCCCCATCATCCTCGCCGTCTGGGCCTTTTGGCACGGCCGTCACGCGCGCCCACCCGa                                                   >  8:326204/1‑149 (MQ=255)
                                                                                                 tGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCg                                            <  1:333920/149‑1 (MQ=255)
                                                                                                    cTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTc                                         <  7:218950/149‑1 (MQ=255)
                                                                                                     tCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATACTCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGCCACTTCGCTCa                                        >  6:83740/1‑149 (MQ=255)
                                                                                                     tCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCGTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATGCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCCCTGCCGTCACGCCCGCCCACCGGACACTTCGCtac                                        >  3:288977/1‑147 (MQ=255)
                                                                                                       ccGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACg                                      >  3:67033/1‑149 (MQ=255)
                                                                                                        cGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGc                                     <  4:138205/149‑1 (MQ=255)
                                                                                                            cagcccaTCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGTCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCAtt                                 <  2:117534/149‑1 (MQ=255)
                                                                                                             agcccaTCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATtg                                >  8:89378/1‑149 (MQ=255)
                                                                                                              gcccaTCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATtgt                               <  3:109063/149‑1 (MQ=255)
                                                                                                               cccaTCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCGGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCGCCCGCCATtgtg                              >  7:59382/1‑149 (MQ=255)
                                                                                                                  aTCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCCCCGGACACTTCGCTCACGCCATTGTGAc                            >  5:19265/1‑148 (MQ=255)
                                                                                                                  aTCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGAcc                           <  4:353741/149‑1 (MQ=255)
                                                                                                                        ccACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAAccc                     >  5:172153/1‑149 (MQ=255)
                                                                                                                         cACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACCCTTCGCTCACGCCATTGTGACCAAACCCg                    >  2:278614/1‑149 (MQ=255)
                                                                                                                          aCCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCgg                   >  1:39106/1‑149 (MQ=255)
                                                                                                                            cgtcgtCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGTCACTTCGCTCACGCCATTGTGACCAAACCCGGcc                 >  7:216110/1‑149 (MQ=255)
                                                                                                                                 tccgggccTTCGGTAGCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCCCATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGt            >  6:233976/7‑149 (MQ=255)
                                                                                                                                 tcCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGt            >  6:229363/1‑149 (MQ=255)
                                                                                                                                    gggccTTCGGTAGCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTAGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTg         >  3:36850/4‑149 (MQ=255)
                                                                                                                                         ttCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGt    >  8:257253/1‑149 (MQ=255)
                                                                                                                                           cGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGt    <  1:278614/147‑1 (MQ=255)
                                                                                                                                            gCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGTCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGttt  <  7:326204/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
CGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTATCAAAGGCATGACTCCCGCCCAGCCCATCGCCCCACCGTCGTCCCGCCCTTCGCTACCGAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTT  >  NC_002947/5168037‑5168324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑