| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,173,244 | T→C | 9.0% | intergenic (‑285/+47) | fadD‑I ← / ← fadD‑II | long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,173,244 | 0 | T | C | 9.0% | 150.0 / 5.6 | 58 | intergenic (‑285/+47) | fadD‑I/fadD‑II | long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (26/25); new base C (2/3); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01 | |||||||||||
AGGCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTG > NC_002947/5173098‑5173390 | agGCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTg < 4:54896/149‑1 (MQ=255) cAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCa < 2:97503/149‑1 (MQ=255) aGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAg < 7:50915/149‑1 (MQ=255) gCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGa < 1:189649/149‑1 (MQ=255) cTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGc < 8:8450/149‑1 (MQ=255) ttATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCg > 7:281147/1‑149 (MQ=255) tGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAATGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACACTGCCCAATGTAGGAGCggg < 6:365329/148‑1 (MQ=38) tGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCggg < 6:243959/148‑1 (MQ=255) tGGTCAAAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACACTGCCCAATGTAGGAGCGGGt < 7:316418/149‑1 (MQ=38) gTCACAAAAATGACTCAATGAACAATACAGGTCAGTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGtt > 1:37013/1‑148 (MQ=255) aaaaaTGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCcg > 3:330902/1‑149 (MQ=255) atcccccAACGAACAACACAGCCCATCTTTACCCAAATAATCCACAATCCCCCAGATAAGCCTGTCTAGAGCCTG‑CTACACCGGTTTAAGGCATCCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGCAGCGGGTTTACCCGCgaa < 8:281147/143‑1 (MQ=16) aCTCAATCAACAATACCGGTCATTTTAAGAGTAATAATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAACGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCgaagaa < 4:317547/149‑1 (MQ=38) aaTGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGCTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTg < 1:275394/114‑1 (MQ=255) aaTGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTg > 2:275394/1‑113 (MQ=255) aaTGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGGCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGc < 1:13571/147‑1 (MQ=255) aaCAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATcc < 7:352774/148‑1 (MQ=255) aaCAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTACTAAATTGGGCAGTGTAGGAGCGGGTTTTCCCGCGAAGAAGCCATacc > 5:26627/1‑149 (MQ=255) aaTACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGGGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGt > 6:7015/1‑149 (MQ=255) tACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTa > 3:53501/1‑148 (MQ=255) aGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTAAATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCAc > 3:207036/1‑149 (MQ=255) cATTTTTATAGTAATTATCAAAAATGTCGCATTAAAGCCTGTATCGAGCGTT‑TTACAGCGTTTTACGCCATTCGCGGTTAACCCGGCCCCTACATTGGGCTGTTTCGGCGGGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCt < 6:26627/148‑1 (MQ=255) gagTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATcgcg < 5:197874/148‑1 (MQ=255) aaTTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCACCt > 2:6720/1‑149 (MQ=255) tCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATcc > 3:70450/1‑119 (MQ=255) tCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATcc < 4:70450/119‑1 (MQ=255) tCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATAGCGCAACTCccgc > 3:65175/1‑149 (MQ=255) ccAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCccgcc > 5:101487/1‑149 (MQ=255) aaaaTGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGTTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCcgccgc < 4:379838/149‑1 (MQ=255) aaaaTGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCccgccg > 2:120448/1‑148 (MQ=255) aTGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGGGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCaa > 8:177128/1‑148 (MQ=255) tGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCaaaa < 4:207036/149‑1 (MQ=255) aTAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCGCCGACAATGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATAGCGGAACAGCCGCCGCAAAATAGTGccc > 8:312252/1‑149 (MQ=38) aGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCAc < 2:37013/148‑1 (MQ=255) ggCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCg > 1:333316/1‑149 (MQ=255) gCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATAGCGCAACTCCCGCCGCAAAATCTTGCCCACCgg > 5:137651/1‑149 (MQ=255) cTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGc < 3:314968/149‑1 (MQ=255) gTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGt > 5:169690/1‑149 (MQ=255) gagCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATgg < 1:152161/149‑1 (MQ=255) agCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATggg < 6:101487/149‑1 (MQ=255) cTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCACCTACAATGGGGAGTGTAGGAGCGGGGTTAACCGCGAAGAAACCCTCCCGGTATAAAGCGAAAAGGCGCAACTCCCCCCGCAAAATCTTGCCCACCGCCGGCCTGGGCa > 8:202853/1‑148 (MQ=255) tAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGa < 4:53501/148‑1 (MQ=255) tttAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGGTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATAGCGCAACTACCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGAATCGCgcag > 3:199025/1‑149 (MQ=255) aGGCATTCGCGGGTAAACCCGCTCCGACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATAACGCGATATCGCGCAACTACCGCCGCAAAATCTTGCACACCGGCGTCAAGGGCAACGCTTAGAGCAGAAc > 4:309391/1‑148 (MQ=37) gCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGAt < 4:330902/149‑1 (MQ=255) gcgGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCtt < 6:169690/149‑1 (MQ=255) gggTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTgg < 5:7015/149‑1 (MQ=255) ggTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTggg > 3:191367/1‑149 (MQ=255) aaCCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCTTCCCGGTATCACGCTATATCGCGCAACTGCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGCTGTGCTGGGGTACt > 8:245144/1‑149 (MQ=255) aaCCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCCGCACGATGTGCTTGGGTACt > 8:245145/1‑149 (MQ=255) aCCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACtt > 7:185581/1‑149 (MQ=255) cccGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACttt > 4:2786/1‑149 (MQ=255) cccGCTCCTACACTGCCCAATGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACtt > 1:197330/1‑148 (MQ=255) cccGCTCCTACACTGCCCAATGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACtt < 2:197330/148‑1 (MQ=255) gCTCCTACACTGCCCAATGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTg > 7:275010/1‑147 (MQ=255) cTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGcc < 6:137651/148‑1 (MQ=255) tACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCgg > 5:293661/1‑149 (MQ=255) aTTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTg > 2:80069/1‑148 (MQ=255) | AGGCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTG‑TTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTG > NC_002947/5173098‑5173390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |