Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 62,080 C→T 9.3% Q109* (CAG→TAG)  PP_0053 → sulfide:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294762,0800CT 9.3% 116.1 / 2.7 43Q109* (CAG→TAG) PP_0053sulfide:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (22/17);  new base T (2/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01

TGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTCAAGCAAG  >  NC_002947/61932‑62224
                                                                                                                                                    |                                                                                                                                                
tGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCTTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCTGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAAc                                                                                                                                                  <  8:98759/149‑1 (MQ=255)
   cGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGc                                                                                                                                               >  3:343386/1‑149 (MQ=255)
      tgtgtTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGa                                                                                                                                            >  6:169917/1‑149 (MQ=255)
          ttCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTGTTCATAGCTGAtggc                                                                                                                                        <  2:185501/149‑4 (MQ=255)
          ttCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCg                                                                                                                                         >  8:320415/1‑148 (MQ=255)
           tCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTGTTCATAGCTGAtggc                                                                                                                                        <  1:356600/148‑4 (MQ=255)
                 cACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGcccc                                                                                                                                 >  8:287970/1‑149 (MQ=255)
                  aCCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGAt                                                                                                                                           >  2:345441/1‑138 (MQ=255)
                  aCCAAGCACTGCCCGCACCATCGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGAt                                                                                                                                           <  1:345441/138‑1 (MQ=255)
                     aaGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGcc                                                                                                                             >  3:75687/1‑149 (MQ=255)
                                        gCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGggcggc                                                                                                          >  3:11133/1‑149 (MQ=255)
                                             gACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTg                                                                                                     <  8:348457/149‑1 (MQ=255)
                                                     cTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTc                                                                                             >  4:54187/1‑149 (MQ=255)
                                                      tCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCt                                                                                              >  3:180406/1‑147 (MQ=255)
                                                        gcgGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCa                                                                                            <  7:320415/147‑1 (MQ=255)
                                                         cgGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCg                                                                                         <  4:75687/149‑1 (MQ=255)
                                                          ggTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCga                                                                                        <  6:390565/149‑1 (MQ=255)
                                                                       tCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCACACGCTGGACTGGGCGGCCATTGATGGGATCAGCGAGACCCTGGGCCg                                                                            >  8:337499/1‑148 (MQ=255)
                                                                         aaGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCaa                                                                         <  4:343386/149‑1 (MQ=255)
                                                                               cgGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGgtgt                                                                   <  4:11133/149‑1 (MQ=255)
                                                                                  gTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCAc                                                                >  4:23850/1‑149 (MQ=255)
                                                                                        ggATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCaa                                                          >  2:183397/1‑149 (MQ=255)
                                                                                          aTTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAAc                                                         >  6:33736/1‑148 (MQ=255)
                                                                                              gACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATc                                                     <  3:23850/148‑1 (MQ=255)
                                                                                                 ccATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGCCTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTa                                                 >  6:125127/1‑149 (MQ=255)
                                                                                                 ccATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTa                                                 >  2:274608/1‑149 (MQ=255)
                                                                                                  cATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTAc                                                <  7:287970/149‑1 (MQ=255)
                                                                                                         ccAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTgg                                         >  3:342824/1‑149 (MQ=255)
                                                                                                                gTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCTCCCt                                   <  5:330000/148‑1 (MQ=255)
                                                                                                                gTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGACCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCta                                  >  6:351391/1‑149 (MQ=255)
                                                                                                                             atgGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTg                     <  1:183397/149‑1 (MQ=255)
                                                                                                                                gTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGt                   <  5:33736/148‑1 (MQ=255)
                                                                                                                                gTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTg                  <  1:274608/149‑1 (MQ=255)
                                                                                                                                   agacgaTCAGCTGTTCATAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGCCCCTGGGCCGCAACGGTGTCCCCTCCAAATATCGCTACGACCTGGAACCCTATACCTGGGAGCTGGTGCa                >  7:275973/6‑148 (MQ=255)
                                                                                                                                    gacgaTCAGCTGTTCATAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaa               >  7:101199/5‑148 (MQ=255)
                                                                                                                                     gacATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGTGCCGCAACTGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaaaa             <  7:337499/147‑1 (MQ=255)
                                                                                                                                           aGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTg                     >  7:321823/1‑135 (MQ=255)
                                                                                                                                           aGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTg                     <  8:321823/135‑1 (MQ=255)
                                                                                                                                             cTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTcaag      >  1:181368/1‑148 (MQ=255)
                                                                                                                                              tATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTcaagc     >  3:163453/1‑148 (MQ=255)
                                                                                                                                               aTGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaaaa             <  6:11756/139‑1 (MQ=255)
                                                                                                                                               aTGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaaaa             >  5:11756/1‑139 (MQ=255)
                                                                                                                                                 gAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTcaagcaag  >  1:335043/1‑148 (MQ=255)
                                                                                                                                                   aCAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCAccc                                    <  8:27426/112‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTCAAGCAAG  >  NC_002947/61932‑62224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑