Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,212,472	C→T	6.8%	intergenic (+36/+58)	PP_4593 → / ← PP_4594	hypothetical protein/cystathionine gamma‑synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,212,472	0	C	T	6.8%	219.8 / 4.3	73	intergenic (+36/+58)	PP_4593/PP_4594	hypothetical protein/cystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base C (29/39);  new base T (2/3);  total (31/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GTAGCCGCCGAAGGCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTC  >  NC_002947/5212326‑5212620                                                                                                                                                      |                                                                                                                                                     gtAGCCGCCGAAGGCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCaa                                                                                                                                                    <  2:387889/149‑1 (MQ=255)   aGCCGCCGAAGGCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGc                                                                                                                                                  >  7:122718/1‑149 (MQ=255)          gAAGGCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTcc                                                                                                                                           <  2:4250/149‑1 (MQ=255)              gCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTaca                                                                                                                                       <  5:109434/149‑1 (MQ=255)              gCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTaca                                                                                                                                       >  6:109434/1‑149 (MQ=255)                aaCGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAcc                                                                                                                                     >  4:259936/1‑149 (MQ=255)                   ggCACCAACGACGATGCCCTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtc                                                                                                                                  <  8:134860/149‑1 (MQ=255)                   ggCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtc                                                                                                                                  <  6:91526/149‑1 (MQ=255)                    gCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtcg                                                                                                                                 <  6:275286/149‑1 (MQ=255)                       ccAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAg                                                                                                                              <  2:311516/149‑1 (MQ=255)                          acgacgAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAg                                                                                                                           <  2:193663/149‑1 (MQ=255)                             acgaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCgg                                                                                                                        <  8:62145/149‑1 (MQ=255)                               gaAGCCGTCAACCGCCTGCGCAAGGAAAGGCTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGt                                                                                                                      <  6:221824/149‑1 (MQ=255)                                   ccGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGATTAc                                                                                                                  <  2:70586/149‑1 (MQ=255)                                     gTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCTTTCATCCGCCCAAATACTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACCCCGTCGTAGGAGCGGGATTAacc                                                                                                                >  6:207171/1‑149 (MQ=255)                                      tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcg                                                                                                               >  1:44704/1‑149 (MQ=255)                                      tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcg                                                                                                               >  5:36148/1‑149 (MQ=255)                                          ccGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGa                                                                                                            >  3:3377/1‑148 (MQ=255)                                             ccTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                        <  4:270010/149‑1 (MQ=255)                                             ccTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                        <  2:346746/149‑1 (MQ=255)                                             ccTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                        <  3:302103/149‑1 (MQ=255)                                              cTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAACGTCGTAGGAGCGGGTTTACCCGCGAAAggg                                                                                                       <  7:253441/149‑1 (MQ=255)                                                 cgcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCt                                                                                                    <  3:259936/149‑1 (MQ=255)                                                  gcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGttt                                                                                                                    >  4:375329/1‑132 (MQ=255)                                                  gcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGttt                                                                                                                    <  3:375329/132‑1 (MQ=255)                                                     aGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCgg                                                                                                <  2:26010/149‑1 (MQ=255)                                                       gAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTAGAGTTGAATAAACCGGCCTATTCGCGGGCAAGCCCGCTCATACACCGTCGTAGGAGCGGTTTTACCCGCGAAAGGGCCTGCg                                                                                                 >  1:116947/1‑149 (MQ=255)                                                               gCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTcaca                                                                                        >  8:348005/1‑147 (MQ=255)                                                                  gATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGc                                                                                    >  6:94043/1‑148 (MQ=255)                                                                   aTCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGa                                                                                  >  8:10049/1‑149 (MQ=255)                                                                    tCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAc                                                                                 >  7:163522/1‑149 (MQ=255)                                                                         gACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCtttcgcg                                                                                      <  7:12284/139‑7 (MQ=38)                                                                         gACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCtttcgag                                                                                      >  8:12284/1‑133 (MQ=38)                                                                            aaGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCtt                                                                         >  8:317576/1‑149 (MQ=255)                                                                              gATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTc                                                                       <  7:317576/149‑1 (MQ=255)                                                                                   aaCGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGc                                                                  <  4:3377/149‑1 (MQ=255)                                                                                       gATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCa                                                              >  3:381043/1‑149 (MQ=255)                                                                                               atCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCa                                                                               <  5:27036/124‑1 (MQ=255)                                                                                               atCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCa                                                                               >  6:27036/1‑124 (MQ=255)                                                                                                tCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcccc                                                               >  5:286490/1‑139 (MQ=255)                                                                                                tCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcccc                                                               <  6:286490/139‑1 (MQ=255)                                                                                                  cGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGCCCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                   >  6:328020/1‑149 (MQ=255)                                                                                                  cGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                   >  6:64421/1‑149 (MQ=255)                                                                                                  cGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGATCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACTCCCCATCCAGGTCCTc                                                   <  2:63717/149‑1 (MQ=255)                                                                                                       aaaTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                              <  7:348005/149‑1 (MQ=255)                                                                                                       aaaTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                              <  2:44704/149‑1 (MQ=255)                                                                                                        aaTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGCCCAg                                             >  7:299771/1‑149 (MQ=255)                                                                                                         aTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                            <  5:207171/149‑1 (MQ=255)                                                                                                         aTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                            >  7:106176/1‑149 (MQ=255)                                                                                                         aTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                            <  1:187097/149‑1 (MQ=255)                                                                                                          tCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                            >  2:181082/1‑148 (MQ=255)                                                                                                            cTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCa                                         <  5:94043/149‑1 (MQ=255)                                                                                                                    cATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATc                                 <  4:381043/149‑1 (MQ=255)                                                                                                                             ttGAATCCACCGGCCTCTTCGCTGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGa                            <  6:36148/148‑1 (MQ=255)                                                                                                                             ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCt                               <  7:10049/145‑1 (MQ=255)                                                                                                                             ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTc                              <  5:64421/146‑1 (MQ=255)                                                                                                                             ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGAt                           <  8:299771/149‑1 (MQ=255)                                                                                                                               gAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGCCCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATcc                         >  4:209308/1‑149 (MQ=255)                                                                                                                                   ccACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCTTCGATCCCTAc                     >  6:34883/1‑149 (MQ=255)                                                                                                                                   ccACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAc                     <  2:223864/149‑1 (MQ=255)                                                                                                                                    cACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAcc                    >  5:180351/1‑149 (MQ=255)                                                                                                                                       cGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGAc                 <  8:122718/149‑1 (MQ=255)                                                                                                                                        ggccctttCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTc                              >  3:293242/7‑135 (MQ=21)                                                                                                                                        ggccctttCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTc                              <  4:293242/129‑1 (MQ=21)                                                                                                                                            tctTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGCCCAGGTCACGGTAATCCTCGATCCCTCCCGACAACCg            >  3:202184/1‑149 (MQ=255)                                                                                                                                              ttCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                      <  4:62376/121‑1 (MQ=255)                                                                                                                                              ttCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                      >  3:62376/1‑121 (MQ=255)                                                                                                                                                 gcgGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgcag         >  6:191669/1‑147 (MQ=38)                                                                                                                                                  cgGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag      <  3:169718/149‑1 (MQ=25)                                                                                                                                                  cgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGccgcag      <  2:116947/149‑1 (MQ=255)                                                                                                                                                  cgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag      <  1:181082/149‑1 (MQ=255)                                                                                                                                                   gggTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAgg     <  5:191669/149‑1 (MQ=38)                                                                                                                                                   gggCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAgg     >  1:307501/1‑149 (MQ=255)                                                                                                                                                      cAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTtc  >  1:340434/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GTAGCCGCCGAAGGCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTC  >  NC_002947/5212326‑5212620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑