Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,212,487	A→G	8.8%	intergenic (+51/+43)	PP_4593 → / ← PP_4594	hypothetical protein/cystathionine gamma‑synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,212,487	0	A	G	8.8%	219.5 / 9.3	80	intergenic (+51/+43)	PP_4593/PP_4594	hypothetical protein/cystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base A (33/39);  new base G (4/3);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

GCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATC  >  NC_002947/5212339‑5212635                                                                                                                                                        |                                                                                                                                                     gCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTaca                                                                                                                                                      <  5:109434/149‑1 (MQ=255) gCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTaca                                                                                                                                                      >  6:109434/1‑149 (MQ=255)   aaCGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAcc                                                                                                                                                    >  4:259936/1‑149 (MQ=255)      ggCACCAACGACGATGCCCTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtc                                                                                                                                                 <  8:134860/149‑1 (MQ=255)      ggCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtc                                                                                                                                                 <  6:91526/149‑1 (MQ=255)       gCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtcg                                                                                                                                                <  6:275286/149‑1 (MQ=255)          ccAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAg                                                                                                                                             <  2:311516/149‑1 (MQ=255)             acgacgAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAg                                                                                                                                          <  2:193663/149‑1 (MQ=255)                acgaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCgg                                                                                                                                       <  8:62145/149‑1 (MQ=255)                  gaAGCCGTCAACCGCCTGCGCAAGGAAAGGCTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGt                                                                                                                                     <  6:221824/149‑1 (MQ=255)                      ccGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGATTAc                                                                                                                                 <  2:70586/149‑1 (MQ=255)                        gTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCTTTCATCCGCCCAAATACTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACCCCGTCGTAGGAGCGGGATTAacc                                                                                                                               >  6:207171/1‑149 (MQ=255)                         tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcg                                                                                                                              >  1:44704/1‑149 (MQ=255)                         tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcg                                                                                                                              >  5:36148/1‑149 (MQ=255)                             ccGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGa                                                                                                                           >  3:3377/1‑148 (MQ=255)                                ccTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                       <  4:270010/149‑1 (MQ=255)                                ccTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                       <  3:302103/149‑1 (MQ=255)                                ccTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                       <  2:346746/149‑1 (MQ=255)                                 cTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAACGTCGTAGGAGCGGGTTTACCCGCGAAAggg                                                                                                                      <  7:253441/149‑1 (MQ=255)                                    cgcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCt                                                                                                                   <  3:259936/149‑1 (MQ=255)                                     gcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGttt                                                                                                                                   >  4:375329/1‑132 (MQ=255)                                     gcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGttt                                                                                                                                   <  3:375329/132‑1 (MQ=255)                                        aGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCgg                                                                                                               <  2:26010/149‑1 (MQ=255)                                          gAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTAGAGTTGAATAAACCGGCCTATTCGCGGGCAAGCCCGCTCATACACCGTCGTAGGAGCGGTTTTACCCGCGAAAGGGCCTGCg                                                                                                                >  1:116947/1‑149 (MQ=255)                                                  gCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTcaca                                                                                                       >  8:348005/1‑147 (MQ=255)                                                     gATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGc                                                                                                   >  6:94043/1‑148 (MQ=255)                                                      aTCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGa                                                                                                 >  8:10049/1‑149 (MQ=255)                                                       tCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAc                                                                                                >  7:163522/1‑149 (MQ=255)                                                            gACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCtttcgcg                                                                                                     <  7:12284/139‑7 (MQ=38)                                                            gACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCtttcgag                                                                                                     >  8:12284/1‑133 (MQ=38)                                                               aaGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCtt                                                                                        >  8:317576/1‑149 (MQ=255)                                                                 gATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTc                                                                                      <  7:317576/149‑1 (MQ=255)                                                                      aaCGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGc                                                                                 <  4:3377/149‑1 (MQ=255)                                                                          gATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCa                                                                             >  3:381043/1‑149 (MQ=255)                                                                                  atCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCa                                                                                              >  6:27036/1‑124 (MQ=255)                                                                                  atCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCa                                                                                              <  5:27036/124‑1 (MQ=255)                                                                                   tCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcccc                                                                              <  6:286490/139‑1 (MQ=255)                                                                                   tCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcccc                                                                              >  5:286490/1‑139 (MQ=255)                                                                                     cGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGCCCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                                  >  6:328020/1‑149 (MQ=255)                                                                                     cGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                                  >  6:64421/1‑149 (MQ=255)                                                                                     cGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGATCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACTCCCCATCCAGGTCCTc                                                                  <  2:63717/149‑1 (MQ=255)                                                                                          aaaTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                                             <  7:348005/149‑1 (MQ=255)                                                                                          aaaTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                                             <  2:44704/149‑1 (MQ=255)                                                                                           aaTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGCCCAg                                                            >  7:299771/1‑149 (MQ=255)                                                                                            aTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                                           <  5:207171/149‑1 (MQ=255)                                                                                            aTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                                           >  7:106176/1‑149 (MQ=255)                                                                                            aTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                                           <  1:187097/149‑1 (MQ=255)                                                                                             tCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                                           >  2:181082/1‑148 (MQ=255)                                                                                               cTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCa                                                        <  5:94043/149‑1 (MQ=255)                                                                                                       cATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATc                                                <  4:381043/149‑1 (MQ=255)                                                                                                                ttGAATCCACCGGCCTCTTCGCTGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGa                                           <  6:36148/148‑1 (MQ=255)                                                                                                                ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCt                                              <  7:10049/145‑1 (MQ=255)                                                                                                                ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTc                                             <  5:64421/146‑1 (MQ=255)                                                                                                                ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGAt                                          <  8:299771/149‑1 (MQ=255)                                                                                                                  gAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGCCCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATcc                                        >  4:209308/1‑149 (MQ=255)                                                                                                                      ccACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCTTCGATCCCTAc                                    >  6:34883/1‑149 (MQ=255)                                                                                                                      ccACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAc                                    <  2:223864/149‑1 (MQ=255)                                                                                                                       cACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAcc                                   >  5:180351/1‑149 (MQ=255)                                                                                                                          cGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGAc                                <  8:122718/149‑1 (MQ=255)                                                                                                                           ggccctttCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTc                                             >  3:293242/7‑135 (MQ=21)                                                                                                                           ggccctttCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTc                                             <  4:293242/129‑1 (MQ=21)                                                                                                                               tctTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGCCCAGGTCACGGTAATCCTCGATCCCTCCCGACAACCg                           >  3:202184/1‑149 (MQ=255)                                                                                                                                 ttCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                                     >  3:62376/1‑121 (MQ=255)                                                                                                                                 ttCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                                     <  4:62376/121‑1 (MQ=255)                                                                                                                                    gcgGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgcag                        >  6:191669/1‑147 (MQ=38)                                                                                                                                     cgGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag                     <  3:169718/149‑1 (MQ=25)                                                                                                                                     cgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGccgcag                     <  2:116947/149‑1 (MQ=255)                                                                                                                                     cgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag                     <  1:181082/149‑1 (MQ=255)                                                                                                                                      gggTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAgg                    <  5:191669/149‑1 (MQ=38)                                                                                                                                      gggCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAgg                    >  1:307501/1‑149 (MQ=255)                                                                                                                                         cAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTtc                 >  1:340434/1‑149 (MQ=255)                                                                                                                                            gCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATc                                         <  7:271154/122‑1 (MQ=255)                                                                                                                                            gCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATc                                         >  8:271154/1‑122 (MQ=255)                                                                                                                                            gCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCg              <  2:307501/149‑1 (MQ=255)                                                                                                                                            gCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCg              >  4:74997/1‑149 (MQ=255)                                                                                                                                              ccGCTCCTACGACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCt            >  1:159084/1‑149 (MQ=255)                                                                                                                                              ccGCTCCTACGACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCt            >  2:131609/1‑149 (MQ=255)                                                                                                                                              ccGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCt            >  2:253011/1‑149 (MQ=255)                                                                                                                                                    cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAc                                    >  2:235410/1‑119 (MQ=255)                                                                                                                                                    cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAc                                    <  1:235410/119‑1 (MQ=255)                                                                                                                                                    cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATccc      >  8:203536/1‑149 (MQ=255)                                                                                                                                                    cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATccc      >  5:189180/1‑149 (MQ=255)                                                                                                                                                       cacCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCa    >  8:287545/1‑148 (MQ=255)                                                                                                                                                        acCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATc  >  7:184835/1‑149 (MQ=255)                                                                                                                                                        |                                                                                                                                                     GCAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATC  >  NC_002947/5212339‑5212635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑