Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,212,504	T→C	7.1%	intergenic (+68/+26)	PP_4593 → / ← PP_4594	hypothetical protein/cystathionine gamma‑synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,212,504	0	T	C	7.1%	209.5 / 4.6	74	intergenic (+68/+26)	PP_4593/PP_4594	hypothetical protein/cystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base T (36/31);  new base C (2/3);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

GAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATCACCCCTTTCTGCTCTG  >  NC_002947/5212356‑5212651                                                                                                                                                        |                                                                                                                                                    gaAGCCGTCAACCGCCTGCGCAAGGAAAGGCTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGt                                                                                                                                                     <  6:221824/149‑1 (MQ=255)     ccGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGATTAc                                                                                                                                                 <  2:70586/149‑1 (MQ=255)       gTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCTTTCATCCGCCCAAATACTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACCCCGTCGTAGGAGCGGGATTAacc                                                                                                                                               >  6:207171/1‑149 (MQ=255)        tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcg                                                                                                                                              >  5:36148/1‑149 (MQ=255)        tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcg                                                                                                                                              >  1:44704/1‑149 (MQ=255)            ccGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGa                                                                                                                                           >  3:3377/1‑148 (MQ=255)               ccTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                                       <  3:302103/149‑1 (MQ=255)               ccTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                                       <  4:270010/149‑1 (MQ=255)               ccTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                                       <  2:346746/149‑1 (MQ=255)                cTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAACGTCGTAGGAGCGGGTTTACCCGCGAAAggg                                                                                                                                      <  7:253441/149‑1 (MQ=255)                   cgcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCt                                                                                                                                   <  3:259936/149‑1 (MQ=255)                    gcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGttt                                                                                                                                                   <  3:375329/132‑1 (MQ=255)                    gcAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGttt                                                                                                                                                   >  4:375329/1‑132 (MQ=255)                       aGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCgg                                                                                                                               <  2:26010/149‑1 (MQ=255)                         gAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTAGAGTTGAATAAACCGGCCTATTCGCGGGCAAGCCCGCTCATACACCGTCGTAGGAGCGGTTTTACCCGCGAAAGGGCCTGCg                                                                                                                                >  1:116947/1‑149 (MQ=255)                                 gCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTcaca                                                                                                                       >  8:348005/1‑147 (MQ=255)                                    gATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGc                                                                                                                   >  6:94043/1‑148 (MQ=255)                                     aTCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGa                                                                                                                 >  8:10049/1‑149 (MQ=255)                                      tCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAc                                                                                                                >  7:163522/1‑149 (MQ=255)                                           gACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCtttcgcg                                                                                                                     <  7:12284/139‑7 (MQ=38)                                           gACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCtttcgag                                                                                                                     >  8:12284/1‑133 (MQ=38)                                              aaGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCtt                                                                                                        >  8:317576/1‑149 (MQ=255)                                                gATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTc                                                                                                      <  7:317576/149‑1 (MQ=255)                                                     aaCGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGc                                                                                                 <  4:3377/149‑1 (MQ=255)                                                         gATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCa                                                                                             >  3:381043/1‑149 (MQ=255)                                                                 atCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCa                                                                                                              >  6:27036/1‑124 (MQ=255)                                                                 atCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCa                                                                                                              <  5:27036/124‑1 (MQ=255)                                                                  tCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcccc                                                                                              <  6:286490/139‑1 (MQ=255)                                                                  tCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcccc                                                                                              >  5:286490/1‑139 (MQ=255)                                                                    cGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGCCCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                                                  >  6:328020/1‑149 (MQ=255)                                                                    cGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                                                  >  6:64421/1‑149 (MQ=255)                                                                    cGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGATCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACTCCCCATCCAGGTCCTc                                                                                  <  2:63717/149‑1 (MQ=255)                                                                         aaaTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                                                             <  7:348005/149‑1 (MQ=255)                                                                         aaaTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                                                             <  2:44704/149‑1 (MQ=255)                                                                          aaTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGCCCAg                                                                            >  7:299771/1‑149 (MQ=255)                                                                           aTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                                                           <  5:207171/149‑1 (MQ=255)                                                                           aTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                                                           <  1:187097/149‑1 (MQ=255)                                                                           aTCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                                                           >  7:106176/1‑149 (MQ=255)                                                                            tCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAgg                                                                           >  2:181082/1‑148 (MQ=255)                                                                              cTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCa                                                                        <  5:94043/149‑1 (MQ=255)                                                                                      cATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATc                                                                <  4:381043/149‑1 (MQ=255)                                                                                               ttGAATCCACCGGCCTCTTCGCTGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGa                                                           <  6:36148/148‑1 (MQ=255)                                                                                               ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCt                                                              <  7:10049/145‑1 (MQ=255)                                                                                               ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTc                                                             <  5:64421/146‑1 (MQ=255)                                                                                               ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGAt                                                          <  8:299771/149‑1 (MQ=255)                                                                                                 gAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGCCCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATcc                                                        >  4:209308/1‑149 (MQ=255)                                                                                                     ccACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCTTCGATCCCTAc                                                    >  6:34883/1‑149 (MQ=255)                                                                                                     ccACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAc                                                    <  2:223864/149‑1 (MQ=255)                                                                                                      cACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAcc                                                   >  5:180351/1‑149 (MQ=255)                                                                                                         cGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGAc                                                <  8:122718/149‑1 (MQ=255)                                                                                                          ggccctttCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTc                                                             <  4:293242/129‑1 (MQ=21)                                                                                                          ggccctttCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTc                                                             >  3:293242/7‑135 (MQ=21)                                                                                                              tctTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGCCCAGGTCACGGTAATCCTCGATCCCTCCCGACAACCg                                           >  3:202184/1‑149 (MQ=255)                                                                                                                ttCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                                                     <  4:62376/121‑1 (MQ=255)                                                                                                                ttCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                                                     >  3:62376/1‑121 (MQ=255)                                                                                                                   gcgGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgcag                                        >  6:191669/1‑147 (MQ=38)                                                                                                                    cgGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag                                     <  3:169718/149‑1 (MQ=25)                                                                                                                    cgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGccgcag                                     <  2:116947/149‑1 (MQ=255)                                                                                                                    cgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag                                     <  1:181082/149‑1 (MQ=255)                                                                                                                     gggTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAgg                                    <  5:191669/149‑1 (MQ=38)                                                                                                                     gggCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAgg                                    >  1:307501/1‑149 (MQ=255)                                                                                                                        cAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTtc                                 >  1:340434/1‑149 (MQ=255)                                                                                                                           gCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATc                                                         >  8:271154/1‑122 (MQ=255)                                                                                                                           gCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATc                                                         <  7:271154/122‑1 (MQ=255)                                                                                                                           gCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCg                              <  2:307501/149‑1 (MQ=255)                                                                                                                           gCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCg                              >  4:74997/1‑149 (MQ=255)                                                                                                                             ccGCTCCTACGACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCt                            >  2:131609/1‑149 (MQ=255)                                                                                                                             ccGCTCCTACGACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCt                            >  1:159084/1‑149 (MQ=255)                                                                                                                             ccGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCt                            >  2:253011/1‑149 (MQ=255)                                                                                                                                   cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAc                                                    >  2:235410/1‑119 (MQ=255)                                                                                                                                   cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAc                                                    <  1:235410/119‑1 (MQ=255)                                                                                                                                   cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATccc                      >  8:203536/1‑149 (MQ=255)                                                                                                                                   cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATccc                      >  5:189180/1‑149 (MQ=255)                                                                                                                                      cacCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCa                    >  8:287545/1‑148 (MQ=255)                                                                                                                                       acCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATc                  >  7:184835/1‑149 (MQ=255)                                                                                                                                           tcgtAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATCAccc              <  6:180351/149‑1 (MQ=255)                                                                                                                                                       gTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATCACCCCTTTCTGCTCTg  >  4:188091/1‑149 (MQ=255)                                                                                                                                                        |                                                                                                                                                    GAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTA‑‑‑TTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCCCATCACCCCTTTCTGCTCTG  >  NC_002947/5212356‑5212651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑