Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,232,054 C→G 8.8% A214A (GCG→GCC)  PP_4610 ← PepSY‑associated TM helix domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,232,0540CG 8.8% 195.4 / 5.4 64A214A (GCG→GCC) PP_4610PepSY‑associated TM helix domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (31/27);  new base G (2/4);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGGCTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTGAA  >  NC_002947/5231908‑5232200
                                                                                                                                                  |                                                                                                                                                  
cccGGCTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCccgc                                                                                                                                                  >  8:254983/1‑149 (MQ=255)
 ccGGCTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCcggcg                                                                                                                                                 >  5:182738/1‑148 (MQ=255)
         aGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCAc                                                                                                                                         <  7:117611/149‑1 (MQ=255)
          gCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCTGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCatg                                                                                                                                        <  4:102259/149‑3 (MQ=255)
          gCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCAc                                                                                                                                         >  2:317479/1‑148 (MQ=255)
           cTCCTGCACCTTGGCGTAGAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGGCGTTGAAGTACTTGTCGGGGTCATTGCCGTAGCTGGCCGTGATGCCGGCGGGCATCatgg                                                                                                                                       <  3:283689/149‑4 (MQ=255)
           cTCCTGCACCTTGGCGTACCGGCTCGGCAGCGCTCCCCGCGGCGTGGCCACCTGGGCCGCCGTGGGCGCAGCGTTGCGGCCAAACAGGTCGTGGAAGTACTGGTCGGGGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCatgg                                                                                                                                       <  5:103880/149‑4 (MQ=255)
           cTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCGGCGGGCATCatgg                                                                                                                                       <  6:182738/149‑4 (MQ=255)
           cTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCa                                                                                                                                       >  3:255854/1‑149 (MQ=255)
            tCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCAt                                                                                                                                      >  4:159204/1‑149 (MQ=255)
              cTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCAt                                                                                                                                      <  7:211214/147‑1 (MQ=255)
              cTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATg                                                                                                                                     >  7:156032/1‑148 (MQ=255)
                 cACCTTGGCGTACGGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCAGCGTTGCGGCCAAACAGGTCGTTGGAGTGGTTGTCGGTGTCATTGGCGGAGCTGGCCATGATGGCGGCCGGCATCACCaggga                                                                                                                                   >  8:366384/1‑143 (MQ=255)
                  aCCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACAt                                                                                                                                <  6:69373/149‑1 (MQ=255)
                   ccTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATg                                                                                                                               <  7:366384/149‑1 (MQ=255)
                               ggCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTTGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAgg                                                                                                                   <  5:99606/149‑1 (MQ=255)
                               ggCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAgg                                                                                                                   >  7:128501/1‑149 (MQ=255)
                                gCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGgc                                                                                                                  >  6:28927/1‑149 (MQ=255)
                                    ggCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGgctgc                                                                                                               <  5:39181/148‑1 (MQ=255)
                                     gCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGctgctg                                                                                                             >  3:328033/1‑149 (MQ=255)
                                       aGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTa                                                                                                           <  4:36261/149‑1 (MQ=255)
                                           cTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTg                                                                                                       <  4:328033/149‑1 (MQ=255)
                                           cTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCACGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCt                                                                                                        >  6:372422/1‑148 (MQ=255)
                                              ccAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCTTTTAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCGCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGat                                                                                                     <  7:32227/148‑1 (MQ=255)
                                              ccAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGa                                                                                                      <  4:255854/147‑1 (MQ=255)
                                               cAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGatca                                                                                                   >  5:330284/1‑149 (MQ=255)
                                               cAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGATGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGatca                                                                                                   >  8:301824/1‑149 (MQ=255)
                                                aGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGatcat                                                                                                  >  6:207536/1‑149 (MQ=255)
                                                 gcggcgTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATg                                                                                                                               <  8:248346/119‑1 (MQ=255)
                                                 gcggcgTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATg                                                                                                                               >  7:248346/1‑119 (MQ=255)
                                                 gcggcgTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGatcatc                                                                                                 <  3:32472/149‑1 (MQ=255)
                                                   ggcgTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAg                                                                                               <  3:159204/149‑1 (MQ=255)
                                                    gcgTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAgg                                                                                              >  3:194276/1‑149 (MQ=255)
                                                    gcgTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAgg                                                                                              <  6:312597/149‑1 (MQ=255)
                                                                  gccgccTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGcagcac                                                                                <  7:26166/149‑1 (MQ=255)
                                                                   ccgccTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGcagcac                                                                                >  1:225896/1‑148 (MQ=255)
                                                                   ccgccTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGcagcac                                                                                >  4:377130/1‑148 (MQ=255)
                                                                            gcgcATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGAtcatca                                                                                                >  2:343760/1‑123 (MQ=255)
                                                                            gcgcATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGAtcatca                                                                                                <  1:343760/123‑1 (MQ=255)
                                                                                aTCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCa                                                                                                                     >  5:295703/1‑98 (MQ=255)
                                                                                aTCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCa                                                                                                                     <  6:295703/98‑1 (MQ=255)
                                                                                   gTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATAACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATGATCAGGTGAAACGGCAGCACACGCACGCCGATGGCAt                                                               >  8:244484/1‑149 (MQ=255)
                                                                                       cGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATtgtg                                                           <  7:301824/149‑1 (MQ=255)
                                                                                         gCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGAGAAACGGCAGCACCAGCACGCCGAAGGCAATGTGcc                                                         >  6:137183/1‑149 (MQ=255)
                                                                                             aaCAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTc                                                     <  1:317479/149‑1 (MQ=255)
                                                                                                  gTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTccagc                                                 >  5:269056/1‑148 (MQ=255)
                                                                                                         aaGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACg                                         >  7:215763/1‑149 (MQ=255)
                                                                                                               ttGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGccc                                   >  8:212138/1‑149 (MQ=255)
                                                                                                                  tCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTAGATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGACCGGTGccccgt                                >  4:253416/1‑147 (MQ=255)
                                                                                                                     gtgtCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGcc                             <  7:212138/149‑1 (MQ=255)
                                                                                                                     gtgtCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGc                              >  8:239856/1‑148 (MQ=255)
                                                                                                                          aTTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCCGCACGCCGCTGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGc                        >  7:166793/1‑149 (MQ=255)
                                                                                                                          aTTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGc                        <  6:330284/149‑1 (MQ=255)
                                                                                                                             gCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCgg                      <  8:128501/148‑1 (MQ=255)
                                                                                                                               cGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGa                     >  7:197423/1‑147 (MQ=255)
                                                                                                                               cGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGa                     <  8:197423/147‑1 (MQ=255)
                                                                                                                                 tAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGt                 >  4:187140/1‑149 (MQ=255)
                                                                                                                                       atggtgatgCCGGCGGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTgaagaa           >  8:190371/5‑149 (MQ=255)
                                                                                                                                        ccATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGGCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAAc          <  8:156032/149‑1 (MQ=255)
                                                                                                                                          atgatgCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTc        <  3:377130/149‑1 (MQ=255)
                                                                                                                                          atgatgCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTc        >  1:332907/1‑149 (MQ=255)
                                                                                                                                           tgatgCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACTCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTcc       <  5:137183/149‑1 (MQ=255)
                                                                                                                                            gatgCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCt      >  8:205494/1‑149 (MQ=255)
                                                                                                                                               gCCCGCCGGCATCCCCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTg    >  5:217652/1‑148 (MQ=255)
                                                                                                                                               gCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGCACGCTGCCCCTTGCCAGGGCGGAAg                   >  4:81884/1‑133 (MQ=255)
                                                                                                                                               gCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAg                   <  3:81884/133‑1 (MQ=255)
                                                                                                                                                cccGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCATGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAATGTGAAGAACTCCTTGaa  <  5:372422/149‑1 (MQ=255)
                                                                                                                                                cccGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTGa   <  2:332907/148‑1 (MQ=255)
                                                                                                                                                 ccgccgGCATAACCATGTACATGAACAGAACCAGGCTGAAGTACCTGATCATCAGGTGAAACGCAAGCACCAGCACGCCGATGGCATTGTGCACGTCCAGCCAGGAACGCTGCCCCTTGGCAGGGGGGAAGGTGAAGAGCTCCTTg    >  6:240932/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCCGGCTGCAGCTCCTGCACCTTGGCGTACAGGCTCGGCAGCGCTACCAGCGGCGTGGCCACCTGGGCCGCCTTGGGCGCATCGTTGCGGCCAAACAGGTCGTTGAAGTACTTGTCGGTGTCATTGCCGTAGCTGGCCATGATGCCCGCCGGCATCACCATGTACATGAACAGCACCAGGCTGCTGTAGCTGATCATCAGGTGAAACGGCAGCACCAGCACGCCGATGGCATTGTGCCCGTCCAGCCAGGAACGCTGCCCCTTGCCAGGGCGGAAGGTGAAGAACTCCTTGAA  >  NC_002947/5231908‑5232200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑