| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,233,549 | G→A | 10.3% | G138D (GGC→GAC) | PP_4611 → | ECF family RNA polymerase sigma‑70 factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,233,549 | 0 | G | A | 10.3% | 234.9 / 12.2 | 88 | G138D (GGC→GAC) | PP_4611 | ECF family RNA polymerase sigma‑70 factor |
| Reads supporting (aligned to +/- strand): ref base G (37/41); new base A (5/4); total (42/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TGCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATTGGTTG > NC_002947/5233402‑5233696 | tGCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCAAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGc > 5:243730/1‑149 (MQ=255) gCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGcc > 6:123042/1‑149 (MQ=255) aGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTgg < 5:337224/149‑1 (MQ=255) aGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTgg > 2:388058/1‑149 (MQ=255) gcgcTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGCCGTCGACCTggg < 6:103058/149‑1 (MQ=255) gcTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGc > 5:120769/1‑148 (MQ=255) gcTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCATGCTGGACCA‑GGTCGACGGCCTGGGc > 6:379065/1‑148 (MQ=255) cTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGATCAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCa < 1:138039/149‑1 (MQ=255) tCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTg < 8:222920/143‑1 (MQ=255) tCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTg > 7:222920/1‑143 (MQ=255) cacaGGGCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGc < 3:307474/149‑1 (MQ=255) tCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAAtc > 1:239103/1‑149 (MQ=255) tCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGACCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAAtc > 1:123404/1‑149 (MQ=255) cccGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAAtcg > 4:26598/1‑149 (MQ=255) aaGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAAtcgctcgc < 5:219157/149‑1 (MQ=255) gTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCag > 5:310371/1‑128 (MQ=255) gTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCag < 6:310371/129‑2 (MQ=255) gcgcCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGGGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTggg > 1:85762/1‑149 (MQ=255) ccGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCAt < 3:167929/149‑1 (MQ=255) gTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCAtt < 1:280777/149‑1 (MQ=255) gTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCAttt < 2:222476/149‑1 (MQ=255) gTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGGCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCAttt < 3:25122/149‑1 (MQ=255) gAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCt > 4:305759/1‑149 (MQ=255) ggAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTTGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTgg < 5:379065/149‑1 (MQ=255) aGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCa < 2:286717/135‑1 (MQ=255) aGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACg < 2:239103/149‑1 (MQ=255) aaGGCTACCTTCTTCCAGACATTGCTAGCGCCCGACCACTTGCTGGACGGCCTTCCCCCGATGGACAAACGCGCCTTCCTGCTGGCCCA‑GGTCGCCGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGt < 2:197338/149‑1 (MQ=255) gATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAgc > 7:290648/1‑149 (MQ=255) gATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAgc > 2:248678/1‑149 (MQ=255) atcTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCt < 2:97695/148‑1 (MQ=255) atcTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCt < 4:154883/148‑1 (MQ=255) cTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTAcc > 3:164605/1‑149 (MQ=255) tCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCt > 2:27798/1‑148 (MQ=255) cGATACATTGCTAGCGCTCTACCACTTGCTGGACGGCCTGCCACCTTTGGTCAAACGCGCCTTCCTGCTGGCCAA‑GGTCGACGCCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTAc < 1:388058/145‑1 (MQ=255) cGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTAc < 1:279383/145‑1 (MQ=255) cGAGACATTGCTAGCGCTCGACCACTTACTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTg > 1:10574/1‑148 (MQ=255) agaCATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGaa > 7:29517/1‑148 (MQ=255) aCATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAAc > 5:275900/1‑147 (MQ=255) cATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAg < 2:123404/149‑1 (MQ=255) aTTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAgg > 2:357717/1‑149 (MQ=255) ttGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGc < 8:290648/149‑1 (MQ=255) gCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAg > 5:359794/1‑149 (MQ=255) tAGCGCTCGACCACTTGCTGGGCGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGc > 7:177978/1‑148 (MQ=255) cTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTc < 5:147224/120‑1 (MQ=255) cTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTc > 6:147224/1‑120 (MQ=255) gACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATgcgc < 3:26598/147‑1 (MQ=255) gACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgctg > 8:13314/1‑149 (MQ=255) aCCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGCCGTCGACCTGGGCCagca < 1:123345/76‑4 (MQ=37) aCCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGCCGTCGACCTGGGCCagca > 2:123345/1‑73 (MQ=37) ttGCTGGGCGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACtt < 8:177978/149‑1 (MQ=255) ttGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACtt > 7:239788/1‑149 (MQ=255) ttGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACtt > 6:150271/1‑149 (MQ=255) gCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGACCTGGGCATTTCGCTGGCCACGGTCAAGCGCGACCTGAACAAGGCAGCCCTGCGCGGCTACTTcg > 6:227958/1‑149 (MQ=255) gCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTcg > 1:377819/1‑149 (MQ=255) gCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTc < 2:172414/148‑1 (MQ=255) cTGGACGGCCTTCCACCGTGGGTCAACCGCGCCTTCCTGCTGGCCCA‑GGTCGCCGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTcg < 5:227958/148‑1 (MQ=255) tGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTcgcg > 2:13933/1‑149 (MQ=255) gACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTcgcg < 2:10574/147‑1 (MQ=255) gACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCt < 6:120769/149‑1 (MQ=255) cGGCCTTCCACCGTTGGTCAAACGCGGCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCTCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt < 8:230250/149‑1 (MQ=255) cGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt < 4:133071/149‑1 (MQ=255) cGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt < 2:377819/149‑1 (MQ=255) cGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt < 4:164605/149‑1 (MQ=255) ggCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt > 5:6149/1‑148 (MQ=255) ccTTCCACCGTTGGTCTAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCt < 4:320963/90‑1 (MQ=255) cTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAAc > 7:192420/1‑149 (MQ=255) aCCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGAGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTc > 1:309602/1‑149 (MQ=255) ggTCAAACGCGCCATCCTGCTGGCCCA‑GGTCGACGGCATGGGCCAAGGCGAAATCGCTCGCGAACTGTGCATTTCGCTGGCCACGGTCAACCGGTACCAGAACAAGGCAGCCATGCGCTGCTACTTAGCGCTGAGAACGCCTCGTTTTc > 4:7251/1‑149 (MQ=255) gTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTc < 3:305759/148‑1 (MQ=255) cgcCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCtggt < 5:123042/149‑1 (MQ=255) tCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCg > 5:274669/1‑148 (MQ=255) ccTGCTGGTCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgctgc < 7:308630/106‑1 (MQ=255) ccTGCTGGTCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgctgc > 8:308630/1‑106 (MQ=255) ccttgGCCCA‑GGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGaa > 1:25521/4‑148 (MQ=255) cttgGCCCA‑GGCCGTTGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCCCGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGaa > 2:50656/3‑147 (MQ=255) cttgGCCCA‑GGCCGTCGACCTGGGCCAGGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgct > 7:267978/3‑101 (MQ=38) cttgGCCCA‑GGCCGTCGACCTGGGCCAGGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgct < 8:267978/99‑1 (MQ=38) cttgGCCCA‑GGCCGTCGACCTGGACCAGGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgct < 6:106719/99‑1 (MQ=255) cttgGCCCA‑GGCCGTCGACCTGGACCAGGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgct > 5:106719/3‑101 (MQ=255) gCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGcc < 3:343647/149‑1 (MQ=255) gCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGc < 6:6149/148‑1 (MQ=255) gCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGc < 6:359794/148‑1 (MQ=255) ccA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGt < 8:192420/149‑1 (MQ=255) ccA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGt > 5:61141/1‑149 (MQ=255) ccA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGt > 5:282810/1‑149 (MQ=255) ggTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCa < 6:243730/149‑1 (MQ=255) tCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCAtt < 6:275900/149‑1 (MQ=255) cGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATTGGt < 8:239788/148‑1 (MQ=255) ggCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGGTGGCCACGGTCAAGCGCTACCTGAACAAGGCTGCGATGCGCTGCGACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTTGCGGAACAGGCCGTGCATTGGTTg < 3:7251/149‑1 (MQ=255) | TGCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCA‑GGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATTGGTTG > NC_002947/5233402‑5233696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |