Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,239,889	T→C	7.6%	P142P (CCA→CCG)	ldh ←	leucine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,239,889	0	T	C	7.6%	181.5 / 4.7	67	P142P (CCA→CCG)	ldh	leucine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (29/32);  new base C (2/3);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.47e-01

CGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCT  >  NC_002947/5239742‑5240031                                                                                                                                                     |                                                                                                                                               cgcgCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCAtgtg                                                                                                                                               >  5:19402/1‑149 (MQ=255)      ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGAt                                                                                                                                          <  8:14843/149‑1 (MQ=255)      ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGAt                                                                                                                                          >  5:32441/1‑149 (MQ=255)      ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGAt                                                                                                                                          >  3:1662/1‑149 (MQ=255)       cACCGGATGCAATTGCTCCGCCAGGGCATAACCAACATTGCG‑CATCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGGCCAGCCGGAACGAGGTTGGGGCACGTATGCCCGCCAACACCCCCATCGCCGCTTGTGGCGATg                                                                                                                                         <  1:219294/149‑1 (MQ=255)        aCCGCATGCAATTGCTCCGCCAGTGCATAACCAACATTGCC‑GAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCATCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGCGGCGATgg                                                                                                                                        <  4:27249/149‑1 (MQ=255)        aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATgg                                                                                                                                        <  1:91572/149‑1 (MQ=255)        aCCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGCGGCGATgg                                                                                                                                        <  8:116765/149‑1 (MQ=255)         ccGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACGCCTGGACCGCTACCCGGAAGCCTCTCAGGTCATCGCTGCCCAGCCGGATCGTGGTTGTGGCACGTATGCTCGCGAACACCCCCATCGCCGCATGTGGCGATggg                                                                                                                                       <  5:147935/149‑1 (MQ=255)          cGCATGTAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGCGGCGATgggg                                                                                                                                      <  2:92340/149‑2 (MQ=255)            cATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCg                                                                                                                                    >  1:112634/1‑149 (MQ=255)            cATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCg                                                                                                                                    >  3:131348/1‑149 (MQ=255)                  aTTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGAt                                                                                                                              >  4:136791/1‑149 (MQ=255)                   ttGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATg                                                                                                                             <  8:355239/149‑1 (MQ=255)                   ttGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATg                                                                                                                             <  3:120924/149‑1 (MQ=255)                       tCCGCCAGGGCATAACCAACATTGCCGCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAg                                                                                                                          >  1:201211/1‑149 (MQ=255)                       tCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCAtgt                                                                                                                                                >  7:295200/1‑126 (MQ=255)                       tCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCAtgt                                                                                                                                                <  8:295200/126‑1 (MQ=255)                        ccGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGCCCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGGTGTGGCACGTATGCCCGCGAACTCCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAgtg                                                                                                                        <  5:306838/149‑1 (MQ=255)                         cGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAgtg                                                                                                                        <  7:51599/148‑1 (MQ=255)                         cGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAgtg                                                                                                                        <  6:193600/148‑1 (MQ=255)                            cAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGc                                                                                                                    <  8:170572/149‑1 (MQ=255)                              gggCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTg                                                                                                                  >  7:148153/1‑149 (MQ=255)                               ggCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGAt                                                                                                                                          <  8:270950/124‑1 (MQ=255)                               ggCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGAt                                                                                                                                          >  7:270950/1‑124 (MQ=255)                               ggCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTgg                                                                                                                 <  2:112634/149‑1 (MQ=255)                                 cATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTAATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTc                                                                                                               <  6:171568/149‑1 (MQ=255)                                      ccAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTa                                                                                                          <  3:92315/149‑1 (MQ=255)                                        aaCATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAg                                                                                                                          <  1:259504/131‑1 (MQ=255)                                        aaCATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAg                                                                                                                          >  2:259504/1‑131 (MQ=255)                                        aaCATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAgg                                                                                                        >  6:253449/1‑149 (MQ=255)                                        aaCATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAgg                                                                                                        >  8:203791/1‑149 (MQ=255)                                             tGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTg                                                                                                   >  4:220482/1‑149 (MQ=255)                                              gCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTg                                                                                                   <  4:104802/148‑1 (MQ=255)                                               cc‑cAACCCCTGGACCGCTACCCTCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTACGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGc                                                                                                  <  5:174445/148‑1 (MQ=255)                                                c‑cAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCtg                                                                                                >  6:236822/1‑149 (MQ=255)                                                    aCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                             <  6:19402/149‑1 (MQ=255)                                                    aCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                             <  3:136791/149‑1 (MQ=255)                                                     ccccTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCTATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTggg                                                                                            <  4:1662/149‑1 (MQ=255)                                                     ccccTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTggg                                                                                            >  7:159965/1‑149 (MQ=255)                                                           gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATg                                                                                       <  3:156175/148‑1 (MQ=255)                                                           gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATg                                                                                       <  5:253449/148‑1 (MQ=255)                                                           gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATg                                                                                       <  8:215772/148‑1 (MQ=255)                                                                  aCCCGCAAGCCTCTCAGGTCATCGATGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGACCACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCATTGGTGCTGGTCACGTAGGGCGTGCTGTCGGCAATGCAGTCCAt                                                                               >  4:215557/1‑149 (MQ=255)                                                                      gCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGc                                                                                                                    >  6:48775/1‑108 (MQ=255)                                                                        aaGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCaa                                                                         <  3:172589/149‑1 (MQ=255)                                                                            ctctCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTc                                                                     >  4:156531/1‑149 (MQ=255)                                                                                 aGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAg                                                                  <  5:236822/147‑1 (MQ=255)                                                                                 aGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGt                                                                >  1:19781/1‑149 (MQ=255)                                                                                              ccAGCTGGCAGGAGGTTGTGTCACGTCTGCCCGCGAACACCCCCATGGCCGCATGTGTCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACg                                                   <  7:166230/149‑1 (MQ=255)                                                                                               ccgCCGGACCGAGGGTGTGGCACGTATGCCCGGCATCACCCCCATCGCCGCCTGGGGCGATGGCTCGCCCGATTCAGGGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACgg                                                  <  4:217876/147‑1 (MQ=255)                                                                                               cAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACgg                                                  >  8:33414/1‑149 (MQ=255)                                                                                               cAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCAAAGGTCGAGGTGCCACTGTCCACgg                                                  >  1:163492/1‑149 (MQ=255)                                                                                                  ccGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                               <  7:203791/149‑1 (MQ=255)                                                                                                     gAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatga                                            <  3:220482/149‑1 (MQ=255)                                                                                                                  gCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCAt                                <  4:131348/148‑1 (MQ=255)                                                                                                                    aCGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGc                              >  8:302917/1‑148 (MQ=255)                                                                                                                            ccGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGCTGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCCCTGTCCACGGCGAGGATGAAGCGCCCATGCAAGGTATc                      >  3:192577/1‑148 (MQ=255)                                                                                                                              gcgAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGAt                   >  5:322084/1‑149 (MQ=255)                                                                                                                                  acacCCCCATCGCCTCTTGTGGCGTTGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAg               <  5:58089/149‑1 (MQ=255)                                                                                                                                  acacCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAg               >  7:316345/1‑149 (MQ=255)                                                                                                                                      ccccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCCCGTAGGGCGTGCTGGGGGCAATGCAGTCCATGTCCAAGGTCGCGGTGCCACTGTCCACGGCGATGATGAAGCGCCCCTGCAAGGTATCGATGAAGCGGc           >  7:361004/1‑149 (MQ=255)                                                                                                                                      ccccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCAGGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGc           >  4:13096/1‑149 (MQ=255)                                                                                                                                      ccccATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGc           >  2:83676/1‑149 (MQ=255)                                                                                                                                       cccATCGCCACATGCGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTACGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGc           >  7:137712/1‑148 (MQ=255)                                                                                                                                         cATCGCCACATGCGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa         >  4:18938/1‑148 (MQ=255)                                                                                                                                          aTCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa         >  8:233258/1‑147 (MQ=255)                                                                                                                                              ccgcATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGcc   <  3:156531/149‑1 (MQ=255)                                                                                                                                              ccgcATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCAACGGCGATGATGAAGCGCCGATGCAAGGTATCGATGAAGCGGCCAAAGGcc   >  5:261340/1‑149 (MQ=255)                                                                                                                                               cgcATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCt  <  2:19781/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               CGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCC‑CAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCT  >  NC_002947/5239742‑5240031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑