Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,241,709 A→G 7.1% L55L (TTG→CTG)  hmgC ← maleylacetoacetate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,241,7090AG 7.1% 288.3 / 8.7 100L55L (TTG→CTG) hmgCmaleylacetoacetate isomerase
Reads supporting (aligned to +/- strand):  ref base A (42/50);  new base G (4/3);  total (47/53)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

Only 100 of 104 total aligned reads displayed.

ATGTCGCAACCAATGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTGGAACGGTAATA  >  NC_002947/5241561‑5241856
                                                                                                                                                    |                                                                                                                                                        
aTGTCGCAACCAATGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCaa                                                                                                                                                          >  2:177727/1‑149 (MQ=255)
 tGTCGCAACCAATGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAg                                                                                                                                                         <  4:105429/149‑1 (MQ=255)
   tCGCAACCAATGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGc                                                                                                                                                       <  3:239937/149‑1 (MQ=255)
       aaCCAATGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑Gt                                                                                                                                                  <  5:206327/149‑1 (MQ=255)
          cAATGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTAcg                                                                                                                                               >  3:94243/1‑149 (MQ=255)
           aaTGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCTCCAGCACCTCACCCCCGTCGGTGCGCACGGCCG‑GTCcgc                                                                                                                                              >  2:239868/1‑149 (MQ=255)
           aaTGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTAcgc                                                                                                                                              >  3:116616/1‑149 (MQ=255)
           aaTGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTAcgc                                                                                                                                              >  7:127522/1‑149 (MQ=255)
           aaTGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGAACCTCCCCACCGTCGGTGCGCAAGGCCG‑GTCcgc                                                                                                                                              >  4:4721/1‑149 (MQ=255)
            atgatgGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAGGGCCgcgtaccg                                                                                                                                              >  7:285685/1‑142 (MQ=255)
             tgatgGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCgg                                                                                                                                            >  2:207690/1‑149 (MQ=255)
                tgGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGccc                                                                                                                                         >  2:132559/1‑149 (MQ=255)
                   ccgccACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAGGGCCGCGTAC‑CGGCCTTGc                                                                                                                                      >  7:254779/1‑149 (MQ=255)
                   ccgccACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAGGGCCGCGTAC‑CGGCCTTGc                                                                                                                                      <  8:254779/149‑1 (MQ=255)
                      ccACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAGGGCCGCGTAC‑CGGCCTTGCgca                                                                                                                                   <  7:176491/149‑3 (MQ=255)
                      ccACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAGGGCCGCGTAC‑CGGCCTTGCgca                                                                                                                                   <  8:285685/149‑3 (MQ=255)
                      ccACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCggg                                                                                                                                   <  7:224105/149‑1 (MQ=255)
                        aCCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGtt                                                                                                                                 >  4:148833/1‑149 (MQ=255)
                         ccccccGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTg                                                                                                                                >  3:122609/1‑149 (MQ=255)
                          cccccGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGa                                                                                                                               <  4:84902/149‑1 (MQ=255)
                            cccGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACg                                                                                                                             >  2:121186/1‑149 (MQ=255)
                            cccGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACg                                                                                                                             <  4:94243/149‑1 (MQ=255)
                            cccGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACg                                                                                                                             >  8:41498/1‑149 (MQ=255)
                            cccGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGAAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCgggaactcg                                                                                                                             >  1:332028/1‑143 (MQ=255)
                                aaCCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTa                                                                                                                         <  3:314173/149‑1 (MQ=255)
                                 aCCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTa                                                                                                                         >  5:39053/1‑148 (MQ=255)
                                   ccTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACa                                                                                                                       >  7:130386/1‑148 (MQ=255)
                                         ccGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACAAGCACCTCACCACCGTCGGTGCGCAAGGCGG‑GTACGCGGCCCTGCGGGTTGCCGGCGACATAGccgt                                                                                                                >  4:36486/1‑147 (MQ=255)
                                          cGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTc                                                                                                               >  6:235941/1‑149 (MQ=255)
                                           gTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCg                                                                                                              <  4:215857/149‑1 (MQ=255)
                                               cTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCAc                                                                                                          >  7:7080/1‑149 (MQ=255)
                                                  ggcggTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACgc                                                                                                        <  4:194444/148‑1 (MQ=255)
                                                   gcggTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACgctg                                                                                                      <  8:127522/149‑1 (MQ=255)
                                                   gcggTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACgctg                                                                                                      <  2:184785/149‑1 (MQ=255)
                                                   gcggTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACgctg                                                                                                      <  2:21057/149‑1 (MQ=255)
                                                    cggTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACgctgc                                                                                                     <  6:39053/149‑1 (MQ=255)
                                                     ggTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACgctgct                                                                                                    >  2:289783/1‑149 (MQ=255)
                                                           cGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑t                                                                                          >  8:214594/1‑149 (MQ=255)
                                                                 caACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑Tgcag                                                                                      >  4:36654/1‑147 (MQ=255)
                                                                   aCGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAgg                                                                                  <  1:132559/149‑1 (MQ=255)
                                                                   aCGCAGGCTGTGGATAAAACTCTTCCAGGTACTCGATGATCGCAGGCGACTGCACCAGAACCTCACAACCGTCGGTGCGCAAGACCG‑CTACGCGGCCCACAGGGATAACGCCTACATAGCCATCGCCACGCTGCTAA‑CCC‑‑‑TGAACCAgg                                                                                  >  7:194567/1‑149 (MQ=255)
                                                                             tgGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGc                                                                         <  5:318724/148‑1 (MQ=255)
                                                                               gATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAgg                                                                      <  5:200538/149‑1 (MQ=255)
                                                                               gATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAgg                                                                      <  8:130386/149‑1 (MQ=255)
                                                                               gATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAgg                                                                      >  5:170942/1‑149 (MQ=255)
                                                                               gATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAgg                                                                      >  7:219555/1‑149 (MQ=255)
                                                                                aTAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAgg                                                                      >  1:21151/1‑148 (MQ=255)
                                                                                 tAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGa                                                                    >  6:135843/1‑149 (MQ=255)
                                                                                  aaaCCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGAc                                                                   <  3:36654/149‑1 (MQ=255)
                                                                                   aaCCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGAc                                                                   <  4:300889/148‑1 (MQ=255)
                                                                                     ccTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTgg                                                                >  4:367233/1‑149 (MQ=255)
                                                                                       tctTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGTGACTGGt                                                               >  1:326234/1‑148 (MQ=255)
                                                                                           ccAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTaa                                                             <  1:177727/146‑1 (MQ=255)
                                                                                           ccAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTaa                                                             <  7:214594/146‑1 (MQ=255)
                                                                                           ccAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACAACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTAA‑CCC‑‑‑TGAAGCAGGTTGACCGGCAGGGACTGGTAagc                                                           >  8:264276/1‑148 (MQ=255)
                                                                                            cAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAagcc                                                          <  3:4721/148‑1 (MQ=255)
                                                                                               gTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAagccagcc                                                      >  2:209404/1‑149 (MQ=255)
                                                                                               gTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAagccagcc                                                      <  1:370708/149‑1 (MQ=255)
                                                                                                 aCTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTg                                                                               <  8:195943/122‑1 (MQ=255)
                                                                                                 aCTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTg                                                                               >  7:195943/1‑122 (MQ=255)
                                                                                                     gatgatCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCtt                                                  <  4:179996/147‑1 (MQ=255)
                                                                                                     gatgatCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTc                                                 <  4:122609/148‑1 (MQ=255)
                                                                                                     gatgatCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTc                                                 <  5:235941/148‑1 (MQ=255)
                                                                                                     gatgatCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCa                                                <  1:289783/149‑1 (MQ=255)
                                                                                                     gatgatCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCa                                                <  5:135843/149‑1 (MQ=255)
                                                                                                         atCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCCGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGc                                            <  7:155370/149‑1 (MQ=255)
                                                                                                          tCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGcc                                           <  3:36486/149‑1 (MQ=255)
                                                                                                            gCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAg                                         <  1:207690/149‑1 (MQ=255)
                                                                                                             ccGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGc                                            <  5:210103/145‑1 (MQ=255)
                                                                                                             ccGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGc                                            >  6:210103/1‑145 (MQ=255)
                                                                                                             ccGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAgg                                        >  5:52285/1‑149 (MQ=255)
                                                                                                              cGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCACCCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAgg                                        <  1:102283/149‑1 (MQ=255)
                                                                                                                gCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGc                                      >  1:70402/1‑148 (MQ=255)
                                                                                                                gCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGc                                      >  4:263325/1‑148 (MQ=255)
                                                                                                                   aCTGCCCCCCCACCTCACCCCCGCCGGTGCGCAAGGCCG‑CTACGCGGCCCCGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑CGCAGCAGCTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATg                                  <  4:289015/149‑1 (MQ=255)
                                                                                                                    cTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCACGGCAATgc                                 <  1:198675/149‑1 (MQ=255)
                                                                                                                     tGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGa                                                                    <  2:214247/113‑1 (MQ=255)
                                                                                                                       cACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAggg                                       >  7:234746/1‑140 (MQ=255)
                                                                                                                       cACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAggg                                       <  8:234746/140‑1 (MQ=255)
                                                                                                                       cACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCa                              >  3:90428/1‑149 (MQ=255)
                                                                                                                        aCCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCAc                             >  7:153032/1‑149 (MQ=255)
                                                                                                                            gCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCTTTCAGTGCCAGGGCAATGCGCACCCgg                         <  1:209404/149‑1 (MQ=255)
                                                                                                                               ccTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAg                      <  2:326234/149‑1 (MQ=255)
                                                                                                                                  caccacCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGAc                   <  7:176540/149‑1 (MQ=255)
                                                                                                                                   accaccGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGcc                                           <  1:181443/124‑1 (MQ=255)
                                                                                                                                   accaccGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGcc                                           >  2:181443/1‑124 (MQ=255)
                                                                                                                                    ccaccGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCCTCTTACAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGa                    <  1:121186/149‑1 (MQ=255)
                                                                                                                                     caccGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTAGATAGCCCTCGCCACGCTTCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGGCATCCCCTTGAGTGCCAGGGCAATGCGCACGCGGTAGGACGAg                <  8:194567/149‑1 (MQ=255)
                                                                                                                                     caccGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAg                <  3:310021/149‑1 (MQ=255)
                                                                                                                                       ccGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCGCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGt              <  4:116616/149‑1 (MQ=255)
                                                                                                                                        cGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTg             <  2:332028/149‑1 (MQ=255)
                                                                                                                                         gTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTgg            <  2:70402/149‑1 (MQ=255)
                                                                                                                                              tGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTGGAACgg       >  3:325784/1‑149 (MQ=255)
                                                                                                                                              cccgcAGGGCCGCGTAC‑CGGCCTTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTGGAACgg       >  4:366255/3‑149 (MQ=255)
                                                                                                                                               ccgcAGGGCCGCGTAC‑CGGCCTTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTGGAACgg       >  5:19237/2‑148 (MQ=255)
                                                                                                                                                cgcAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTGGAACGGt      >  6:35632/1‑148 (MQ=255)
                                                                                                                                                 gcAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑CGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTGGAACgg       <  6:209728/146‑1 (MQ=255)
                                                                                                                                                 gcAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑CGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTGGAACgg       >  5:209728/1‑146 (MQ=255)
                                                                                                                                                   aaGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTGGAACGGTaa    >  3:338239/1‑147 (MQ=255)
                                                                                                                                                   aaGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTGGAACGGTAATa  <  5:35632/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                        
ATGTCGCAACCAATGATGGCCGCCACCCCCCGAACCCTGGCCCGTGCCTCGGCGGTGGCCGGCAGCAACGCAGGCTGTGGATAAACCTCTTCCAGGTACTCGATGATCGCCGGCGACTGCACCAGCACCTCACCACCGTCGGTGCGCAAGGCCG‑GTACGCGGCCCTGCGGGTTGACGGCTACATAGCCCTCGCCACGCTGCTCA‑CCC‑‑‑TGCAGCAGGTTGACCGGCAGGGACTGGTAAGCCAGCCCCTTCAGTGCCAGGGCAATGCGCACCCGGTAGGACGAGGTGGAACGGTAATA  >  NC_002947/5241561‑5241856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑