BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,297,241	A→T	5.7%	V41E (GTG→GAG)	TpbB ←	diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,297,241	0	A	T	5.7%	344.2 / 5.2	115	V41E (GTG→GAG)	TpbB	diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base A (50/57); new base T (2/5); total (53/62)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

CTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGGCGCCGCGA  >  NC_002947/5297093‑5297385

cTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGCGCa                                                                                                                                                  >  4:36949/1‑149 (MQ=255)

    gCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGcc                                                                                                                                              >  8:343591/1‑149 (MQ=255)

      cACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCca                                                                                                                                            <  7:219801/149‑1 (MQ=255)

        cttcttCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCTGGGCGTGCTCGCCctgc                                                                                                                                          <  8:42039/149‑1 (MQ=255)

           cttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGTGCTCGCCctgcgt                                                                                                                                        <  7:360679/148‑3 (MQ=255)

           cttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCcagca                                                                                                                                         <  7:233393/147‑1 (MQ=255)

            ttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCCAACACCACAGCCCCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACCCCCAGCAcgg                                                                                                                                      >  6:130587/1‑149 (MQ=255)

              cGGTACTGGGAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAgg                                                                                                                                       <  6:75375/146‑1 (MQ=255)

              cGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAgg                                                                                                                                       <  2:46803/146‑1 (MQ=255)

              cGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGt                                                                                                                                     <  3:298016/148‑1 (MQ=255)

                 tACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                                                                                 <  2:22719/149‑1 (MQ=255)

                 tACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                                                                                 <  2:15260/149‑1 (MQ=255)

                   cTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGTCGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                                                                                 <  1:116672/149‑1 (MQ=255)

                    tGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTGATGGTTGGCATAGCCACGCAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                                                                                 >  7:22783/1‑146 (MQ=255)

                     ggCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                                                                                 >  8:342916/1‑145 (MQ=255)

                      gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgaga                                                                                                                             >  8:62667/1‑148 (MQ=255)

                       cAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATa                                                                                                                           <  6:279929/149‑1 (MQ=255)

                        aaTCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATAc                                                                                                                          >  6:360925/1‑149 (MQ=255)

                        aaTCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGAGCGAGCG‑‑GGCGATCAACTGCAGGGTTTGGTTGGCATAGACACGCAGGGCGCGCACGCCCCGCAGGGGGAGGGAGATAc                                                                                                                          >  8:156324/1‑149 (MQ=255)

                          tCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATAccc                                                                                                                        <  6:183640/149‑1 (MQ=255)

                           cAACCCCAGCGATTCCTTGGCGGCGGCGCTCTCATAGATCACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATAAACAGAAGGTTAACGTTCGCAAAGACACGCAGGGCGAGCACGCCCAGCTGCGTGAGTGAGATACACg                                                                                                                       >  1:345548/1‑149 (MQ=255)

                              cGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCa                                                                                                                    <  6:237956/149‑1 (MQ=255)

                               gCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAAGTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAg                                                                                                                   <  5:130587/149‑1 (MQ=255)

                                ccAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAgg                                                                                                                  >  2:196718/1‑149 (MQ=255)

                                     gATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAAc                                                                                                             <  4:175724/149‑1 (MQ=255)

                                       ttCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACgg                                                                                                           <  4:90931/149‑1 (MQ=255)

                                           ttGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGgcaag                                                                                                       >  3:380899/1‑149 (MQ=255)

                                                         gTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCt                                                                                           >  1:132968/1‑147 (MQ=255)

                                                          tcctCGAACACCACCGCCGCGTCTACGGTGTAGGTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTGGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTg                                                                                          <  8:22783/144‑1 (MQ=255)

                                                          tCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAg                                                                                        >  4:125170/1‑149 (MQ=255)

                                                            aTCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGt                                                                                      >  6:107147/1‑149 (MQ=255)

                                                            aTCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGt                                                                                      >  5:75214/1‑149 (MQ=255)

                                                            aTCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGt                                                                                      >  5:125402/1‑149 (MQ=255)

                                                             tCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTg                                                                                     <  5:390084/149‑1 (MQ=255)

                                                             tCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTg                                                                                     <  5:177703/149‑1 (MQ=255)

                                                               gAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGc                                                                                   >  1:244831/1‑149 (MQ=255)

                                                                aaCACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCa                                                                                                                    >  1:137579/1‑115 (MQ=255)

                                                                aaCACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCa                                                                                                                    <  2:137579/115‑1 (MQ=255)

                                                                aaCACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGc                                                                                   >  2:150429/1‑148 (MQ=255)

                                                                 acacCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCa                                                                                 >  2:288916/1‑149 (MQ=255)

                                                                     cacaGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAgg                                                                                                                                       >  3:125001/1‑91 (MQ=255)

                                                                     cacaGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAgg                                                                                                                                       <  4:125001/91‑1 (MQ=255)

                                                                     cacaGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGc                                                                             >  3:276106/1‑149 (MQ=255)

                                                                      acaGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGcc                                                                            >  8:161071/1‑149 (MQ=255)

                                                                      acaGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGcc                                                                            <  5:360925/149‑1 (MQ=255)

                                                                      acaGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGcc                                                                            >  3:347199/1‑149 (MQ=255)

                                                                       caGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGccc                                                                           <  6:125402/149‑1 (MQ=255)

                                                                          ccGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCt                                                                                           >  5:348329/1‑130 (MQ=255)

                                                                          ccGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCt                                                                                           <  6:348329/130‑1 (MQ=255)

                                                                          ccGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGc                                                                        <  7:268916/149‑1 (MQ=255)

                                                                            gCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCAc                                                                      >  7:122003/1‑149 (MQ=255)

                                                                             cTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCAcc                                                                     <  4:347199/149‑1 (MQ=255)

                                                                                cTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGa                                                                   <  1:25278/148‑1 (MQ=255)

                                                                                  aCGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAgcg                                                                >  8:45342/1‑149 (MQ=255)

                                                                                   cGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAgc                                                                 <  1:183275/147‑1 (MQ=255)

                                                                                   cGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAg                                                                  <  2:244831/146‑1 (MQ=255)

                                                                                    ggTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCa                                                              <  5:107147/149‑1 (MQ=255)

                                                                                     gtgtAGTTGATCGTGCT‑‑GTCTCTCAACTGCAGGTTATGGTTGGCATAGACACGCATGGCGAGCAAGCCCAGCAGTGTGAGGGAGATACCCTCCAGGCAAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCat                                                             <  1:350655/149‑2 (MQ=255)

                                                                                        caGTTGATCGCCCG‑‑CTCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGt                                                          >  6:88824/2‑149 (MQ=255)

                                                                                          gTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCg                                                        >  1:282077/1‑149 (MQ=255)

                                                                                           ttGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCgg                                                       <  7:342916/149‑1 (MQ=255)

                                                                                            tGATCGAGTG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCTAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCTAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCgg                                                       <  5:287269/148‑1 (MQ=255)

                                                                                            tGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCggg                                                      >  1:93185/1‑149 (MQ=255)

                                                                                             gATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCggg                                                      <  7:128160/148‑1 (MQ=255)

                                                                                             gATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgc                                                     >  5:345884/1‑149 (MQ=255)

                                                                                             gATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgc                                                     >  2:48884/1‑149 (MQ=255)

                                                                                              aTCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgc                                                     <  2:282077/148‑1 (MQ=255)

                                                                                               tCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgcgc                                                   <  2:93185/149‑1 (MQ=255)

                                                                                                  aGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACg                                                >  8:289539/1‑149 (MQ=255)

                                                                                                          cGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGc                                          <  4:276106/149‑1 (MQ=255)

                                                                                                           gATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACgg                                               <  2:62861/143‑1 (MQ=255)

                                                                                                             tCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTg                                       >  7:192255/1‑149 (MQ=255)

                                                                                                             tCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTg                                       >  8:174546/1‑149 (MQ=255)

                                                                                                             tCAACTGCAGGTTATGGGCGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTg                                       <  3:92525/149‑1 (MQ=255)

                                                                                                              cAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGc                                      <  7:343591/149‑1 (MQ=255)

                                                                                                               aaCTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCg                                     <  6:9732/149‑1 (MQ=255)

                                                                                                               aaCTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCg                                     <  7:62667/149‑1 (MQ=255)

                                                                                                                  tGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGt                                  >  8:364895/1‑149 (MQ=255)

                                                                                                                       gTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCt                             >  5:270271/1‑149 (MQ=255)

                                                                                                                         tATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCtt                            >  2:14827/1‑148 (MQ=255)

                                                                                                                           tGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCa                          <  6:75214/148‑1 (MQ=255)

                                                                                                                           tGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCAt                         <  7:364895/149‑1 (MQ=255)

                                                                                                                            ggTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATg                        >  7:292677/1‑149 (MQ=255)

                                                                                                                            ggTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATg                        >  8:129495/1‑149 (MQ=255)

                                                                                                                             gTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATgg                       <  7:6780/149‑1 (MQ=255)

                                                                                                                             gTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATg                        <  2:132968/148‑1 (MQ=255)

                                                                                                                                ggCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGgct                     <  1:288916/148‑1 (MQ=255)

                                                                                                                                     aGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCg               >  6:127912/1‑149 (MQ=255)

                                                                                                                                        cctgctgggCGTGCTCGCCCTGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGccc            <  1:225372/143‑1 (MQ=255)

                                                                                                                                         ccGCAGGGCGAGCCCGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCg           >  6:293155/2‑149 (MQ=255)

                                                                                                                                          tgctgggCGTGCTCGCCCTGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCtt                            >  3:34947/5‑131 (MQ=255)

                                                                                                                                          tgctgggCGTGCTCGCCCTGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCgg          >  2:204193/5‑149 (MQ=255)

                                                                                                                                          cGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCgg          <  3:36949/149‑1 (MQ=255)

                                                                                                                                          cGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCgg          >  3:122967/1‑149 (MQ=255)

                                                                                                                                           gCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGgc         <  6:345884/149‑1 (MQ=255)

                                                                                                                                             aGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGgcgc       >  6:367712/1‑149 (MQ=255)

                                                                                                                                              gggCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGGcgcc      <  1:14827/149‑1 (MQ=255)

                                                                                                                                               ggCGAGCTCGCCCAGCAGGGTGAGGGAGATAGCGGCCAGGCCAAGGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCAGCGAGGGCAGGGTCGGGCGGACGGTACGGTTGCGGGTCGGGTTCATGGCTGCTCCGCCCGGcgccg     <  2:345548/149‑1 (MQ=255)

                                                                                                                                                gCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAg                                                                                        >  1:332320/1‑65 (MQ=255)

                                                                                                                                                  gAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGGCGCCGCGa  <  7:289539/149‑1 (MQ=255)

CTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCG‑‑GGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGTACGCTTGCGGGTCGGCTTCATGGCTGCTCCGCCCGGCGCCGCGA  >  NC_002947/5297093‑5297385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`