Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,390,431	T→G	7.5%	intergenic (+520/‑202)	PP_4738 → / → PP_mr56	hypothetical protein/C4 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,390,431	0	T	G	7.5%	182.7 / 2.3	63	intergenic (+520/‑202)	PP_4738/PP_mr56	hypothetical protein/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (30/27);  new base G (3/2);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAACTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGT  >  NC_002947/5390286‑5390577                                                                                                                                                  |                                                                                                                                                    acaACTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCGACCAAATCCAAAGAAGAAGACGCCCTCgg                                                                                                                                                  <  5:159561/149‑1 (MQ=255) acaACTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCgg                                                                                                                                                  >  6:40521/1‑149 (MQ=255)   aaCTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCGAGGGc                                                                                                                                                >  8:73353/1‑149 (MQ=255)   aaCTCATCCAAAGCACCATGCCCGTCGGGCCGCTGCGGATTAAATCAGTAGACACGTCTAAGAATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCGGCGGGc                                                                                                                                                >  6:355745/1‑149 (MQ=255)     cTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGt                                                                                                                                              >  1:148562/1‑149 (MQ=255)      tCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCGACGGGCGTc                                                                                                                                             >  5:161898/1‑149 (MQ=255)        aTCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAACCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTc                                                                                                                                             <  2:101256/147‑1 (MQ=255)          ccAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑tttt                                                                                                                                        >  2:288086/1‑149 (MQ=255)          ccAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGATTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑tttt                                                                                                                                        >  4:255830/1‑149 (MQ=255)            aaaGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑tttttt                                                                                                                                      >  8:34164/1‑149 (MQ=255)               gCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCGAGGGCGTc‑ttcttcttt                                                                                                                                   <  7:73353/149‑5 (MQ=255)                       cccGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCGAGGGCGTC‑tt                                                                                                                                          <  7:166388/134‑1 (MQ=255)                       cccGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCGAGGGCGTC‑tt                                                                                                                                          >  8:166388/1‑134 (MQ=255)                         cGTCGGGCGGCTGCGGATTAAATCAGTTGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑ttt                                                                                                                                         <  6:40560/133‑1 (MQ=255)                              ggcggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCCCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGgc                                                                                                                    >  4:95203/1‑149 (MQ=255)                               gcggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGgcg                                                                                                                   <  7:21205/149‑1 (MQ=255)                                   gtGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGc                                                                                                               >  5:246416/2‑149 (MQ=255)                                     gCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCgg                                                                                                             <  3:70252/149‑1 (MQ=255)                                      cGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCgg                                                                                                             <  5:45400/148‑1 (MQ=255)                                        gATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTc                                                                                                          <  6:326766/149‑1 (MQ=255)                                                cAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATc                                                                                                  >  2:33110/1‑149 (MQ=255)                                                 aGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCGTGGAATTTGGGCGCTGCGGGTCGAAAAATc                                                                                                  <  1:33110/148‑1 (MQ=255)                                                   tAGAGACTGCTAAGCATGTAGTACCGCCAGCGGAGTACTGGCGGACGGGGGTTCAACTCCGCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGTAATTTGGGCGCTGCGGGTCGAAAAATCGGa                                                                                               <  8:80865/149‑1 (MQ=255)                                                        acGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCgg                                                                                                             <  7:261450/130‑1 (MQ=255)                                                        acGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCgg                                                                                                             >  8:261450/1‑130 (MQ=255)                                                        acGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGAC‑GGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑GTTTTTTGTGCCTGGAATTTGGTCGCTGCGGGTCGAAAAATCCGATTTAAc                                                                                         >  6:228339/1‑149 (MQ=255)                                                         cGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAc                                                                                         <  5:123393/149‑1 (MQ=255)                                                                   tGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTg                                                                               <  4:212318/149‑1 (MQ=255)                                                                     tagtaCCGACAGCTGAGTACTGGCGGACGGGGGTTCAAATCCCGCCGTCTCCACCAAATCCAAAGAAGAAGACGCCGTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTTTGCGCTTCGGTTAGAAAAATAGGATTTAACCCCTTTCTTGt                                                                              <  2:59018/148‑1 (MQ=255)                                                                     tagacccGACAGCCGAGTACTGGCGGACGTGGGTTCAACTCCCCCCGGCTCCACCAAATCCAAAGAAGACGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCTGGTCGAAAAATCGGATTTAACCCCTTTCTTGTa                                                                             <  8:203742/144‑1 (MQ=255)                                                                       gtaCAGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCACCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTCTAgg                                                                           <  5:355745/149‑1 (MQ=255)                                                                        taCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAggg                                                                          >  4:280078/1‑149 (MQ=255)                                                                          ccGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCCCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGc                                                                         <  3:95203/148‑1 (MQ=255)                                                                               aggggAGTACTGGCGGACTGGGGTTCATATACGCCCGTCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTGTGCCTGGAATTTGGGCGCTGGGGGTCGAAATATCGTATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                    <  8:215859/146‑1 (MQ=255)                                                                                 cGGAGTACTGGCGGCCGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGGC‑TTTTTTTGTGCCTGGATTTTGGGCGCTGCGGGTCGAAAGATCGGATTTAACCCCTTTCTTGTAGGGCGTTTccc                                                                  >  5:312517/1‑148 (MQ=255)                                                                                    aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCa                                                                 >  7:172572/1‑146 (MQ=255)                                                                                           gCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATAct                                                         <  1:288086/147‑1 (MQ=255)                                                                                               aCGGGGGTTTAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATAGGATTTAACCCCGTTCTTGTAGGGCGTTTCGCAAAGAGActgcctgc                                                   >  4:242261/1‑149 (MQ=255)                                                                                                 gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTTCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTgcgc                                                 >  4:168882/1‑149 (MQ=255)                                                                                                 gggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGGGCCTGGAATTTGGGCGCGGCGGGTCGCAAAATCGGATTTAACCCCTGTATTGTAGGGCGTTTCCCCAAGATAAGGCCTgcgc                                                 >  5:196576/1‑149 (MQ=255)                                                                                                   gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCGGGAATTTGGGCGCTGCGGGTCGAAAAATAGGATTTAACCCCGTTCTTGTAGGGCGTTTGCCAAAGATACTGCCAGCGCCt                                               >  2:308790/1‑149 (MQ=255)                                                                                                         aaaTCCCCCCGGCTCCACCAGATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGCTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTc                                         >  8:63121/1‑149 (MQ=255)                                                                                                          aaTCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTcc                                        >  7:271589/1‑149 (MQ=255)                                                                                                               ccccGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGc                                   >  4:277870/1‑149 (MQ=255)                                                                                                                       ccaccaAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttt                           >  5:32985/1‑149 (MQ=255)                                                                                                                         acaaaaTCCAAAGAAGAAGACGCCCTCGGGCGTC‑GTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCACTTTCTTGTAGGGCGTGTCCCAACTATACTGCCTGCGCCTGTGTTCCGTTGCGTTCAtgtta                         >  6:383461/4‑148 (MQ=255)                                                                                                                          ccaAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                         >  8:143224/1‑148 (MQ=255)                                                                                                                               tCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAggg                   <  6:32985/149‑1 (MQ=255)                                                                                                                               tCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAggg                   <  3:255830/149‑1 (MQ=255)                                                                                                                                 cAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGt                  <  6:161898/148‑1 (MQ=255)                                                                                                                                 cAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGAGTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGAGGTCATTTTTTCAGGGtt                 >  2:74829/1‑149 (MQ=255)                                                                                                                                  aaaGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgc                                                       <  1:164551/110‑1 (MQ=255)                                                                                                                                  aaaGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgc                                                       >  2:164551/1‑110 (MQ=255)                                                                                                                                  aaaGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGttt                >  8:38364/1‑149 (MQ=255)                                                                                                                                  aaaGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTGTCCGTTGCGGGCATTTTTTCAGGGtgt                >  8:252668/1‑147 (MQ=255)                                                                                                                                    agaagaagaCGCCCTCGGGCGTC‑TTTTTTTCTGCCTGGAAATTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGATTTCCGTTGCGGTCATTTTTTCAGGGTTTTa              <  6:312517/149‑1 (MQ=255)                                                                                                                                      aagaagaCGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTAGTTGGGCGTTTCCCAAAGATACTGCCAGCGCCAGTATTCCGTTGCGGTCATTTTTTCAGGGTTTTatg            >  5:29717/1‑147 (MQ=255)                                                                                                                                          agaCGCCCTCGTGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCTAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATATTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc         <  5:40521/148‑1 (MQ=255)                                                                                                                                             cGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGt      <  8:271589/148‑1 (MQ=255)                                                                                                                                              gCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTgg    <  7:34164/149‑1 (MQ=255)                                                                                                                                              gCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTgg    <  7:143224/149‑1 (MQ=255)                                                                                                                                              gCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTgg    >  4:231970/1‑149 (MQ=255)                                                                                                                                                ccTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCACTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGt  >  7:297759/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                    ACAACTCATCCAAAGCACCCTGCCCGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTC‑TTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGT  >  NC_002947/5390286‑5390577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑