Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,430,392	A→G	12.2%	intergenic (‑74/+87)	PP_4769 ← / ← PP_4770	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,430,392	0	A	G	12.2%	121.6 / 6.8	50	intergenic (‑74/+87)	PP_4769/PP_4770	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/24);  new base G (4/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

AAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGG  >  NC_002947/5430245‑5430540                                                                                                                                                      |                                                                                                                                                       caagggCAGCCGTGGGCCTGGCGAACGGGGTAGTCGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCcaca                                                                                                                                                        <  8:65363/144‑1 (MQ=255)     ggCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAt                                                                                                                                                   >  7:252116/1‑149 (MQ=255)      gCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGAACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGTGGGCGCT‑TTGCGC‑CA‑CTTCGCGGGCACGCCCGCTACCACGGGtgtacg                                                                                                                                                >  8:48617/1‑144 (MQ=25)              ggtccTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCTCGA‑TCTTTTTGACCTGGGAACCTCATTTTTTGCCTGGAGTGCCG‑TTGGGCTGCTGCTCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGt                                                                                                                                           <  4:248543/144‑1 (MQ=255)                gCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCcaca                                                                                                                                                        <  4:190945/133‑1 (MQ=255)                gCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCcaca                                                                                                                                                        >  3:190945/1‑133 (MQ=255)                      cgcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                 <  1:87880/149‑1 (MQ=255)                      cgaaCGGTGTAGTAGAGGTTCTGCAAATCGGCGAACAGCGCGA‑TCTTTTTCCACTGGGCACCTCATTTTTGGCCTGGAGTGCC‑‑GGTGGC‑GCTTTGCGCCCCTTCGCGGGCACGCCCGCTCCCAAGGGTGTACGCGCG‑ATACCTGTGGGa                                                                                                                               <  7:48617/146‑1 (MQ=9)                            gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCgg                                                                                                                           <  7:256116/149‑1 (MQ=255)                               tagtagAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGc                                                                                                                         <  8:39489/148‑1 (MQ=255)                                 gtagAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAg                                                                                                                              >  3:105916/1‑141 (MQ=37)                                 gtagAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAg                                                                                                                              <  4:105916/141‑1 (MQ=37)                                 gtagAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTg                                                                                                                      >  3:266625/1‑149 (MQ=255)                                  tagAGGTTCTGCACATCGGCGAACAGCGCGATTCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTg                                                                                                                      >  1:46479/1‑149 (MQ=255)                                  tagAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGc                                                                                                                     <  1:139047/149‑1 (MQ=255)                                            gCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAggg                                                                                                          >  5:117929/1‑149 (MQ=255)                                             cacaTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAgggg                                                                                                         <  3:351767/149‑1 (MQ=255)                                             cacaTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAg                                                                                                            <  5:388607/146‑1 (MQ=255)                                             cacaTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCGGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAggg                                                                                                          <  4:222475/148‑1 (MQ=255)                                              acaTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGgc                                                                                                        <  3:156162/149‑1 (MQ=255)                                               caTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAg                                                                                                            >  1:110533/1‑144 (MQ=255)                                               caTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAg                                                                                                            <  2:110533/144‑1 (MQ=255)                                               caTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCCTCGCGGGCACCCCCCCGCCCCCCGGGAT‑AGCGGGGGACCCCGGGGGCGGGGGGGTGCCC‑GCGCAgggggg                                                                                                       >  6:207669/1‑147 (MQ=255)                                                   ggCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCaaa                                                                                                   <  2:88287/149‑1 (MQ=255)                                                    gCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAg                                                                                                  >  7:54752/1‑149 (MQ=255)                                                                 attttttcCCCCGGGGACCCCCATTTTGCGCCTGGATGGCCG‑GGGCGCGGCGCCCCCGACCTCCGCGGACACCCCCGCTCCACCCCGTCT‑GGCGGGTACCCCGGTGGGTGGGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGaaa                                                                                      <  4:198764/146‑1 (MQ=255)                                                                      tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGTTAGTGGGCGTGCCCTGCGAAGGGGCGCAAAGAGCCCCCTGAAATGc                                                                                   <  1:41680/148‑1 (MQ=255)                                                                      tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGc                                                                                   >  3:207303/1‑147 (MQ=255)                                                                      tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCCTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAAt                                                                                     <  5:207669/145‑1 (MQ=255)                                                                       ttCTCCTGGGAAGCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCca                                                                                 <  4:356660/148‑1 (MQ=255)                                                                            cTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTa                                                                           >  1:96488/1‑149 (MQ=255)                                                                               ggAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCTCAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCG‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTAt                                                                          <  3:34054/147‑1 (MQ=255)                                                                                 aaCCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCa                                                                      <  4:266625/149‑1 (MQ=255)                                                                                    cTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGCAGGGGCGCAACGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc                                                                   >  8:154969/1‑149 (MQ=255)                                                                                    cTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc                                                                   <  5:113980/149‑1 (MQ=255)                                                                                    cTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACACCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc                                                                   >  3:135008/1‑149 (MQ=255)                                                                                    cTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCGGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc                                                                   >  6:370457/1‑149 (MQ=255)                                                                                     tCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAcc                                                                  >  5:163332/1‑149 (MQ=255)                                                                                     tCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCCCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc                                                                   >  1:115476/1‑148 (MQ=255)                                                                                        tttttGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGt                                                               <  2:238120/149‑1 (MQ=255)                                                                                            tgtccTGGAGTGCCG‑GGGGGCGGCTGCGCAGACCTTCGCGGACACGGCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAg                                                            <  6:230030/145‑1 (MQ=255)                                                                                             ggCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGa                                                           <  4:207303/148‑1 (MQ=255)                                                                                               ccTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACg                                                         >  5:367045/1‑148 (MQ=255)                                                                                                         cG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc                                              <  8:252116/149‑1 (MQ=255)                                                                                                         cG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc                                              <  3:79088/149‑1 (MQ=255)                                                                                                                              cTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCga                                        >  3:146850/1‑135 (MQ=255)                                                                                                                              cTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCga                                        <  4:146850/135‑1 (MQ=255)                                                                                                                                   cgGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGGATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCg                     >  5:190469/1‑149 (MQ=255)                                                                                                                                      acacGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCTGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTg                  >  8:103695/1‑149 (MQ=255)                                                                                                                                       cacGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGc                 <  8:54752/149‑1 (MQ=255)                                                                                                                                        acGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGc                 >  3:370577/1‑148 (MQ=37)                                                                                                                                            ccGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGCCC‑GCGACGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaaa            >  7:248244/1‑149 (MQ=37)                                                                                                                                                  ccACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCg      >  5:73562/1‑149 (MQ=255)                                                                                                                                                    acaGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTc    >  7:110340/1‑149 (MQ=255)                                                                                                                                                      aGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCgg  >  2:161321/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                       AAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCG‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGG  >  NC_002947/5430245‑5430540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑