Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,485,154	T→C	18.7%	intergenic (+60/‑87)	purH → / → purD	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,485,154	0	T	C	18.7%	57.5 / 9.4	33	intergenic (+60/‑87)	purH/purD	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base T (8/18);  new base C (3/3);  total (11/22)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

GTTCGATGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCC  >  NC_002947/5485006‑5485300                                                                                                                                                     |                                                                                                                                                   gtTCGATGCGTGATGCTGAAGTGATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAt                                                                                                                                                    <  8:156221/149‑1 (MQ=255)    cGAGGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGGCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAtggt                                                                                                                                                 <  7:74678/149‑1 (MQ=255)     gATGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAtggtg                                                                                                                                                <  1:242570/149‑1 (MQ=255)      aTGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCGTTCCCGCGAATACGCtggtgg                                                                                                                                               >  5:312403/1‑149 (MQ=255)            gATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAtggt                                                                                                                                                 <  2:197429/141‑1 (MQ=255)            gATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGAtggt                                                                                                                                                 >  1:197429/1‑141 (MQ=255)              tGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCTGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATTGTGGATTCAccg                                                                                                                                       <  4:73446/149‑1 (MQ=255)                   aaGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCAccatcgta                                                                                                                                  >  6:332725/1‑143 (MQ=37)                    aGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCAt                                                                                                                                 <  4:192320/149‑1 (MQ=255)                      tCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTc                                                                                                                               <  5:332725/149‑1 (MQ=37)                           gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTCGCgg                                                                                                                           <  4:164226/148‑1 (MQ=37)                           gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg                                                                                                                           <  1:48950/148‑1 (MQ=255)                           gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg                                                                                                                           <  7:143861/148‑1 (MQ=255)                           gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg                                                                                                                           <  5:10887/148‑1 (MQ=255)                                  ccGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGcc                                                                                                                    <  8:124451/148‑1 (MQ=255)                                     acgaGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCt                                                                                                                <  6:368476/149‑1 (MQ=255)                                        aGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCTTGCCCGcgaat                                                                                                             <  2:245082/149‑5 (MQ=255)                                                 tCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTcgcg                                                                                                                            <  2:42685/125‑1 (MQ=21)                                                 tCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTcgcg                                                                                                                            >  1:42685/1‑125 (MQ=21)                                                          tCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGGATACGATGGTGGGTTAACCGCCGCCTTAGCGGGGCAGGCCGCGGCCCCAGTGTTAGGGGGAAGc                                                                                           >  3:100897/1‑149 (MQ=255)                                                              cACTGGCAGGCGCCACTCCCCCCACTAATTACGGGGATCCCGCGGCAAGTCGAGAGCCTGTGGGAGCGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCAc                                                                                        <  7:221370/148‑1 (MQ=38)                                                               acggccctGCCCCACTTCCCCCAATAATTACGCGTAGCCCGAGGAATGGCGATATCCTGTGGGCGCGGGCTTGCCCGCTAATACTATGGTGTATTCACCGCCGCATTCGCGGGAAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACgg                                                                                      <  4:100897/142‑1 (MQ=255)                                                               aCTGGCAGGCCCCACTTCCGCCACTAATACCGCGGATCCCGCGGCAAGGCCAGACCCTGTGGGCGCGGGCTTGCCGGCTAATACTTTGGTGGATTCCCCGCCGCATTCGCGGGAAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACgg                                                                                      <  6:312403/149‑1 (MQ=255)                                                                        cgcCACTTCCGCCACAAATTCCCCGCATCCCGCGCCAAGGCGAGATCCTGTGGGCGCGGGCTTGCCCGCGAATACGAGGGTGGATCCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACtt                                                                              <  5:342123/148‑1 (MQ=37)                                                                                   ccACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg                                                                                                                           <  6:205381/92‑1 (MQ=255)                                                                                   ccACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg                                                                                                                           >  5:205381/1‑92 (MQ=255)                                                                                       tAATTACGCGGATCCCGAGCCACGTCGATAACCTGTGGGAGCGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGAc                                                              <  5:280788/149‑1 (MQ=38)                                                                                                          gCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGTATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGatcatc                                            <  6:179591/148‑1 (MQ=255)                                                                                                                 gagaTCCTGTGGGAGCGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGtttt                                                                 <  1:74354/120‑1 (MQ=255)                                                                                                                                     aagCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGa                                                               >  7:256836/3‑102 (MQ=255)                                                                                                                                     aagCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGa                                                               <  8:256836/100‑1 (MQ=255)                                                                                                                                       gCCCGCGAATGCGGCGGTGAATCCACCATCGTATTCGCGGGCAAGCCCGCTCAGACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGCTAATCGGCAGCGGTGGCCGTGAGCACGCcctgtcc              >  6:316454/1‑149 (MQ=25)                                                                                                                                         cggctaaTCCGATGGTGGATTCACCGCCGAATTCGCGGCAAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTATGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg            <  7:108449/144‑1 (MQ=255)                                                                                                                                         ccGCGAATACGATGGTGTATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg            >  3:245203/1‑149 (MQ=255)                                                                                                                                         ccGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg            >  5:326340/1‑149 (MQ=255)                                                                                                                                           gcgAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctgg           >  7:52281/1‑148 (MQ=255)                                                                                                                                              aaTACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAg       >  7:135896/1‑149 (MQ=255)                                                                                                                                                   tttggtgtATTCACCGCGGCTTTGGCGGGCATGCGCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGcc  <  4:373397/147‑1 (MQ=255)                                                                                                                                                   gATGGTGTATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGcc  <  4:245203/149‑1 (MQ=255)                                                                                                                                                   gATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGcc  >  7:180914/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GTTCGATGCGTGATGCTGAAGTCATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATCCTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCC  >  NC_002947/5485006‑5485300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑