Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 68,591 T→C 6.2% I228V (ATT→GTT)  PP_0058 ← 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294768,5910TC 6.2% 237.3 / 4.3 80I228V (ATT→GTT) PP_00581‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (37/38);  new base C (3/2);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

CAAGCATTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGC  >  NC_002947/68443‑68735
                                                                                                                                                    |                                                                                                                                                
cAAGCATTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAAt                                                                                                                                                  >  5:181673/1‑149 (MQ=255)
 aaGCATTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGTAATg                                                                                                                                                 <  3:127688/149‑1 (MQ=255)
 aaGCATTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAAt                                                                                                                                                  <  5:293967/148‑1 (MQ=255)
 aaGCATTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATg                                                                                                                                                 >  2:166895/1‑149 (MQ=255)
   ccATCTAATCCAAAAACCTCACACACGTTCTCCACAGAACGGCAAGCCAACTCCTCCTGAGGTTCTTCCGCAGCTGGCGGCAGCGATCCCAAGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGc                                                                                                                                               <  8:326826/148‑1 (MQ=255)
     aTTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCAc                                                                                                                                             <  6:287995/149‑1 (MQ=255)
             cAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGcccc                                                                                                                                     <  3:163562/149‑1 (MQ=255)
              aaaaaCTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCg                                                                                                                                    >  8:187299/1‑149 (MQ=255)
              aaaaaCTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCg                                                                                                                                    >  6:363632/1‑149 (MQ=255)
                  aCTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGcc                                                                                                                                <  3:71267/149‑1 (MQ=255)
                         acaAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCg                                                                                                                         >  6:361409/1‑149 (MQ=255)
                            aGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTAc                                                                                                                      <  5:361409/149‑1 (MQ=255)
                                  ccACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCGATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGGAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTCCAGATGGCGTACATCGGc                                                                                                                >  8:86395/1‑149 (MQ=255)
                                   cacaGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCt                                                                                                               >  4:97748/1‑149 (MQ=255)
                                    acaGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCt                                                                                                               >  7:251117/1‑148 (MQ=255)
                                      aGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCAc                                                                                                            >  7:61195/1‑149 (MQ=255)
                                      aGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCAc                                                                                                            >  6:56933/1‑149 (MQ=255)
                                         aGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCaa                                                                                                         >  4:151903/1‑149 (MQ=255)
                                           gTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTGACCAAt                                                                                                        >  1:209060/1‑148 (MQ=255)
                                           gTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAAt                                                                                                        >  1:207599/1‑148 (MQ=255)
                                           gTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAAt                                                                                                        >  1:217530/1‑148 (MQ=255)
                                             cAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTAc                                                                                                     >  2:333323/1‑149 (MQ=255)
                                                   aTCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGTCGTTGGCGTACATCGGCTCACCAATTACCACTTc                                                                                               <  2:86811/149‑1 (MQ=255)
                                                   aTCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCCATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTc                                                                                               <  5:363632/149‑1 (MQ=255)
                                                     cTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGAAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGACCCGTGCCGGTGGCGGACAACGGCTAACCAATTAACACTTCGa                                                                                             >  8:283791/1‑149 (MQ=255)
                                                        cTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCg                                                                                              <  2:207599/145‑1 (MQ=255)
                                                        cTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGa                                                                                             <  7:187299/146‑1 (MQ=255)
                                                        cTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGAt                                                                                            <  3:380783/147‑1 (MQ=255)
                                                          ccTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGa                                                                                             >  2:372688/1‑144 (MQ=255)
                                                          ccTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGa                                                                                             <  1:372688/144‑1 (MQ=255)
                                                           cTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTAc                                                                                       >  8:283455/1‑149 (MQ=255)
                                                              aGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCg                                                                                     <  5:242723/148‑1 (MQ=255)
                                                               ggTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCgg                                                                                    >  8:92083/1‑148 (MQ=255)
                                                                 tttttCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCAc                                                                                 >  5:65050/1‑149 (MQ=255)
                                                                 tttttCAGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAAtt                                                                                                       >  6:37933/1‑127 (MQ=255)
                                                                 tttttCAGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAAtt                                                                                                       <  5:37933/127‑1 (MQ=255)
                                                                  ttttCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACg                                                                                >  3:66197/1‑149 (MQ=255)
                                                                   tttCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACg                                                                                <  2:217530/148‑1 (MQ=255)
                                                                    ttCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGc                                                                               <  3:207405/148‑1 (MQ=255)
                                                                      ccGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTg                                                                            >  7:85689/1‑149 (MQ=255)
                                                                        gCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGcc                                                                          <  3:97748/149‑1 (MQ=255)
                                                                         cAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGccc                                                                         <  7:283455/149‑1 (MQ=255)
                                                                         cAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGccc                                                                         <  6:328340/149‑1 (MQ=255)
                                                                         cAGCTGGCGGCAGCGATCCCATCGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGAACGCTTGccc                                                                         >  6:203091/1‑149 (MQ=255)
                                                                               gcggcAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCg                                                                   <  5:21549/149‑1 (MQ=255)
                                                                                 ggcAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGc                                                                           <  6:96726/139‑1 (MQ=255)
                                                                                 ggcAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGc                                                                           >  5:96726/1‑139 (MQ=255)
                                                                                   cAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCGCCAACCGGt                                                                 >  7:245576/1‑147 (MQ=255)
                                                                                   cAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGt                                                                 <  8:245576/147‑1 (MQ=255)
                                                                                   cAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCt                                                               <  6:65050/149‑1 (MQ=255)
                                                                                     gCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTg                                                             <  3:106319/149‑1 (MQ=255)
                                                                                      cGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTgg                                                            <  3:371606/149‑1 (MQ=255)
                                                                                        aTCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGc                                                          >  3:240815/1‑149 (MQ=255)
                                                                                        aTCCCATCGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGc                                                          <  5:203091/149‑1 (MQ=255)
                                                                                           ccATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCCACCGGTCTTGGGc                                                          >  1:371366/1‑146 (MQ=255)
                                                                                             aTGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGaa                                                     <  7:92083/149‑1 (MQ=255)
                                                                                                            gCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgtg                                      <  4:240815/149‑1 (MQ=255)
                                                                                                            gCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgt                                       <  6:181673/148‑1 (MQ=255)
                                                                                                              ttCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTggg                                    >  7:277485/1‑149 (MQ=255)
                                                                                                                     cAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCg                              <  1:333323/148‑1 (MQ=255)
                                                                                                                     cAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGAGCAATc                               <  6:57388/147‑1 (MQ=255)
                                                                                                                     cAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGAGCAATc                               >  5:57388/1‑147 (MQ=255)
                                                                                                                      aaGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCgg                             >  5:390163/1‑148 (MQ=255)
                                                                                                                       aGCTGCAGCGCGGTCGTTGAGTTCTGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGTCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGgca                           <  5:123744/149‑1 (MQ=255)
                                                                                                                            cAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCAcc                      >  8:250417/1‑149 (MQ=255)
                                                                                                                             aGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCAAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCg                     >  7:42555/1‑149 (MQ=255)
                                                                                                                              gcgcGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCgg                    <  8:277485/149‑1 (MQ=255)
                                                                                                                                gcgGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTATCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCa                  <  5:56933/149‑1 (MQ=255)
                                                                                                                                  ggTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTggg                                    >  7:182266/1‑129 (MQ=255)
                                                                                                                                  ggTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTggg                                    <  8:182266/129‑1 (MQ=255)
                                                                                                                                    tCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGccc              >  7:191853/1‑149 (MQ=255)
                                                                                                                                      gTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCg             <  8:251117/148‑1 (MQ=255)
                                                                                                                                            ttCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTc       >  2:224481/1‑148 (MQ=255)
                                                                                                                                            ttCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCa      >  4:379164/1‑149 (MQ=255)
                                                                                                                                              cGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCAc     <  8:85689/148‑1 (MQ=255)
                                                                                                                                                gCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCgg                    <  7:335213/131‑1 (MQ=255)
                                                                                                                                                gCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGc  >  3:248694/1‑149 (MQ=255)
                                                                                                                                                ccAACGGCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgtg                                      <  7:45223/112‑1 (MQ=255)
                                                                                                                                                ccAACGGCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgtg                                      >  8:45223/2‑113 (MQ=255)
                                                                                                                                                ccAACGGCACGGGGCCACGTGCCATTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGc  >  7:193206/2‑149 (MQ=255)
                                                                                                                                                 cAACGGCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCAc     <  2:10593/145‑1 (MQ=255)
                                                                                                                                                 cAACGGCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCAc     >  1:10593/1‑145 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAAGCATTTAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGC  >  NC_002947/68443‑68735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑