Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,627,338	T→A	8.9%	E55V (GAA→GTA) ‡	PP_4944 ←	carbamoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,627,338	0	T	A	8.9%	194.5 / 7.2	67	E55V (GAA→GTA) ‡	PP_4944	carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base A (4/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

ACGATTGCCCATTAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCGGACAGGCC  >  NC_002947/5627190‑5627483                                                                                                                                                     |                                                                                                                                                  acgaTTGCCCATTAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGtt                                                                                                                                                   >  2:110623/1‑149 (MQ=255)  cgaTTGCCCATTAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTc                                                                                                                                                  <  7:280835/149‑1 (MQ=255)    aTTGCCCATTAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCa                                                                                                                                                <  8:185538/149‑1 (MQ=255)     ttGCCCATTAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAg                                                                                                                                               <  2:106359/149‑1 (MQ=255)        cccATTAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGc                                                                                                                                             <  2:350547/148‑1 (MQ=255)          cATTAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAg                                                                                                                                           >  6:98183/1‑148 (MQ=255)          cATTAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGGAACAGGCAgg                                                                                                                                          <  5:202327/149‑2 (MQ=255)           aTTAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCTGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGGAACAGGCAggc                                                                                                                                         <  3:245233/149‑3 (MQ=255)            ttAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGtt                                                                                                                                                   <  8:311095/138‑1 (MQ=255)            ttAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGtt                                                                                                                                                   >  7:311095/1‑138 (MQ=255)                  tGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGcc                                                                                                                                  >  8:115522/1‑149 (MQ=255)                  tGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGcc                                                                                                                                  >  4:250771/1‑149 (MQ=255)                     cATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCgcc                                                                                                                               >  6:257510/1‑147 (MQ=255)                      aTCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACt                                                                                                                              <  4:196032/149‑1 (MQ=255)                        cGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCg                                                                                                                            >  7:149731/1‑149 (MQ=255)                           gTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCa                                                                                                                                                >  4:334886/1‑126 (MQ=255)                           gTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCa                                                                                                                                                <  3:334886/126‑1 (MQ=255)                            tGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAgg                                                                                                                        <  7:115522/149‑1 (MQ=255)                              cACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAggg                                                                                                                       >  4:147530/1‑148 (MQ=255)                               aCGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCa                                                                                                                     >  6:274373/1‑149 (MQ=255)                               aCGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCa                                                                                                                     >  7:70815/1‑149 (MQ=255)                                 gATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATg                                                                                                                   <  3:250771/149‑1 (MQ=255)                                 gATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATg                                                                                                                   >  5:202929/1‑149 (MQ=255)                                          cGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGttcttc                                                                                                          <  7:23595/149‑1 (MQ=255)                                            tACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCTTAGGGCATGCGGTTCTTCGc                                                                                                        <  2:114836/149‑1 (MQ=255)                                                 aTAGCGCTTGGAATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTAACACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCt                                                                                                   >  8:164225/1‑149 (MQ=255)                                                  tAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCtt                                                                                                  <  5:98183/149‑1 (MQ=255)                                                    gcgcTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGt                                                                                                >  4:35726/1‑149 (MQ=255)                                                       cTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTcac                                                                                             <  6:364005/149‑1 (MQ=255)                                                       cTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTcac                                                                                             >  5:134286/1‑149 (MQ=255)                                                                gTGCCACCGCGCCTTGCCGAACAGGCTGATTGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAgc                                                                                    >  6:198752/1‑149 (MQ=255)                                                                gTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAg                                                                                     <  5:57405/148‑1 (MQ=255)                                                                      ccGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCtctt                                                                              <  5:153493/149‑1 (MQ=255)                                                                            cTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGc                                                                                                                                   <  2:219305/90‑1 (MQ=255)                                                                            cTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGc                                                                                                                                   >  1:219305/1‑90 (MQ=255)                                                                             ttGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCGCGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTcggc                                                                       <  5:318947/149‑1 (MQ=255)                                                                                 cGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGATTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTcggcggcg                                                                   <  1:110623/149‑1 (MQ=255)                                                                                  gAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTcggcggcgg                                                                  >  7:214129/1‑149 (MQ=255)                                                                                  gAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTcggcggcgg                                                                  >  2:234221/1‑149 (MQ=255)                                                                                        gCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGttct                                                                                                            >  6:348394/1‑101 (MQ=255)                                                                                        gCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCa                                                            <  3:147530/149‑1 (MQ=255)                                                                                              cGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTg                                                       <  8:70815/148‑1 (MQ=255)                                                                                                  gCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGcc                                                     >  2:168035/1‑146 (MQ=255)                                                                                                  gCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGcc                                                     <  1:168035/146‑1 (MQ=255)                                                                                                   cGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTc                                                  >  8:322318/1‑148 (MQ=255)                                                                                                   cGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTc                                                  >  4:248028/1‑148 (MQ=255)                                                                                                                  ccACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCtctt                                                                              >  7:137711/1‑105 (MQ=255)                                                                                                                  ccACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCcgcg                                  >  1:333557/1‑149 (MQ=255)                                                                                                                  ccACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCCCATGCTTGTCACGCACGAAGCGCtctt                                                                              <  8:137711/105‑1 (MQ=255)                                                                                                                   cACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCgg                                 <  5:198752/149‑1 (MQ=255)                                                                                                                       tcgacgtcgGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAgg                             <  8:214129/149‑1 (MQ=255)                                                                                                                       tcgacgtcgGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAgg                             <  7:322318/149‑1 (MQ=255)                                                                                                                       tcgacgtcgGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGGAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAgg                             <  6:202929/149‑1 (MQ=255)                                                                                                                            gtcgGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGTGTCAt                        <  4:369259/149‑1 (MQ=255)                                                                                                                            gtcgGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCAt                        <  7:164225/149‑1 (MQ=255)                                                                                                                                 aCGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCt                    >  1:2019/1‑148 (MQ=255)                                                                                                                                  cGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTaa                  <  3:35726/149‑1 (MQ=255)                                                                                                                                   ggCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCATGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAg                 >  1:14283/1‑149 (MQ=255)                                                                                                                                   ggCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAg                 <  6:134286/149‑1 (MQ=255)                                                                                                                                   ggCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAg                 >  4:213975/1‑149 (MQ=255)                                                                                                                                    gCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAgg                <  2:2019/149‑1 (MQ=255)                                                                                                                                      ttGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAACGTACAGGGCCGCGGAAGGGTCATGGCTAAGGgc              >  1:348944/1‑149 (MQ=255)                                                                                                                                          gttctgcctgGAACAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCg          >  3:235349/4‑149 (MQ=255)                                                                                                                                          gttctgcctgGAACAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGACg          >  5:363035/4‑149 (MQ=255)                                                                                                                                           ttctgcctgGAACAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCg          >  2:317518/3‑148 (MQ=255)                                                                                                                                           ttctgcctgGAACAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCg          >  3:76530/3‑148 (MQ=255)                                                                                                                                            cctgcctgTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCGGa        >  7:313596/1‑149 (MQ=255)                                                                                                                                             ctgcctgTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCGGa        >  2:146536/1‑148 (MQ=255)                                                                                                                                                 ctgTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCGGACAGGc   >  3:85353/1‑149 (MQ=255)                                                                                                                                                   gTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCGGACAGGcc  >  6:130924/1‑148 (MQ=255)                                                                                                                                                    ttCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCGGACAGGcc  >  8:308618/1‑147 (MQ=255)                                                                                                                                                    ttCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCGGACAGGcc  >  6:230597/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                                  ACGATTGCCCATTAGGATGGCATCGAGTGCACGATCCGGGGCGTACCAATAGCGCTTGGCATAGTGCCACCGCGCCTTGCCGAACAGGCTGATCGGGGCGAACGGGATGGCTACCACGTCGACGTCGGACGGCTTGATGCCTGCCTGTTCCAGGCAGAACTTCGCCGACTCGTAGGGCATGCGGTTCTTCGCATGCTTGTCACGCACGAAGCGCTCTTCTTCGGCGGCGGCAATCAGCTTGCCGTCAATGTACAGGGCCGCGGAAGGGTCATGGCTAAGGGCGCCGGACAGGCC  >  NC_002947/5627190‑5627483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑