| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,674,753 | G→C | 100% | intergenic (+115/+25) | rhlE‑II → / ← yceI | ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,674,753 | 0 | G | C | 100.0% | 197.6 / NA | 58 | intergenic (+115/+25) | rhlE‑II/yceI | ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (30/28); total (30/28) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
GCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGC‑G‑CGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC > NC_002947/5674617‑5674890 | gctgctGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCAGCAGAGAAATTCTTCATATCTAAAGGAGCGGCCATGTGTCGTGATAGG‑GCCGCAAAGC‑GTCCCCCTCGGttt > 8:91103/1‑148 (MQ=37) ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGc < 2:317328/149‑1 (MQ=255) aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTgg > 6:394116/1‑149 (MQ=255) cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTgg > 5:46457/1‑146 (MQ=255) gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAg > 6:380444/1‑148 (MQ=255) gggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAAt > 7:361596/1‑148 (MQ=255) gggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAAt > 7:195555/1‑148 (MQ=255) ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTa < 1:314100/149‑1 (MQ=255) cTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGCGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAc < 1:269875/149‑1 (MQ=37) cTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACt > 1:361798/1‑149 (MQ=255) gATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc > 1:254022/1‑149 (MQ=255) aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc < 1:38432/148‑1 (MQ=255) aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCCGTTTTCAGCTGGCAGAAATTACTTCt > 4:13314/1‑149 (MQ=255) aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt > 5:185870/1‑149 (MQ=255) gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTca < 2:254022/149‑1 (MQ=255) aGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt < 1:52815/149‑1 (MQ=255) gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc < 8:361596/149‑1 (MQ=255) ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGac < 7:64276/149‑1 (MQ=255) tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACt > 5:151614/1‑149 (MQ=255) aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcca < 3:168265/149‑1 (MQ=255) cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTTAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccagg > 2:135106/1‑149 (MQ=255) aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg < 3:13314/148‑1 (MQ=255) cacaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTg < 6:46457/149‑1 (MQ=255) acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc < 7:91103/149‑1 (MQ=255) aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg > 1:68305/1‑148 (MQ=255) gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAg > 4:321234/1‑148 (MQ=255) cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa < 6:330031/144‑1 (MQ=255) cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa > 5:330031/1‑144 (MQ=255) gCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg > 6:213464/1‑148 (MQ=255) tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg > 5:157546/1‑149 (MQ=255) cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg < 2:361798/148‑1 (MQ=255) agaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGt < 6:136288/136‑1 (MQ=255) agaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGt > 5:136288/1‑136 (MQ=255) agaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt > 3:341264/1‑149 (MQ=255) gaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat < 3:78249/111‑1 (MQ=255) gaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat > 4:78249/1‑111 (MQ=255) aaTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt < 7:73929/149‑1 (MQ=255) tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt < 5:380444/148‑1 (MQ=255) gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGc < 3:321234/146‑1 (MQ=255) gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg > 3:344129/1‑148 (MQ=255) tctcTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa < 3:345557/148‑1 (MQ=255) ctctACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGt > 2:77960/1‑149 (MQ=255) tctACAGGAGCGGCCTTGTCTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGACTGCGAAGTAGCACCCGGGCCTTTGATTCCGAAGTc > 4:160799/1‑149 (MQ=255) cAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt < 1:77960/148‑1 (MQ=255) aGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTTATATCCAGGTCCAGGGTCTGCTAAGTAGCACCCGGGCCTTTGATGCCGAATTCgttcaggt < 6:185870/149‑1 (MQ=39) gCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggtt < 5:213464/149‑1 (MQ=255) ccTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag < 3:18827/91‑1 (MQ=255) ccTTGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag > 4:18827/1‑91 (MQ=255) ttGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt > 7:213826/1‑149 (MQ=255) ttGTGTCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt > 7:223142/1‑149 (MQ=255) gtgtCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtgg > 5:327403/1‑149 (MQ=255) tCGTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg < 6:327403/148‑1 (MQ=255) gTGATAGG‑GCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg > 3:150137/1‑149 (MQ=255) gCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa < 5:394116/149‑1 (MQ=255) gCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa < 8:195555/149‑1 (MQ=255) gCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa < 2:38798/149‑1 (MQ=255) ccGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAg > 3:228900/1‑149 (MQ=255) cGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGCCACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc > 7:180125/1‑149 (MQ=255) | GCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGG‑GCCGCAAAGC‑G‑CGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC > NC_002947/5674617‑5674890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |