Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,704,540 G→C 11.3% intergenic (+65/+47) PP_5006 → / ← PP_5007 TetR family transcriptional regulator/polyhydroxyalkanoate granule‑associated protein GA2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,704,5400GC11.3% 103.5 / 5.4 43intergenic (+65/+47)PP_5006/PP_5007TetR family transcriptional regulator/polyhydroxyalkanoate granule‑associated protein GA2
Reads supporting (aligned to +/- strand):  ref base G (20/18);  new base C (3/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

TGATGATGCTGGTGGCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTGGCAG  >  NC_002947/5704393‑5704687
                                                                                                                                                    |                                                                                                                                                    
tgatgaTGCTGGTGGCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGgc                                                                                                                                                     <  5:134474/149‑1 (MQ=255)
                gccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑g                                                                                                                                    >  5:88144/1‑149 (MQ=255)
                 ccgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑gg                                                                                                                                   <  1:193867/149‑1 (MQ=255)
                  cgcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGATGCGCCAAACCTTGTAGGAGC‑ggg                                                                                                                                  <  6:361904/149‑1 (MQ=37)
                   gcATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGATGCGCCAAACCTTGTAGGAGC‑GGGt                                                                                                                                 <  5:65616/149‑1 (MQ=37)
                     aTCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑gg                                                                                                                                   >  1:165111/1‑145 (MQ=255)
                     aTCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑gg                                                                                                                                   <  2:165111/145‑1 (MQ=255)
                          caggcccaggccCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTAccc                                                                                                                           <  4:132069/148‑1 (MQ=255)
                          caggcccaggccCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCcg                                                                                                                          <  7:142000/149‑1 (MQ=255)
                             gcccaggcccGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCcgcg                                                                                                                        >  2:239010/1‑148 (MQ=255)
                             gcccaggcccGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCcgcg                                                                                                                        >  6:79956/1‑148 (MQ=255)
                              cccaggcccGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGGATCTTGTAGGAGC‑GGGTTTACCcgcg                                                                                                                        >  5:292686/1‑147 (MQ=255)
                               ccaggcccGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCcgcg                                                                                                                        >  7:340539/1‑146 (MQ=255)
                                 aggcccGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCgaagaa                                                                                                                   <  5:224991/149‑1 (MQ=255)
                                           ggcggcggAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCgg                                                                                                         >  6:773/1‑149 (MQ=255)
                                                  gacccgcTGGCAGTCACGACCCGGGAGGGGTCGGCCGGCGGGCGATGTCGTGTGGCCGCCGCCGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTGGTCGGAGG‑GGGTTTACCCGCGAAGAAGGCGACGCGGTatcaga                                                                                                  <  6:311938/144‑1 (MQ=255)
                                                          ggCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCGCCT‑ACAAGGGTTGGCGCATCGTGTAGGCGC‑GGGTTTACCCGCGACGAAGGCGCCGCGGTATCAGATCAAGGTa                                                                                          >  3:228001/1‑149 (MQ=255)
                                                                 aaGTACCTGGCGGGGTAGGCCGGCTGGAGATGTTGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGTGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGc                                                                                   <  2:15673/149‑1 (MQ=255)
                                                                     aCCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAAACCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGt                                                                               >  3:377006/1‑149 (MQ=255)
                                                                          gAGGGGTAGGCCTGCTGGTGATGTTGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCGGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTg                                                                          <  5:130893/149‑1 (MQ=255)
                                                                          gAGGGGTAGGCCTGCTGGAGTTGTCGTGTTCCCGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGt                                                                           <  1:178412/148‑1 (MQ=255)
                                                                                       gctgGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGtgct                                                             >  1:243660/1‑149 (MQ=255)
                                                                                          ggAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTgctgct                                                          >  2:92699/1‑149 (MQ=255)
                                                                                           gagaTGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGctgctg                                                         <  5:773/149‑1 (MQ=255)
                                                                                           gagaTGTAGTGTTGCAGCCGACGCATTCGCGGGTAAAACCCCTGCTAAAAAGGTTTGG‑GGGTATTGTAGGGGCGGGGTTTACCC‑CGAAGAAGGGGGCGCGGGTTGAGATAAGGGTTCCGGTGGCAGTCGGGGGCGACGGGGtggggcgg                                                         >  4:192814/1‑143 (MQ=255)
                                                                                                 tAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCgg                                                    <  3:192814/148‑1 (MQ=255)
                                                                                                 tAGGGTTCCAGCCGACCCATTCGCGGGCAACCCCCCTCCC‑ACACGGTTGGGCGCCTCTTGTAGGAGC‑GGGATTACCCGCGAAGAAGGCGACGCGGTATCAGATCCGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCggg                                                   <  8:340539/149‑1 (MQ=37)
                                                                                                    ggtTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTTCTGCTGGCCGGGGTg                                                <  2:157915/148‑1 (MQ=255)
                                                                                                          aGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGtg                                                               <  5:303910/128‑1 (MQ=255)
                                                                                                          aGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGtg                                                               >  6:303910/1‑128 (MQ=255)
                                                                                                              gACGCATTCGCGGGTAAACCCGCTGCT‑ACAAGGTTTGGGGCATGTTGTTGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGGATGAGCTGAGGGGACCGGTGGCAGTGGGTGGCGCCGGGGTGGTGCTGGGCGGGGTGGCGGGAGTTg                                      >  4:273011/1‑149 (MQ=255)
                                                                                                                       gcgGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCtg                             <  4:377006/149‑1 (MQ=255)
                                                                                                                         gggTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCtgtg                           >  1:188305/1‑149 (MQ=255)
                                                                                                                          ggTAACCCCGCTCCT‑ACACGGTTTGGCGCATCTTGTCGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTgg                          <  4:226911/149‑1 (MQ=255)
                                                                                                                                 ccGCTCCT‑ACAAGATGCGCCAAACCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGGGGGTGTTGGGGgtggtgtggtgggtgg                   >  2:156342/1‑143 (MQ=21)
                                                                                                                                   gCTCCT‑ACAAGATGCGCCAAACCTTGTAGGAGC‑GGGTTTACCCGCGAATAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAg                 >  4:66790/1‑149 (MQ=37)
                                                                                                                                   gCTCCT‑ACAAGATGCGCCAAACCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAg                 >  5:386922/1‑149 (MQ=38)
                                                                                                                                    cTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGCAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCGGTGGTCGCTGCAgg                >  5:213250/1‑149 (MQ=255)
                                                                                                                                     tCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGgc               >  5:154683/1‑149 (MQ=255)
                                                                                                                                          aCAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGATGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAg           >  6:235614/1‑149 (MQ=255)
                                                                                                                                          aCAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAg           >  6:105737/1‑149 (MQ=255)
                                                                                                                                               gTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTg      >  8:207650/1‑149 (MQ=255)
                                                                                                                                                tttGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTgg     <  1:92699/149‑1 (MQ=255)
                                                                                                                                                 ttGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTCCCGGTGCCAGGCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTGGc    <  7:207650/149‑1 (MQ=255)
                                                                                                                                                   ggCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTGGCAg  >  7:103126/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGATGATGCTGGTGGCGCCGCATCTGCAGGCCCAGGCCCGGGCGGCGGCGGAGCAGCTGGCAGTGAAGTACCTGGAGGGGTAGGCCTGCTGGAGATGTAGTGTTGCAGCCGACGCATTCGCGGGTAAACCCGCTCCT‑ACAAGGTTTGGCGCATCTTGTAGGAGC‑GGGTTTACCCGCGAAGAAGGCGACGCGGTATCAGATCAGGGTACCGGTGCCAGTCGGTGCCGACGGCGTGCTGCTGGCCGGGGTGGCGGCAGTTGCCGGTGCTGTGGTCGCTGCAGGCGCAGCGCTGGCAG  >  NC_002947/5704393‑5704687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑