Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 385,475 A→C 10.7% intergenic (+15/+20) PP_0320 → / ← ltaE methyl‑accepting chemotaxis transducer/low specificity l‑threonine aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947385,4750AC10.7% 118.1 / 2.9 43intergenic (+15/+20)PP_0320/ltaEmethyl‑accepting chemotaxis transducer/low specificity l‑threonine aldolase
Reads supporting (aligned to +/- strand):  ref base A (17/20);  new base C (3/2);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGCC  >  NC_002947/385331‑385620
                                                                                                                                                |                                                                                                                                                  
gACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAcggc                                                                                                                                                <  1:163169/149‑1 (MQ=255)
  cGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAcggcgg                                                                                                                                              <  2:226601/149‑1 (MQ=255)
       cAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCCGTGCGGcccct                                                                                                                                         >  6:159577/1‑148 (MQ=255)
         ggggATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGc                                                                                                                                       >  5:115592/1‑149 (MQ=255)
          gggATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGct                                                                                                                                      >  3:220621/1‑149 (MQ=255)
             aTTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCtctc                                                                                                                                   <  5:89899/149‑1 (MQ=255)
                  cGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCa                                                                                                                              <  6:115592/149‑1 (MQ=255)
                    gccgctcgcgTGCTACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCa                                                                                                                              <  4:38532/141‑1 (MQ=255)
                              tGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGAt                                                                                                                  <  8:226921/149‑1 (MQ=255)
                               gagaCGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATc                                                                                                                 <  5:195964/149‑1 (MQ=255)
                                     gcggcggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCgg                                                                                                           <  1:11995/149‑1 (MQ=255)
                                      cggcggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACggcggc                                                                                                                                             >  6:335484/1‑114 (MQ=255)
                                      cggcggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACggcggc                                                                                                                                             <  5:335484/114‑1 (MQ=255)
                                              gTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATccgcc                                                                                                  >  3:89229/1‑149 (MQ=255)
                                                           gcgAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCa                                                                                     >  1:62999/1‑149 (MQ=255)
                                                                  gTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCgcgc                                                                             >  5:215627/1‑149 (MQ=255)
                                                                   tGCCAGGCGTTGCGTGGGGTGGCTGATGACCTGGCGCGGCAGATGGGTGGGGTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGCATATCCGCCGCCAGCTCACGCA‑CCCGCGct                                                                            <  1:36058/149‑1 (MQ=255)
                                                                     ccAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCtctc                                                                                                                                   >  6:308086/1‑93 (MQ=255)
                                                                     ccAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCtctc                                                                                                                                   <  5:308086/93‑1 (MQ=255)
                                                                         tctttGCGGGGGTGGGCTGATGCCCGGGCGCGGCAGAGGGGTGGGTTCCGGCTCTAGAGCGCCGGGCCCGCCCGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCttcttc                                                                       <  4:30189/145‑1 (MQ=255)
                                                                            ttGGGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTa                                                                   <  1:258297/149‑1 (MQ=255)
                                                                             tGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCttcttc                                                                       >  3:30189/1‑144 (MQ=255)
                                                                              gCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATc                                                                 <  6:140466/149‑1 (MQ=255)
                                                                               cGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCCCGCA‑CCCGCGCTTCTTCGGTATcc                                                                >  7:344622/1‑149 (MQ=255)
                                                                               cGTGGGTTGGCGGATGACCTGGGGCGGGAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCTCGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCT‑CCCGCGCTTCTTCGGTATcc                                                                <  8:344622/149‑1 (MQ=255)
                                                                                    gTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGa                                                           <  1:2772/149‑1 (MQ=255)
                                                                                        gCTGATGACCTGGCGCGGCTGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCCGCGCTTCTTCGGTATCCCACGAGca                                                        <  1:4042/149‑1 (MQ=255)
                                                                                          tgatgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACAt                                                     >  6:370195/1‑149 (MQ=255)
                                                                                                  tGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgcgcg                                             >  5:159024/1‑149 (MQ=255)
                                                                                                                     gtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATa                            >  2:361839/1‑147 (MQ=255)
                                                                                                                      ttCAGGCTCTTGATCGCTGGGGCCGCAGGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAg                         <  6:215627/149‑1 (MQ=255)
                                                                                                                      ttCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAg                         <  4:89229/149‑1 (MQ=255)
                                                                                                                         aGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGta                      >  3:170361/1‑149 (MQ=255)
                                                                                                                           gCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGtata                    <  4:220621/149‑1 (MQ=255)
                                                                                                                           ctatctgATCGCCGGGCCCGCCCGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGtat                     <  6:16401/142‑1 (MQ=255)
                                                                                                                                  aTCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGtat                     >  5:16401/1‑141 (MQ=255)
                                                                                                                                    cGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAgcgc             >  1:382640/1‑147 (MQ=255)
                                                                                                                                       cggggCCGCCGTGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGcc        >  1:385372/2‑149 (MQ=255)
                                                                                                                                         gggCCGCCGTGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCCCGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGGATAGAAGCGCCAGCCCt      >  3:250643/1‑149 (MQ=255)
                                                                                                                                         gggCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCt      <  8:27156/149‑1 (MQ=255)
                                                                                                                                             cGCACGGCGGTCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGcc  >  4:7037/1‑149 (MQ=255)
                                                                                                                                             cGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCATGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTg    <  1:110840/147‑1 (MQ=255)
                                                                                                                                             cGCACGGCGGACCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTg    >  2:110840/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCA‑CCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGCC  >  NC_002947/385331‑385620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑