| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,740,457 | G→A | 13.8% | intergenic (+22/+49) | hutF → / ← PP_5037 | formiminoglutamate deiminase/lipocalin family lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,740,457 | 0 | G | A | 13.8% | 121.1 / 10.3 | 54 | intergenic (+22/+49) | hutF/PP_5037 | formiminoglutamate deiminase/lipocalin family lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (16/29); new base A (2/5); total (18/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.60e-01 | |||||||||||
CGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTC > NC_002947/5740309‑5740604 | cGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAg > 6:182891/1‑149 (MQ=255)cGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAg < 6:216291/149‑1 (MQ=255) gTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGcc < 1:204924/149‑1 (MQ=255) cGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGCACCGGGGTTGCGTTGCAACCCTTc < 3:167063/149‑1 (MQ=255) gATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTcg > 6:157215/1‑149 (MQ=255) gCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTg < 8:231778/149‑1 (MQ=255) ccAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTg > 2:288164/1‑148 (MQ=255) ccAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGc < 2:194304/149‑1 (MQ=255) gcgcGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGccc > 7:319965/1‑149 (MQ=255) gcgcGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGccc > 3:103212/1‑149 (MQ=255) cgcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCcg < 4:88364/149‑1 (MQ=255) gcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCcgc < 5:157215/149‑1 (MQ=255) gcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCcgc < 1:288164/149‑1 (MQ=255) cgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGTAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCcgcg < 3:157750/149‑1 (MQ=255) gATGTGATTGTGAATTGGCTATGTTTGGTGCGCCAGGGGCGTCATGCCCAGGAAGATGATAGCGGGCGGGCTTTTGCCAAGGTTTTGCTCCAGCTTTTGGGCTGAGCGTACCGGTGTTGCGTTGCAACCCtt < 8:192046/132‑1 (MQ=255) gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAATCGGGCGGGCGTTTGACAAGGTGTTTCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCAtt > 7:192046/1‑132 (MQ=255) aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GGGCTTGCAcgcg < 2:106248/147‑1 (MQ=255) aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GGGCTTGCAcgcg > 1:106248/1‑147 (MQ=255) tgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAg < 5:195830/149‑1 (MQ=255) tgtgATGGTGAATGGGCAAGGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCGGAGCGTACCGGGGTTGCGTTGCAACCCTTCGC‑GGGCTTGCACGCG‑AAg < 5:313839/149‑1 (MQ=38) gtgATGGTCTAGGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTGGCAGCCCTTCGC‑GTGCACGCCCGCG‑AAg < 6:2911/148‑1 (MQ=255) aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGtt < 6:368265/149‑1 (MQ=255) aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGtt < 4:103212/149‑1 (MQ=255) aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGtt < 8:319965/149‑1 (MQ=255) aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGC‑GGGCTTGCACGCG‑AAgggct < 5:83422/149‑3 (MQ=38) gAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTTGGTTTGCGCCCATGGC‑CGGGAAGCCCCCG‑GAGGGtttggaa > 5:21647/1‑144 (MQ=255) gAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GGGcttgcac < 8:117247/134‑7 (MQ=255) gAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GGGcttgcac > 7:117247/1‑128 (MQ=255) gAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTAGC‑GTGCAAGCCCGCG‑AAGGGTTgcaac > 5:284172/1‑149 (MQ=255) aaTGGGCAATGGGGGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTgcaac < 7:132993/148‑1 (MQ=255) ggcacggggTGGGGCGCCAGGGGCGGCAGGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGAACCGGGGTGGCGTGGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTgcaacgcaac < 7:66521/143‑1 (MQ=255) gccAATGGGTGGGGCCCCCGGGGCGGCATGCCCAGGAAGATGAACGCGGGCGGGGGTTTCCCACGGTGTTGCGCCAGCTTTTGGGCTCAGCGTACCGGGGTTGCGTTGCAGCCCTGCGC‑GTGCACGCCCGCT‑ATGGGTTgcaacgcaac < 6:21647/147‑1 (MQ=255) tggtgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑ATGGGTTGCACCGCCCCCCCaaaaaa > 4:79950/1‑149 (MQ=255) gcgcCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTGAGCCCATAGCGGGGAAACCCCCCA‑AAGGGTTGAAACCCACCCCAaaaaaaac > 4:320268/1‑146 (MQ=255) aGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTTCGGTTGAGCCCATAGC‑GGGGCAGACCGCGCAAGGGTTGAAACCCAACCCAaaaaaaaaagagg > 4:23656/1‑141 (MQ=255) ggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCTCCAGCTTTTGGGCTTAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑ATGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTa < 1:78668/149‑1 (MQ=255) cATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACt < 5:182891/149‑1 (MQ=255) aTGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGAACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTa < 5:118603/149‑1 (MQ=255) cAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCGGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTCCAAGCCCGCG‑AAGGTTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGc < 2:23252/149‑1 (MQ=255) attgcgggcgtGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGGGTTCCGGGGTTGCGTTGCTGCCCTTCGC‑GTGCATGCCCGCG‑AAGGGTTGCAACGGAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAc < 5:362182/145‑1 (MQ=255) aaaGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGG‑GTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAc < 7:7727/148‑1 (MQ=255) aaGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgc < 6:284172/149‑1 (MQ=255) gcgggcgTTTGCCAAGGTGTTGCCCCAGCTTTTGGGCTGAGCGTACCGGGGTGGCGTTCCAGCCCTTCGC‑GTGCAAGCCCGCG‑ATGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCa < 3:23656/149‑1 (MQ=255) tttGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTg < 7:256906/124‑1 (MQ=255) gCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCATAGC‑GTGCAAGCCCGCG‑AAGGGGTTCAACGCAACCCCAAAAAAATAAGTGCATTTTTAATATTTTCTGATAGGACGCCCGCCAGATAAAGCgg > 1:87298/1‑149 (MQ=255) cAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTcgc < 6:233645/58‑1 (MQ=255) cAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTAACGGGGTTGCGTTGCAGCCCTTcgc > 5:233645/1‑58 (MQ=255) ggTGTGCCGCCAGCTTTTGGGCGGAGCGTACGGGGGGTGCGTTGCAGCCCTTCGC‑GTGCAACCCCGCG‑AAGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgt < 1:155938/149‑1 (MQ=255) aGCGTACCGGGGTTCCGTTGCACCCCTTCGC‑GTCCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt < 1:146787/149‑1 (MQ=255) cgttccgggggTGGCTTTCCACCCGTTCGG‑GGGCACGCCCGGT‑ATGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg < 7:313555/142‑1 (MQ=255) cttACCGGGGTTGCGTTGCAGCCGTTGGC‑GTGCAAGCGCGCG‑ATGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg < 4:146643/146‑1 (MQ=255) ttgcgttgcAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATGAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGtt > 5:375629/1‑149 (MQ=255) ttgcAACCCTTCGC‑GGGCTTGCACGCG‑AAGGGCTGCAACGCTACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc < 1:325328/149‑1 (MQ=38) ttgcAACCCTTCGC‑GGGCTTGCACGCG‑AAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc > 2:325328/1‑149 (MQ=38) cAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCt > 2:2311/1‑149 (MQ=255) aGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc > 7:134804/1‑149 (MQ=255) | CGGTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGC‑GTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTC > NC_002947/5740309‑5740604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |