| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,751,746 | A→C | 9.0% | intergenic (‑184/‑287) | thiI ← / → glnA | tRNA sulfurtransferase/glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,751,746 | 0 | A | C | 9.0% | 150.9 / 7.4 | 56 | intergenic (‑184/‑287) | thiI/glnA | tRNA sulfurtransferase/glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (30/21); new base C (3/2); total (33/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01 | |||||||||||
CGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGCAAACGCCCCCTTTTA > NC_002947/5751602‑5751892 | cgcgAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCgg < 6:330746/149‑1 (MQ=255)cgcgAATTATATCGGAAAATGCTCAGGATTTGACCAACTTTTGATCAGAAGCTGTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCgg < 3:315122/149‑1 (MQ=255) gaaagtgcacccttacgatgcgttttttgatcgtgtcgcaccaatagggtgcgtggcgtgagtggtgtGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGTTGCAAAGCCCGTAGGAGCGGGt > 7:59538‑M1/69‑149 (MQ=255) gAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCTTTGCACCGCCTGTAGGCGCGGGtt < 2:318112/149‑1 (MQ=25) gggcacccagacgatgcgttttttgatcgtgtcgcaccaatagggtgcgtggcgtgagtggtctGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTAc > 8:360798‑M1/65‑149 (MQ=255) tCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCcgc > 3:119440/1‑148 (MQ=255) gAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACCGGCGGTGCAAAGCCCGTATGAGCGGGTTTACCcgcg > 1:23914/1‑146 (MQ=255) aTTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAgg < 2:119216/149‑1 (MQ=255) ttccgcAGGATTTGACCAACTTTTGATCAGCAGCTTTCAGATAAATCCAGAGGCCAGGTTTTGCACCGCCTGTCCCGGCCCTTTCGCGGGTAACCCCGCGCCTACCGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGc < 8:119685/144‑1 (MQ=37) ttGCTCAGGATTTGCCAAACTTTAGATCAGACTCGTTGAGATAAATGCTCAGGCCAGGTCTTGCCGCCCCTGTGCCGTCCCTTGCGCGGGTAAACCCGCGCCTACAGGCGGTGCAATGCCCGTAGCAGCGGGTTTACCCGCGAAGAGGc < 8:66484/149‑1 (MQ=25) tCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTAccc < 4:286769/135‑1 (MQ=255) tCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTAccc > 3:286769/1‑135 (MQ=255) cAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGccc > 5:311226/1‑146 (MQ=255) cAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGccc > 2:26638/1‑146 (MQ=255) gACCAAGTTTTGATCATAAGATTTGAGAAAATTGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCTTTTCGCGGTTAACCCCGGGCCTACTGGCGGTGAAAAGCCGGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTgg < 2:376513/148‑1 (MQ=37) gACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTgg < 1:26638/148‑1 (MQ=255) gACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTAAAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCAAGAACCTgg > 1:376513/1‑148 (MQ=255) aCCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTTAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGa > 1:123267/1‑113 (MQ=255) aCCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGa < 2:123267/113‑1 (MQ=255) cAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGcaca < 5:26072/149‑1 (MQ=255) aGAAGCTTTTATATAAATGCAGAGGCCAGGTGTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGGGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTcacgcc < 8:112419/148‑1 (MQ=255) gAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTcacgccac > 4:183561/1‑149 (MQ=255) aaGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCAcacca > 8:319514/1‑138 (MQ=255) aaGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCAcacca < 7:319514/138‑1 (MQ=255) ttGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCTTTGCACCGCCTGTAGGCGCggg > 6:275870/1‑97 (MQ=18) ttGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCTCCTACGGGCTTTGCACCGCCTGTAGGCGCggg < 5:275870/97‑1 (MQ=18) ttGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCCCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGGTTTCCCGCGAAGAGGCCCTAGAACCTGGCCAACCCCTCACgccccgcccc > 4:364814/1‑148 (MQ=38) tGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCAccc < 6:171573/148‑1 (MQ=255) gagaTAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCAcccaa > 3:351054/1‑147 (MQ=255) aaaTGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGt > 8:365601/1‑149 (MQ=255) aTGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGc < 4:351054/149‑1 (MQ=255) cAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGa > 1:1143/1‑148 (MQ=255) agagGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAAACCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGAGGGTTTACCCGCGCAGAGGCCCGATACCCTGGCACACCACTCCCGCCACGCCCCCTATTGGTGCGaca > 3:41122/1‑149 (MQ=37) agGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGacac > 6:275918/1‑148 (MQ=255) tGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAAc < 3:183561/148‑1 (MQ=255) cAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCa > 7:89101/1‑149 (MQ=255) aGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACCCCCCTCACGCCCCGCACCCTATTGGTGCGACACGATCAAAAAACGCAt > 1:144650/1‑149 (MQ=255) aGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCGATTGGTGCGACACGATCAAAAAACGCAt > 2:94260/1‑149 (MQ=255) cTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACACGCCGTGCAACCCCCGTAGGAGCCGGTTAACCCGCGAAGAGGCCCGACCACCTCCCACCCCACGCCCGCCCCGCACCCAAATGGTGCGCCCCGCTCAAAAAACGCCTCGTaa > 8:167669/1‑149 (MQ=16) ttCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAGACGCATCGTAAgg < 4:119440/148‑1 (MQ=255) cGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTg < 7:365601/148‑1 (MQ=255) ccTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACtt < 2:23914/149‑1 (MQ=255) cgGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCg > 3:341426/1‑149 (MQ=255) cccGCTCCTACGGGCTTTGCACCGCCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAAccc > 2:124658/1‑148 (MQ=37) ccGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTAATGGTGCGACACGATCAAAAAACCCATCGTAAGGGACCACTTACACCCGCTAACCCa > 7:145349/1‑148 (MQ=255) cgctCCTACGGGCTTTGCACCGCCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTAACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAAc > 2:389059/1‑149 (MQ=37) gacTACAGGCGGTGCAAAGACGGTAGTAGCGGGTTTACACGAGAAGAGGCAATAGAACCTGGCACACCACTCACGACACGCACCCTATTGGAGCGACACGATCAAAAAACTCATCGTAAGGGTGAACTTTCAAACGCTAACCCAgcgc > 8:331630/3‑148 (MQ=255) cTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCAt < 3:178099/112‑1 (MQ=255) cTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCAt > 4:178099/1‑112 (MQ=255) gCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCAcc < 6:55235/76‑1 (MQ=255) gCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCAcc > 5:55235/1‑76 (MQ=255) gCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCAc > 5:36168/1‑149 (MQ=255) gTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGc > 1:180336/1‑148 (MQ=255) cAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTATAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGCAAAc < 5:275918/149‑1 (MQ=255) cAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGCAAAc > 3:361308/1‑149 (MQ=255) aaaGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGCAAACg > 3:300641/1‑149 (MQ=255) cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGCAAACGcccc > 2:310113/1‑149 (MQ=255) ggAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGCAAACGCCCCCTTTTa < 4:341426/149‑1 (MQ=255) | CGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGCCAAATGCACGCAAACGCCCCCTTTTA > NC_002947/5751602‑5751892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |