| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,756,358 | T→A | 10.9% | intergenic (+35/‑653) | glnG → / → PP_5050 | two‑component system DNA‑binding response regulator GlnL/GlnG/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,756,358 | 0 | T | A | 10.9% | 111.1 / 6.4 | 46 | intergenic (+35/‑653) | glnG/PP_5050 | two‑component system DNA‑binding response regulator GlnL/GlnG/transposase |
| Reads supporting (aligned to +/- strand): ref base T (16/24); new base A (2/3); total (19/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
CGCGATGCTGCGCTGTTGCTGGGTTGGGGGCGCAATACCCTGACGCGCAAGATCAAGGAGCTGGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCCTGCTGGCCAATCTCCACCTGCC > NC_002947/5756213‑5756499 | cgcgATGCTGCGCTGTTGCTGGGTTGGGGGCGCAATACCCTGACGCGCAAGATCAAGGAGCTGGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGCCCACTGAGCTAGGAATTAGCTTTTTAGATTCCTGGGGCTGCTTTg > 7:59903/1‑149 (MQ=255) gcgATGCTGCGCTGTTGCTGGGTTGGGGGCGCAATACCCTGACGCGCAAGATCAAGGAGCTGGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCCCTGAGCTCTGAATTAGCTTGTTAGATAGCTGGGGCTGCtgtgg > 8:346717/1‑148 (MQ=255) ggATGCGGCGCGGGTGCAGGGTGGGGGTCCCAACCACCGCCCGCGCCAGATCAAGCACCTGTGGCTGACTGTGTCCGGGGTGGCTGATGAGGCAGGTGATGACCACTGAGCGCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCa < 8:170939/148‑1 (MQ=255) gATGCTCCCCTGTTGCGGGGTTGCGGGCCCCATCCCCTCACCCGCAAGATCCAGGCGCGGCGGCTGACGCGCGCCGCGGGGGATGATGAGGAAGGCGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAg < 4:165089/149‑1 (MQ=255) ctgcGCGGTTGCGGGGTTGGGGCCGCAATCCCCTGCCCCCCAAGATCACGCCGCGGGGCATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCAAAGCAGcccc < 6:340938/149‑2 (MQ=255) gTTGCTGGGTTGGGGGCGCAATACCCTGACGCGCAAGATCAAGGAGCTGGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGac < 8:257072/149‑1 (MQ=255) tGGGTTGGGGGCGCAATACCCTGACGCGCAAGATCAAGGAGCTGGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGCGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAg > 5:304280/1‑149 (MQ=255) gggTTGGGGGCGCAATACCCTGACGCGCAAGATCAAGGAGCTGGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAgg < 2:91868/149‑1 (MQ=255) gggggCGCAATACCCTGACGCGCAAGATCAAGGAGCTGGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCt < 2:118854/149‑1 (MQ=255) gcgcAATACCCTGACGCGCAAGATCAAGGAGCTCGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGATGGGGCTGCTTTGCAGCCCATCGCGCCACAAGGCCGCTACca > 2:108015/1‑149 (MQ=255) cgcacaaCCCTGACGCGCACGATCACGGTGCGGGGCACGAATGTGGCCCGGCGGCATGAGGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCcac < 1:108015/143‑1 (MQ=37) cccAATACCCCGACGGGCCTGATCAAGGAGCTGGGTCTGAATCTGGCCGGGGGGGTTGATGAGGAAGGTGATGACCACTTAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAATGCCGCTCCcac < 4:339397/147‑1 (MQ=37) aaTACCCAGCCGCCCAAGACCAACGACCTGGCGAACAATCTCCCCGGCGCGGACGATGAGGAAGCTCATGACCACTGAGCTCTGAATAAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAACGCCGCTCCcacacg < 1:76047/149‑3 (MQ=19) cgcgCAAGCTCAAGCCGCTGGGGAGGCATGCGGCCGGGGGGTATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTg < 6:296626/149‑1 (MQ=38) cgccCAAGCTCCACGCGCTGGGGATGAAGGGGGCCGGGGGGGAGGATGCGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTg < 2:253836/149‑1 (MQ=38) cAAGATCAAGGAGCTGGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGcccc > 7:278401/1‑149 (MQ=255) aaGATCAAGGAGCTGGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGcccc > 8:288553/1‑148 (MQ=255) gggATGAATGTGGCCGGGGGGGATGATGTGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTg > 1:222537/1‑149 (MQ=255) gAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAATGCCGCTCACACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGaca > 7:39372/1‑148 (MQ=255) tgtgGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGAcacaca > 4:111734/1‑149 (MQ=255) ggggCCGGGGGCGACGATGAGGAAGGGGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCGGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACAt < 8:59903/147‑1 (MQ=255) ggCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATcg < 6:192434/149‑1 (MQ=255) ccGGGGGGTATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTACATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATcgcg < 7:274579/149‑1 (MQ=255) gaGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTcc > 4:301019/1‑86 (MQ=255) gaGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTcc < 3:301019/86‑1 (MQ=255) aaGGTGATGCCCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGt < 3:15426/149‑1 (MQ=255) aGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGtt < 4:165975/149‑1 (MQ=255) ggTGATGCCCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCGTTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAGGATCACCTCTGCCCACAAGGCTGATGCGGTGGGCACGCATCGCGGTCGTCGTAGGAGCGGGtt > 8:15104/1‑148 (MQ=38) gTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGgtc < 5:199144/131‑1 (MQ=255) gTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGgtc > 6:199144/1‑131 (MQ=255) gTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCa < 2:222537/149‑1 (MQ=255) aGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGc > 7:220573/1‑149 (MQ=255) ctctGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCg > 5:387110/1‑149 (MQ=255) tctGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCgg > 2:180005/1‑149 (MQ=255) ttAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCAc < 1:161954/128‑1 (MQ=255) ttAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCctgc < 8:278401/149‑1 (MQ=255) ttAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCAGATGCGGTGGACACACATCGCGCTCGTCGTAGGACCCGGTTCAc > 2:161954/1‑128 (MQ=255) tAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGgtcgtc < 6:80465/103‑1 (MQ=255) tAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGgtcgtc > 5:80465/1‑103 (MQ=255) aGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCCTGCTGGCCAAtc < 6:387110/148‑1 (MQ=255) gATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGGTGATGCGGTGGGCACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCCTGCTGGCCAAtct < 7:15104/148‑1 (MQ=255) gatgggCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCCTGCTGGCCAAtc > 8:76494/4‑142 (MQ=255) gatgggCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCCTGCTGGCCAAtc < 7:76494/139‑1 (MQ=255) cTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCCTGCTGGCCAATCTCCACCt > 8:211571/1‑149 (MQ=255) tgggCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCCTGCTGGCCAAt < 6:141839/138‑1 (MQ=255) tgggCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCCTGCTGGCCAAt > 5:141839/2‑139 (MQ=255) gggCTGCTTTGCAGCCCAGCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCCTGCTGGCCAATCTCCACCTGcc < 7:346717/149‑1 (MQ=255) | CGCGATGCTGCGCTGTTGCTGGGTTGGGGGCGCAATACCCTGACGCGCAAGATCAAGGAGCTGGGGATGAATGTGGCCGGGGGGGATGATGAGGAAGGTGATGACCACTGAGCTCTGAATTAGCTTTTTAGATTGCTGGGGCTGCTTTGCAGCCCATCGCGACACAAGGCCGCTCCCACAGGATCACCTCTGCCCCCAAGGCTGATGCGGTGGACACACATCGCGGTCGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCCTGCTGGCCAATCTCCACCTGCC > NC_002947/5756213‑5756499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |