Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,824,715 C→A 6.3% intergenic (+139/+35) PP_5102 → / ← trmB hypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,824,7150CA 6.3% 243.9 / 5.8 79intergenic (+139/+35)PP_5102/trmBhypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB
Reads supporting (aligned to +/- strand):  ref base C (39/35);  new base A (3/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

CCGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCC  >  NC_002947/5824567‑5824857
                                                                                                                                                    |                                                                                                                                              
ccgcGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTc                                                                                                                                                <  3:306292/149‑1 (MQ=255)
   cgGCTGAGCGAATCACCCGTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCGACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAgg                                                                                                                                             <  8:338098/149‑1 (MQ=255)
      cTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCg                                                                                                                                          <  5:28886/149‑1 (MQ=255)
            gAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTa                                                                                                                                    >  5:75532/1‑149 (MQ=255)
             aaTGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAc                                                                                                                                   <  4:196457/149‑1 (MQ=255)
                   cccTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGaa                                                                                                                               >  3:68779/1‑147 (MQ=255)
                     cTTGTAGGAGCGGATTCATCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAgg                                                                                                                           <  1:284936/149‑1 (MQ=255)
                     cTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAgg                                                                                                                           >  1:174246/1‑149 (MQ=255)
                     cTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAgg                                                                                                                           >  4:312444/1‑149 (MQ=255)
                        gTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGcc                                                                                                                         <  1:40205/148‑1 (MQ=255)
                        gTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGcc                                                                                                                         <  8:21869/148‑1 (MQ=255)
                           ggAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAcc                                                                                                                                  <  5:205857/136‑1 (MQ=255)
                           ggAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAcc                                                                                                                                  >  6:205857/1‑136 (MQ=255)
                              gCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATAAg                                                                                                                  >  2:299397/1‑149 (MQ=255)
                               cGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGc                                                                                                                 <  6:75532/149‑1 (MQ=255)
                                   ttCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGc                                                                                                              <  8:259385/148‑1 (MQ=255)
                                    tCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCt                                                                                                             >  8:7918/1‑148 (MQ=255)
                                      aaCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCaa                                                                                                          >  2:301739/1‑149 (MQ=255)
                                      aaCCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCa                                                                                                           >  7:163666/1‑148 (MQ=255)
                                         cgcgAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATcc                                                                                                       <  3:312444/149‑1 (MQ=255)
                                          gcgAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCa                                                                                                      >  8:225503/1‑149 (MQ=255)
                                           cgAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCAc                                                                                                     >  5:136973/1‑149 (MQ=255)
                                           cgAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCAc                                                                                                     <  2:174246/149‑1 (MQ=255)
                                            gAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCAcc                                                                                                    >  3:65199/1‑149 (MQ=255)
                                              aaGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCtt                                                                                                  >  6:10643/1‑149 (MQ=255)
                                               aGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTtc                                                                                                 >  7:301899/1‑149 (MQ=255)
                                                    cggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGaa                                                                                            <  3:360779/149‑1 (MQ=255)
                                                     ggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAAc                                                                                           <  2:15502/149‑1 (MQ=255)
                                                        ccTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACtt                                                                                         >  5:152511/1‑148 (MQ=255)
                                                        ccTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACtt                                                                                         >  3:147400/1‑148 (MQ=255)
                                                        ccTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTc                                                                                        <  7:7918/149‑1 (MQ=255)
                                                           gctgGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGtt                                                                                                                                       <  6:49919/99‑1 (MQ=255)
                                                           gctgGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGtt                                                                                                                                       >  5:49919/1‑99 (MQ=255)
                                                               gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTccc                                                                                 >  5:13458/1‑149 (MQ=255)
                                                               gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTccc                                                                                 <  4:65199/149‑1 (MQ=255)
                                                               gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTcc                                                                                  <  7:108996/148‑1 (MQ=255)
                                                                 cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCcac                                                                               >  3:218389/1‑149 (MQ=255)
                                                                      tgcACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACAcccc                                                                          >  8:255110/3‑149 (MQ=255)
                                                                      tcCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACAGAAAAGGCCGTATCAGCCCCACAATCCACCTACACGAAATTCAAGTCCCACAaccc                                                                          >  5:126581/1‑149 (MQ=37)
                                                                       ccACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCg                                                                         >  1:105296/1‑149 (MQ=255)
                                                                            cGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGATCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCAGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGccc                                                                    >  6:174496/1‑149 (MQ=255)
                                                                                 ttCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGcc                                                               >  3:257696/1‑149 (MQ=255)
                                                                                  tCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACCCCCCGTGCCCccgccg                                                              >  6:138006/1‑149 (MQ=255)
                                                                                          ggCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACcgc                                                      >  3:295917/1‑149 (MQ=255)
                                                                                           gcgcCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACcgcg                                                     <  3:138750/149‑1 (MQ=255)
                                                                                                       tATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCATGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACtt                                         <  1:250273/149‑1 (MQ=255)
                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTgg                                       <  4:147400/149‑1 (MQ=255)
                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTgg                                       >  6:39005/1‑149 (MQ=255)
                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTgg                                       <  1:301739/149‑1 (MQ=255)
                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTg                                        <  7:225503/148‑1 (MQ=255)
                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTg                                        <  4:257696/148‑1 (MQ=255)
                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTg                                        <  4:218389/148‑1 (MQ=255)
                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTg                                        <  8:301899/148‑1 (MQ=255)
                                                                                                           cgcgGTTGAATCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGt                                      <  1:160122/148‑1 (MQ=255)
                                                                                                               ggTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCg                                 >  2:97405/1‑149 (MQ=255)
                                                                                                                gTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCgg                                >  8:254886/1‑149 (MQ=255)
                                                                                                                   aaCCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGgcg                             <  6:136973/149‑1 (MQ=255)
                                                                                                                   aaCCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGgc                              <  5:10643/148‑1 (MQ=255)
                                                                                                                      ccGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGctc                          >  2:68417/1‑149 (MQ=255)
                                                                                                                            cTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCg                      <  4:68779/147‑1 (MQ=255)
                                                                                                                                aaaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTGGAACTTGGTGCTCGGGCGCTCTTCGGGgcgc                >  4:19584/1‑149 (MQ=255)
                                                                                                                                aaaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcgc                >  4:36171/1‑149 (MQ=255)
                                                                                                                                aaaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcgc                >  2:98071/1‑149 (MQ=255)
                                                                                                                                aaaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACAACCCGTGCCCCAGCCGCTCACCTCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGtcgc                >  4:77716/1‑149 (MQ=255)
                                                                                                                                 aaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGcgcg               <  7:254886/149‑1 (MQ=255)
                                                                                                                                  aGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCgg              >  7:275236/1‑149 (MQ=255)
                                                                                                                                   ggtaaaaCCGCCCTGAAAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCg                                              <  1:350237/113‑1 (MQ=39)
                                                                                                                                   ggtaaaaCCGCCCTGAAAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCg                                              >  2:350237/4‑116 (MQ=39)
                                                                                                                                   gAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCTGGGCGCGGc             <  5:174496/149‑1 (MQ=255)
                                                                                                                                    aaaaaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGca            >  1:83578/1‑149 (MQ=255)
                                                                                                                                       aaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACAt         <  8:163666/149‑1 (MQ=255)
                                                                                                                                        aaCCGCCCTGAAAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCgg              >  6:52956/1‑143 (MQ=255)
                                                                                                                                        aaCCGCCCTGAAAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCgg              <  5:52956/143‑1 (MQ=255)
                                                                                                                                        aaCCGCCCTGAAAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCCGGCGCTCTTCGGGGCGCGGcaca          >  8:218666/1‑147 (MQ=255)
                                                                                                                                         aCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAg       <  6:126581/149‑1 (MQ=255)
                                                                                                                                             cccTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGc   <  4:295917/149‑1 (MQ=255)
                                                                                                                                              ccTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGt     <  2:368457/146‑1 (MQ=255)
                                                                                                                                              ccTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGt     >  1:368457/1‑146 (MQ=255)
                                                                                                                                              ccTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGcc  >  8:140250/1‑149 (MQ=255)
                                                                                                                                              ccTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGcc  >  5:362845/1‑149 (MQ=255)
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CCGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCC  >  NC_002947/5824567‑5824857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑