Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,856,909	C→T	6.6%	A279V (GCC→GTC)	PP_5133 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,856,909	0	C	T	6.6%	176.3 / 2.5	60	A279V (GCC→GTC)	PP_5133	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (27/29);  new base T (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

GTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGATGCTGCCCCGCAAGCCTTGCCACAG  >  NC_002947/5856763‑5857057                                                                                                                                                    |                                                                                                                                                     gtTGCTGGGCTGCGCGGTGCCCTACGAAGCAATGAGCCGCGCCAGCGGCGACAGCCTGCCAG‑CCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCgg                                                                                                                                                   <  6:165046/149‑1 (MQ=255)  ttGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCgg                                                                                                                                                   >  6:313712/1‑149 (MQ=255)      tGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTa                                                                                                                                               >  8:102670/1‑149 (MQ=255)         gCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGc                                                                                                                                            <  1:344538/149‑1 (MQ=255)         gCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGc                                                                                                                                            <  4:217664/149‑1 (MQ=255)          cTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGcc                                                                                                                                           >  1:196656/1‑149 (MQ=255)             cGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCt                                                                                                                                          <  5:379940/147‑1 (MQ=255)                cGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGTCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGTCGGGTTATGCCTGtggc                                                                                                                                     >  7:166332/1‑149 (MQ=255)                           aaGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAg                                                                                                                          <  7:237688/149‑1 (MQ=255)                             gCCATGATCCGCGCCAGCGGCGACAGCCTGCGAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAggg                                                                                                                        <  5:312454/149‑1 (MQ=255)                               cATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGcc                                                                                                                      >  8:8597/1‑149 (MQ=255)                                       gcgcCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGtggc                                                                                                                                     <  1:48310/126‑1 (MQ=255)                                       gcgcCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGtggc                                                                                                                                     >  2:48310/1‑126 (MQ=255)                                       gcgcCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGc                                                                                                              <  3:177714/149‑1 (MQ=255)                                       gcgcCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGc                                                                                                              <  2:159415/149‑1 (MQ=255)                                          ccAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCt                                                                                                            >  8:320841/1‑148 (MQ=255)                                                  gACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGa                                                                                                   <  7:320841/149‑1 (MQ=255)                                                        cTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTggg                                                                                             <  5:276060/149‑1 (MQ=255)                                                               cccTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTggg                                                                                             <  7:143146/142‑1 (MQ=255)                                                               cccTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTggg                                                                                             >  8:143146/1‑142 (MQ=255)                                                               ccagggACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCGGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCGGGACCTGGGCCTGccc                                                                                      <  7:166401/145‑1 (MQ=255)                                                                 cTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGTCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCgg                                                                                    >  8:22615/1‑149 (MQ=255)                                                                 cTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCgg                                                                                    <  2:199856/149‑1 (MQ=255)                                                                    gACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGc                                                                                   <  2:14432/147‑1 (MQ=255)                                                                    gACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTa                                                                                 >  5:7/1‑149 (MQ=255)                                                                        tGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGTCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTAcgcg                                                                             <  7:22615/149‑1 (MQ=255)                                                                         ggCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGACCGCCTGACGCTGGACCTGGGCCTGCCCGGCTAcgcg                                                                             >  2:260867/1‑148 (MQ=255)                                                                         ggCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACgcgc                                                                            <  7:8597/149‑1 (MQ=255)                                                                             cGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACt                                                                        <  5:50477/149‑1 (MQ=255)                                                                                    ccTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAg                                                                 <  7:191779/149‑1 (MQ=255)                                                                                     cTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTGACCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAg                                                                 >  1:85730/1‑149 (MQ=255)                                                                                     cTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAgg                                                                >  6:235055/1‑149 (MQ=255)                                                                                      tGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAgg                                                                >  5:255582/1‑148 (MQ=255)                                                                                        ggCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCAc                                                             <  2:196656/149‑1 (MQ=255)                                                                                        ggCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCCTCGCGCAGTTGcgcgtagccg                                                             <  4:222249/149‑7 (MQ=255)                                                                                          ccAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGTCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTg                                                           <  8:166332/149‑1 (MQ=255)                                                                                          ccAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACt                                                            >  6:119359/1‑148 (MQ=255)                                                                                           cAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGa                                                          >  4:74998/1‑149 (MQ=255)                                                                                              ctggctgCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGc                                                       >  6:185260/1‑149 (MQ=255)                                                                                                     ctCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCgcgc                                                  <  7:102670/147‑1 (MQ=255)                                                                                                         gCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCagttgc                                            <  6:7/149‑5 (MQ=255)                                                                                                               tgCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATc                                      >  3:312321/1‑149 (MQ=255)                                                                                                               tgCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATc                                      <  2:19803/149‑1 (MQ=255)                                                                                                                 cTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGa                                                                  <  6:259795/119‑1 (MQ=255)                                                                                                                  tGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGc                                   >  5:53237/1‑149 (MQ=255)                                                                                                                   ggTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCg                                  >  5:49186/1‑149 (MQ=255)                                                                                                                   ggTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCg                                  >  7:27394/1‑149 (MQ=255)                                                                                                                        aCGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAg                             >  4:146747/1‑149 (MQ=255)                                                                                                                         cGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAg                             >  4:44754/1‑148 (MQ=255)                                                                                                                          ggCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGGGAGCGCATCGGCGTGCAGGa                           >  4:279101/1‑149 (MQ=255)                                                                                                                           gCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGAt                          <  6:255582/149‑1 (MQ=255)                                                                                                                            ccTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACACTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGAtg                         >  8:300184/1‑149 (MQ=255)                                                                                                                              tgctg‑cCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGAtgct                       >  6:291106/1‑149 (MQ=255)                                                                                                                                      ccGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGATGCTGCCCCg                 >  6:200524/1‑148 (MQ=255)                                                                                                                                         ccTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGATGCTGCCCCGCAAg             <  7:330337/149‑1 (MQ=255)                                                                                                                                             ctgctgGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGATGCTGCCCCGCAAGCCtt         <  5:185260/149‑1 (MQ=255)                                                                                                                                                ctgGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGATGCTGCCCCGCAAGc            <  6:255691/143‑1 (MQ=255)                                                                                                                                                ctgGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGATGCTGCCCCGCAAGc            >  5:255691/1‑143 (MQ=255)                                                                                                                                                ctgGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGATGCTGCCCCGCAAGCCTTGc       <  8:27394/148‑1 (MQ=255)                                                                                                                                                   gCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGATGCTGCCCCGCAAGCCTTGCca     >  7:270752/1‑147 (MQ=255)                                                                                                                                                    ccGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGATGCTGCCCCGCAAGCCTTGCCACAg  <  5:313712/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GTTGCTGGGCTTCGCCGTGCCCGACGAAGCCATGATCCGCGCCAGCGGCGACAGCCTGCCAGCCCTGGACGTGGCCCGCCAGGCCTGGGCCAGCTGGCTGCTCGGCGTGGTGCTGGTATACGGCCTGCTG‑CCCCGCCTGCTGCTGGCCGGGTTATGCCTGTGGCGCTGGCGCCAGGGCCGTGAACGCCTGACGCTGGACCTGGGCCTGCCCGGCTACGCGCAACTGCGCGAGGCACTGATGCCGCGCAGCGAGCGCATCGGCGTGCAGGATGCTGCCCCGCAAGCCTTGCCACAG  >  NC_002947/5856763‑5857057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑