Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,857,961	T→A	7.6%	L170L (CTT→CTA) ‡	PP_5134 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,857,961	0	T	A	7.6%	235.0 / 7.9	79	L170L (CTT→CTA) ‡	PP_5134	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (35/38);  new base A (3/3);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

GCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAG  >  NC_002947/5857813‑5858108                                                                                                                                                       |                                                                                                                                                    gCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCtt                                                                                                                                                     <  1:184582/149‑1 (MQ=255) gCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCtt                                                                                                                                                     >  5:302533/1‑149 (MQ=255)      tATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGccc                                                                                                                                                 <  8:80479/148‑1 (MQ=255)            ttCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGAGGAACTGGAAGTGCTGGCCTACTGCGGCAAGCCGTTGGTGCCGGTGGTCAACTTCGTCGCCAGCAGCCAG‑GA‑CGGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTgg                                                                                                                                          <  7:224218/148‑1 (MQ=255)                aTGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGGGCAAGGGCtt                                                                                                                                      <  3:193084/149‑1 (MQ=255)                 tGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTc                                                                                                                                     <  1:208125/149‑1 (MQ=255)                   cgcgcgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGGGCAAGGGCTTCAc                                                                                                                                   <  5:38015/149‑1 (MQ=255)                       cgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCAt                                                                                                                               >  8:234222/1‑149 (MQ=255)                         aGCCGGGGCTGGCCAAGTACCGCGTCGAACTGGAAGGGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCATCAGCCAG‑CATCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCAtt                                                                                                                              <  1:290507/149‑1 (MQ=255)                                  tGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTc                                                                                                                    >  6:42487/1‑149 (MQ=255)                                        agcaCCGCGACGAACTGGAAGGGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGc                                                                                                              <  3:278589/146‑1 (MQ=255)                                            ccGCTACGAACTGTAAGTGCTGGCCAAGTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCAAGAAGCATGCCT‑TGGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTg                                                                                                           <  1:314816/149‑1 (MQ=255)                                            ccGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACg                                                                                                                        <  2:283455/135‑1 (MQ=255)                                            ccGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACg                                                                                                                        >  1:283455/1‑135 (MQ=255)                                            ccGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTgg                                                                                                          >  3:371182/1‑149 (MQ=255)                                               cgacgaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGccc                                                                                                       >  1:268811/1‑149 (MQ=255)                                                gacgaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGcccc                                                                                                      <  7:40519/149‑1 (MQ=255)                                                    aaCTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                    >  1:159856/1‑147 (MQ=255)                                                     aCTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                    >  5:263531/1‑146 (MQ=255)                                                     aCTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                    >  3:301225/1‑146 (MQ=255)                                                      cTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCa                                                                                                  >  7:167800/1‑147 (MQ=255)                                                      cTGGAAGGGCGGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCaga                                                                                                <  5:35386/149‑1 (MQ=255)                                                       tGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTg                                                                                                                                           <  6:303693/105‑1 (MQ=255)                                                       tGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTg                                                                                                                                           >  5:303693/1‑105 (MQ=255)                                                         gAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGTTACGGTTCGACAGCGTGGCCCCGCCagag                                                                                               <  2:315593/147‑1 (MQ=255)                                                         gAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCagag                                                                                               <  4:301225/147‑1 (MQ=255)                                                            gTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGGCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATgg                                                                                          <  7:217863/149‑1 (MQ=255)                                                             tGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                         <  6:263531/149‑1 (MQ=255)                                                               cTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGtt                                                                                                                     >  1:167756/1‑119 (MQ=255)                                                               cTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGtt                                                                                                                     <  2:167756/119‑1 (MQ=255)                                                               cTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                         <  6:271312/147‑1 (MQ=255)                                                                  gCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAgcg                                                                                    >  3:207137/1‑149 (MQ=255)                                                                  gCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAgcg                                                                                    >  5:300169/1‑149 (MQ=255)                                                                    cAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGcc                                                                                  >  7:346681/1‑149 (MQ=255)                                                                    cAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGcc                                                                                  >  1:29928/1‑149 (MQ=255)                                                                    cAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGcc                                                                                  >  6:359144/1‑149 (MQ=255)                                                                          cGGCATGCCTTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑GCGCGAGCTTGAATGGCGTGAAGCCCTTGCCCGTCTTGGGCTTCACGCTTTGGTACGGTTCGACAGCTTGTCCCCGCCAGATGATGGTGAGCGCGGttt                                                                              <  8:177826/147‑1 (MQ=255)                                                                          cGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTg                                                                             >  6:93750/1‑148 (MQ=255)                                                                          cGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGt                                                                            <  2:126483/149‑1 (MQ=255)                                                                           ggCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTa                                                                           >  3:292953/1‑149 (MQ=255)                                                                           ggCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTa                                                                           >  5:357870/1‑149 (MQ=255)                                                                            gCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTAc                                                                          >  2:59695/1‑149 (MQ=255)                                                                            gCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTAc                                                                          >  6:183849/1‑149 (MQ=255)                                                                             cAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACg                                                                         <  7:234222/149‑1 (MQ=255)                                                                              aaGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                    <  8:18642/121‑1 (MQ=255)                                                                              aaGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                    >  7:18642/1‑121 (MQ=255)                                                                              aaGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACg                                                                         >  2:338780/1‑148 (MQ=255)                                                                                   gTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTg                                                                                        >  3:110307/1‑128 (MQ=255)                                                                                   gTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTg                                                                                        <  4:110307/128‑1 (MQ=255)                                                                                       ctgcCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTgg                                                                <  5:42487/148‑1 (MQ=255)                                                                                       ctgcCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTgg                                                                <  5:359144/148‑1 (MQ=255)                                                                                       ctgcCGGTGCTCAACTTCGGCGCCACCCGCCAG‑CC‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGgc                                                               <  8:346681/149‑1 (MQ=255)                                                                                          ccGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgct                                                            >  5:170478/1‑149 (MQ=255)                                                                                          ccGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCAGTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgct                                                            <  8:167800/149‑1 (MQ=255)                                                                                            ggTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgctgc                                                          <  1:338780/149‑1 (MQ=255)                                                                                                cTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgctgctgct                                                      >  5:219214/1‑149 (MQ=255)                                                                                                 tCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                         >  7:288021/1‑113 (MQ=255)                                                                                                 tCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                         <  8:288021/113‑1 (MQ=255)                                                                                                   aaCTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGctgctgctg                                                     <  4:207137/147‑1 (MQ=255)                                                                                                   aaCTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGctgctgctg                                                     <  7:283447/147‑1 (MQ=255)                                                                                                   aaCTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGACCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGCCAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGGTTGTACGAAAGCCTGCCGCTGCTGCTgg                                                    >  3:69506/1‑148 (MQ=255)                                                                                                         gtcgCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgc                                             >  5:251476/1‑149 (MQ=255)                                                                                                                gcagccag‑ca‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGcccgcccg                                      <  2:159856/149‑1 (MQ=255)                                                                                                                  agccag‑ca‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGgc                                    <  8:178708/149‑1 (MQ=255)                                                                                                                          a‑CCGCCAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACCGATCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGCAAAACGCCCGCCCGgcgc                                  >  5:285154/1‑144 (MQ=255)                                                                                                                            ccGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAAc                            <  4:7849/149‑1 (MQ=255)                                                                                                                             cgcgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACg                           >  6:84339/1‑149 (MQ=255)                                                                                                                              gcgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACgg                          >  7:351772/1‑149 (MQ=255)                                                                                                                               cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGc                         <  6:219214/149‑1 (MQ=255)                                                                                                                               cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGc                         <  5:93750/149‑1 (MQ=255)                                                                                                                               cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCAGTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGc                         <  8:351772/149‑1 (MQ=255)                                                                                                                                 aGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCt                        >  6:222408/1‑148 (MQ=255)                                                                                                                                  gCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGa                      <  6:251476/149‑1 (MQ=255)                                                                                                                                       aaTGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATcga                  <  6:300169/148‑1 (MQ=255)                                                                                                                                            cGTGAAGCCCAAGCCGGGCAAGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCgacgac              >  2:115421/1‑147 (MQ=255)                                                                                                                                             gTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGAcccg           >  4:71735/1‑147 (MQ=255)                                                                                                                                             gTGAAGCCCAAGCCGGGCAAGGGCTTCACGCATTGGTACGGTTCGATAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACcag           >  3:37989/1‑149 (MQ=255)                                                                                                                                             gTGAAGCCCAAGCCGGGCAAGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCaa                             >  6:223653/1‑131 (MQ=255)                                                                                                                                             gTGAAGCCCAAGCCGGGCAAGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCaa                             <  5:223653/131‑1 (MQ=255)                                                                                                                                                   cccTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGca     <  6:302533/149‑1 (MQ=255)                                                                                                                                                     cTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGcaca   >  6:259611/1‑149 (MQ=255)                                                                                                                                                      ttGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAg  >  7:330187/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                                    GCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAG‑CA‑CCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAG  >  NC_002947/5857813‑5858108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑