Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 432,993 A→T 6.2% intergenic (+18/+29) PP_0355 → / ← glcB two‑component system response regulator/malate synthase G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947432,9930AT 6.2% 245.7 / 4.9 80intergenic (+18/+29)PP_0355/glcBtwo‑component system response regulator/malate synthase G
Reads supporting (aligned to +/- strand):  ref base A (33/42);  new base T (3/2);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

GGTGTCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCC  >  NC_002947/432846‑433136
                                                                                                                                                    |                                                                                                                                               
ggtgTCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTa                                                                                                                                                 >  4:368333/1‑148 (MQ=255)
  tgtCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTaaaa                                                                                                                                              <  7:156862/149‑1 (MQ=255)
    tCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACg                                                                                                                                            <  6:227445/149‑1 (MQ=255)
          gACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTTTTACGGTcccc                                                                                                                                      <  8:90263/149‑1 (MQ=255)
          gACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTTTTACGGTcccc                                                                                                                                      <  2:15969/149‑1 (MQ=255)
          gACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAACCGGTcccc                                                                                                                                      >  5:63298/1‑149 (MQ=255)
             acCGATGTGGAAGAGGCAGGGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTGGGACCGGGTGCACCAAGAACTGAAACTTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTcccccgg                                                                                                                                   <  5:266401/149‑3 (MQ=255)
                  tgtgGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGgcagc                                                                                                                              <  5:314146/149‑1 (MQ=255)
                  tgtgGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGgcagc                                                                                                                              <  7:176196/149‑1 (MQ=255)
                    tgGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGgcagca                                                                                                                             <  8:123588/148‑1 (MQ=255)
                      gAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTc                                                                                                                                         >  3:97769/1‑134 (MQ=255)
                      gAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTc                                                                                                                                         <  4:97769/134‑1 (MQ=255)
                      gAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGcagcag                                                                                                                            <  3:329194/147‑1 (MQ=255)
                      gAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGcagcag                                                                                                                            >  4:329194/1‑147 (MQ=255)
                      gAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAt                                                                                                                          <  6:24256/149‑1 (MQ=255)
                       aaGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAtg                                                                                                                         <  1:73498/149‑1 (MQ=255)
                               gTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTAc                                                                                                                 <  5:161966/149‑1 (MQ=255)
                                  gATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAAc                                                                                                              <  5:31783/149‑1 (MQ=255)
                                      tgATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGt                                                                                                          >  3:28663/1‑149 (MQ=255)
                                         tGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAGGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAc                                                                                                       >  3:295436/1‑149 (MQ=255)
                                          gccccTGGGGGTGGTGGAATTCCTGCTCAACCCGTTGGATGGGGGCAAGGTGTTGGAGCTGGTTAAAAAACAACTTAAAATTGGTTGATGC‑GCCAGGGGGACCGTAAACCGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAc                                                                                                       <  6:63298/145‑1 (MQ=255)
                                          gCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAcg                                                                                                      >  6:352192/1‑149 (MQ=255)
                                            aCCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAcgcg                                                                                                    >  2:76883/1‑149 (MQ=255)
                                            aCCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAcgc                                                                                                     <  7:198050/148‑1 (MQ=255)
                                            aCCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAcgc                                                                                                     >  8:198050/1‑148 (MQ=255)
                                             ccTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑ACCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACgcgc                                                                                                   >  2:169716/1‑149 (MQ=255)
                                                 ggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACg                                                                                                                                            <  5:333879/104‑1 (MQ=255)
                                                 ggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACg                                                                                                                                            >  6:333879/1‑104 (MQ=255)
                                                 ggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTgg                                                                                                                                  >  4:34227/1‑114 (MQ=255)
                                                 ggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTgg                                                                                                                                  <  3:34227/114‑1 (MQ=255)
                                                 ggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCtt                                                                                                <  2:311611/148‑1 (MQ=255)
                                                    gtggtgGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAAc                                                                                            <  4:352335/149‑1 (MQ=255)
                                                        tggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgc                                                                                        <  3:370701/149‑1 (MQ=255)
                                                        tggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgc                                                                                        >  1:79130/1‑149 (MQ=255)
                                                         ggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAATATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCtt                                                                                                >  2:261940/1‑140 (MQ=255)
                                                         ggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCtt                                                                                                <  1:261940/140‑1 (MQ=255)
                                                          gACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgcg                                                                                       <  4:309404/148‑1 (MQ=255)
                                                           aCTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCgcggc                                                                                     <  5:279210/149‑1 (MQ=255)
                                                            cTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgcg                                                                                       >  5:236658/1‑146 (MQ=255)
                                                               ccTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGAcg                                                                                 >  6:154717/1‑149 (MQ=255)
                                                                   cTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGAcggcgg                                                                             >  1:354166/1‑149 (MQ=255)
                                                                   cTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGAcggc                                                                               <  3:252259/147‑1 (MQ=255)
                                                                      aaGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTg                                                                           <  2:25503/148‑1 (MQ=255)
                                                                            gTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATCC‑GACAGGCGGACCGAAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTAcc                                                                    >  5:274777/1‑149 (MQ=255)
                                                                               gATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCgg                                                                  <  4:367563/148‑1 (MQ=255)
                                                                                 tCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTc                                                               >  1:389849/1‑149 (MQ=255)
                                                                                     ggCAAGCTGTTGGAGCTGGTGAACATAGAACTTAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACGCGTTACGCGCCTTGATCTCGCGGCGACGGCGGTGCAGTACCGGCTCGGt                                                            <  3:97926/148‑1 (MQ=255)
                                                                                     ggCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGta                                                           >  6:167094/1‑149 (MQ=255)
                                                                                        aaGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATaa                                                        <  3:368333/149‑1 (MQ=255)
                                                                                                    cTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAGCCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgc                                            >  8:65430/1‑149 (MQ=255)
                                                                                                       gTGATCAATGAACTGAAAATTTGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgctt                                          <  6:236658/148‑1 (MQ=255)
                                                                                                       gTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgcttg                                         >  6:4946/1‑149 (MQ=255)
                                                                                                       gTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgcttg                                         >  4:143600/1‑149 (MQ=255)
                                                                                                         gAACAAAGAACTGAAAATTGGATGATGCGGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGa                                                                                   >  1:28634/1‑106 (MQ=255)
                                                                                                         gAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGa                                                                                   <  2:28634/105‑1 (MQ=255)
                                                                                                            cAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCa                                      <  2:205987/147‑1 (MQ=255)
                                                                                                            cAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCa                                      >  1:205987/1‑147 (MQ=255)
                                                                                                              aaGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCtt                                  >  2:244487/1‑149 (MQ=255)
                                                                                                              aaGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCtt                                  <  3:143600/149‑1 (MQ=255)
                                                                                                               aGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTc                                 >  2:168348/1‑149 (MQ=255)
                                                                                                               aGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTc                                 >  3:100214/1‑149 (MQ=255)
                                                                                                                      aaaaTTGGATGATGC‑GCCATGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATTCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTAc                           <  6:191525/148‑1 (MQ=255)
                                                                                                                      aaaaTTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTAc                           <  5:154717/148‑1 (MQ=255)
                                                                                                                        aaTTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGcc                                       >  4:197545/1‑134 (MQ=255)
                                                                                                                        aaTTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGcc                                       <  3:197545/134‑1 (MQ=255)
                                                                                                                             gatgatGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCca                   <  4:28663/149‑1 (MQ=255)
                                                                                                                               tgatgC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCcaca                 <  1:168348/149‑1 (MQ=255)
                                                                                                                                 atgC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAg                <  4:295436/148‑1 (MQ=255)
                                                                                                                                 atgC‑ACCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAg                <  1:169716/148‑1 (MQ=255)
                                                                                                                                      gcCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAgccgcc           >  5:75141/1‑149 (MQ=255)
                                                                                                                                       ccAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCt          <  1:76883/149‑1 (MQ=255)
                                                                                                                                        cAGGGGGACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTg         >  2:128321/1‑149 (MQ=255)
                                                                                                                                        cAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTg         >  8:87676/1‑149 (MQ=255)
                                                                                                                                         aGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTg         >  6:363899/1‑148 (MQ=255)
                                                                                                                                          gggggACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTgg        >  6:229630/1‑148 (MQ=255)
                                                                                                                                           ggggACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGaa      >  2:83017/1‑149 (MQ=255)
                                                                                                                                           ggggACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGaa      <  2:354166/149‑1 (MQ=255)
                                                                                                                                             ggACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCa                                                                         >  4:71305/1‑80 (MQ=255)
                                                                                                                                             ggACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCa                                                                         <  3:71305/80‑1 (MQ=255)
                                                                                                                                               aCCGTAAAACGGGCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGcc  <  4:314038/149‑1 (MQ=255)
                                                                                                                                                 cGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGATATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCcaca                 <  2:207977/132‑1 (MQ=255)
                                                                                                                                                 cGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGATATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCcaca                 >  1:207977/1‑132 (MQ=255)
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GGTGTCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGC‑GCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCC  >  NC_002947/432846‑433136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑