Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,875,944	G→A	6.5%	A76V (GCC→GTC)	ilvA‑II ←	threonine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,875,944	0	G	A	6.5%	180.7 / 2.1	61	A76V (GCC→GTC)	ilvA‑II	threonine deaminase
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base A (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

GGGAACGAGTCGCCATGCAGCACCACCTTGCCACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGCCCAGGCGCTTGCTCAGCTGC  >  NC_002947/5875797‑5876090                                                                                                                                                    |                                                                                                                                                   gggAACGAGTCGCCATGCAGCACCACCTTGCCACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGc                                                                                                                                                   <  3:279657/149‑1 (MQ=255)   gAACGAGTCGCCATGCAGCACCACCTTGCCACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCgg                                                                                                                                                    >  5:9530/1‑146 (MQ=255)     aCGAGTCGCCATGCAGCACCACCTTGCCACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCagag                                                                                                                                               <  8:144040/149‑1 (MQ=255)        aGTCGCCATGCAGCACCACCTTGCCACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCg                                                                                                                                            <  6:31900/149‑1 (MQ=255)         gTCGCCATGCAGCACCACCTTGCCACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCg                                                                                                                                            <  2:349851/148‑1 (MQ=255)             ccATGCAGCACCACCTTGCCACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTcac                                                                                                                                       <  5:193736/149‑1 (MQ=255)                      accaccTTGCCACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCcgcg                                                                                                                              <  5:28093/149‑1 (MQ=255)                       ccaccTTGCCACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCgg                                                                                                                             >  5:217482/1‑149 (MQ=255)                                cACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGttct                                                                                                                    >  6:112441/1‑149 (MQ=255)                                cACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGACAGAGGCGGTcacc                                                                                                                                      >  3:65182/1‑131 (MQ=255)                                cACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGACAGAGGCGGTcacc                                                                                                                                      >  3:70460/1‑131 (MQ=255)                                cACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGACAGAGGCGGTcacc                                                                                                                                      <  4:65182/131‑1 (MQ=255)                                cACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGACAGAGGCGGTcacc                                                                                                                                      <  4:70460/131‑1 (MQ=255)                                 aCCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGttctt                                                                                                                   >  1:353015/1‑149 (MQ=255)                                           cgcACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAg                                                                                                         >  5:353324/1‑149 (MQ=255)                                           cgcACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAg                                                                                                         >  1:230984/1‑149 (MQ=255)                                              aCGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTg                                                                                                      >  6:88554/1‑149 (MQ=255)                                                gCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCATCACCCCGCgg                                                                                                                             >  5:202027/1‑124 (MQ=255)                                                gCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCATCACCCCGCgg                                                                                                                             <  6:202027/124‑1 (MQ=255)                                                       aCCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCtt                                                                                             >  2:48422/1‑149 (MQ=255)                                                        ccTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTg                                                                                            <  1:17562/149‑1 (MQ=255)                                                        ccTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTg                                                                                            <  6:210094/149‑1 (MQ=255)                                                         cTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTg                                                                                            >  4:142023/1‑148 (MQ=255)                                                         cTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTg                                                                                            <  3:142023/148‑1 (MQ=255)                                                         cTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGt                                                                                           <  5:369751/149‑1 (MQ=255)                                                         cTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGt                                                                                           >  5:187332/1‑149 (MQ=255)                                                         cTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGt                                                                                           >  8:190255/1‑149 (MQ=255)                                                                  cGGAGTGGTCTTGGGCGTCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACgcgc                                                                                  >  3:308809/1‑149 (MQ=255)                                                                         gTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGAt                                                                           <  5:88554/149‑1 (MQ=255)                                                                             tGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCtt                                                                        <  8:139698/148‑1 (MQ=255)                                                                              gggCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTg                                                                       >  4:25198/1‑148 (MQ=255)                                                                               ggCGTCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGaa                                                                     <  4:308809/149‑1 (MQ=255)                                                                               ggCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGaa                                                                     >  2:299472/1‑149 (MQ=255)                                                                                 cATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAAc                                                                    >  4:307806/1‑148 (MQ=255)                                                                                 cATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAAc                                                                    >  3:350685/1‑148 (MQ=255)                                                                                 cATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAAc                                                                    >  7:75103/1‑148 (MQ=255)                                                                                  aTCACGATGGTGTCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACga                                                                  <  4:45755/149‑1 (MQ=255)                                                                                   tCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACgag                                                                 <  6:187332/149‑1 (MQ=255)                                                                                     aCGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACgaga                                                                <  6:353324/148‑1 (MQ=255)                                                                                     aCGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACgaga                                                                <  2:230984/148‑1 (MQ=255)                                                                                                   gATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGt                                                 >  8:330594/1‑149 (MQ=255)                                                                                                       cccATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTc                                             <  3:25198/149‑1 (MQ=255)                                                                                                        ccATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTgg                                                         >  8:142448/1‑136 (MQ=255)                                                                                                        ccATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTgg                                                         <  7:142448/136‑1 (MQ=255)                                                                                                        ccATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTcg                                            <  1:299472/149‑1 (MQ=255)                                                                                                        ccATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTcg                                            >  6:144247/1‑149 (MQ=255)                                                                                                        ccATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTcg                                            >  2:220158/1‑149 (MQ=255)                                                                                                           tttCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCgcgc                                         >  5:290601/1‑149 (MQ=255)                                                                                                               aCGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGa                                     <  6:9530/149‑1 (MQ=255)                                                                                                               aCGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGa                                     <  8:75103/149‑1 (MQ=255)                                                                                                                             ccaggCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATAATGGCTGCAGGTCTTCGCGCTTGAGCAGAATATGGTTg                       >  6:180429/1‑149 (MQ=255)                                                                                                                               aggCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGcc                     >  1:97784/1‑149 (MQ=255)                                                                                                                                ggCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGccc                    <  7:190255/149‑1 (MQ=255)                                                                                                                                ggCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGccc                    <  8:239504/149‑1 (MQ=255)                                                                                                                                 gCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGcc                     >  2:194784/1‑147 (MQ=255)                                                                                                                                 gCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGcc                     >  3:303281/1‑147 (MQ=255)                                                                                                                                  ccTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGCCCAg                  <  6:217482/149‑1 (MQ=255)                                                                                                                                   cTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACACCGCGGGCCAGTACTTCCGGGCTCAGCTGGGCGACCtt                                                                                             >  1:29158/1‑73 (MQ=255)                                                                                                                                        gCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGCCCAGGCGCtt            >  3:111930/1‑149 (MQ=255)                                                                                                                                              ttGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGCCCAGGCGCTTGCTCAg      <  6:290601/149‑1 (MQ=255)                                                                                                                                                gCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGCCCAGGCGCTTGCTCAGCt    >  7:81698/1‑149 (MQ=255)                                                                                                                                                  cGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGCCCAGGCGCTTGCTCAGCTGc  <  4:350685/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GGGAACGAGTCGCCATGCAGCACCACCTTGCCACCGCGCGAGCGCACGCCTTCGACCTTGATCTCCGGAGTGGTCTTGGGCATCACGATGGTGGCCTTGATGCCCATTTCACGCGCGGCCAGGGCCAGGCCTTGGGCGTGGTTGCCGGCAGAGGCGGTCACCACCCCGCGGGCCAGTTCTTCCGGGCTCAGCTGGGCCAGCTTGTTATACGCGCCGCGGATCTTGAACGAGAATACTGGCTGCAGGTCTTCGCGCTTGAGCAGAATCTGGTTGCCCAGGCGCTTGCTCAGCTGC  >  NC_002947/5875797‑5876090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑