Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 464,320 G→C 8.2% H259Q (CAC→CAG)  davB ← lysine 2‑monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947464,3200GC 8.2% 142.7 / 4.3 49H259Q (CAC→CAG) davBlysine 2‑monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base C (2/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

GGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGA  >  NC_002947/464173‑464460
                                                                                                                                                   |                                                                                                                                            
ggcggcGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGt                                                                                                                                             <  5:55106/149‑1 (MQ=255)
            aaTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGgtcgt                                                                                                                                 >  8:109228/1‑149 (MQ=255)
             aTGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGgtcgtc                                                                                                                                <  6:56490/149‑1 (MQ=255)
              tGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCACGACCACCAGCACCTGGTGgtcgggg                                                                                                                               <  8:349129/149‑4 (MQ=255)
                cgcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGtcgtcg                                                                                                                               >  4:100499/1‑147 (MQ=255)
                 gcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAg                                                                                                                            <  7:21022/149‑1 (MQ=255)
                 gcTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTAAGTTGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGgtcgtggaag                                                                                                                            >  3:314611/1‑144 (MQ=255)
                     gACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTg                                                                                                                         <  5:291671/148‑1 (MQ=255)
                                gcgGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcgg                                                                                                                                 >  6:97727/1‑128 (MQ=255)
                                gcgGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGCACCTGGTGgtcgg                                                                                                                                 <  5:97727/129‑2 (MQ=255)
                                 cgGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCAcgc                                                                                                            <  4:37673/149‑1 (MQ=255)
                                  ggTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCAcgcg                                                                                                           >  2:193053/1‑149 (MQ=255)
                                         cgccATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGAtt                                                                                                    >  5:45860/1‑149 (MQ=255)
                                         ccgcATGCCCCGTGCTCAGGCGGCTGCCCTCTCTCCAATGCACGCACCGTTCCGGTACGTTTCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGAtt                                                                                                    <  4:314611/146‑1 (MQ=255)
                                             aTGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTc                                                                                                  >  3:275331/1‑147 (MQ=255)
                                                  gCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTACACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGAGGAAGATTTCCAGCATc                                                                                           >  2:167732/1‑149 (MQ=255)
                                                    gTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGa                                                                                                      <  5:390960/136‑1 (MQ=255)
                                                    gTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGa                                                                                                      >  6:390960/1‑136 (MQ=255)
                                                       cTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGtt                                                                                      <  2:167033/149‑1 (MQ=255)
                                                         cAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTACACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTACTCACGCCGCGGAAGATTTCCCGCTGAGAGTTgg                                                                                    >  4:279063/1‑149 (MQ=255)
                                                                  gCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCg                                                                           <  6:309581/149‑1 (MQ=255)
                                                                      tctGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                       >  7:16579/1‑149 (MQ=255)
                                                                        tGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTc                                                                       <  4:275331/147‑1 (MQ=255)
                                                                                 cACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAAccgccg                                                             <  1:167732/148‑1 (MQ=255)
                                                                                 cACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCgg                                                            <  6:371123/149‑1 (MQ=255)
                                                                                        cGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCCTCGCAGTTGGTCATCACCACGCGGAAGATTTCCCGCATCGAGTTGGGGAAGTACGCGTCCCACCCGCCGGTGCCGa                                                      >  3:253322/1‑148 (MQ=255)
                                                                                              ggTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGAc                                               >  1:132571/1‑149 (MQ=255)
                                                                                              ggTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGAc                                               <  1:193053/149‑1 (MQ=255)
                                                                                              ggTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACAGCCGGAGCAGAAACCGAc                                               >  6:156681/1‑149 (MQ=255)
                                                                                               gTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGAcc                                              >  5:342479/1‑149 (MQ=255)
                                                                                               gTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGAcc                                              <  8:16579/149‑1 (MQ=255)
                                                                                                      gcgcCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCg                                        >  4:345287/1‑148 (MQ=255)
                                                                                                       cgcCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGaa                                      <  5:156681/149‑1 (MQ=255)
                                                                                                         ccAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAaca                                    <  4:174744/149‑1 (MQ=255)
                                                                                                           aGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACt                                  >  7:356816/1‑149 (MQ=255)
                                                                                                             aTGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCACGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTGCAGCATCGAGTTGGGGAAGTCACGGTCCCAACCGCCGGTGCCGAAACCGACCGGGCCGACCACTTc                                >  5:238672/1‑149 (MQ=255)
                                                                                                                 cTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCgg                            >  5:349124/1‑149 (MQ=255)
                                                                                                                       gctcTTGTTCCACGCCCCCGACTACCAGGGGCTGGGGGTCGGCGCAGTTGGGCCTCACCCCGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCATCCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTgg                      <  4:253322/146‑1 (MQ=255)
                                                                                                                       gCACTTGTTCCACGCCCCCGCCCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTg                       >  7:78447/1‑148 (MQ=255)
                                                                                                                        cACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTgg                      >  7:266028/1‑148 (MQ=255)
                                                                                                                          cTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCTAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAg                   >  4:161664/1‑149 (MQ=255)
                                                                                                                             gTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCt                 >  5:291988/1‑148 (MQ=255)
                                                                                                                                ccACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGc             >  3:11102/1‑149 (MQ=255)
                                                                                                                                  aCGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCtt           >  5:4741/1‑149 (MQ=255)
                                                                                                                                       cccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCa       >  4:71134/1‑148 (MQ=255)
                                                                                                                                       cccGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCa       >  8:1202/1‑148 (MQ=255)
                                                                                                                                         cGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaaaa    <  7:109228/149‑1 (MQ=255)
                                                                                                                                         cGACCACCAGCACCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAAGCGACATGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCaaaa    >  6:291486/1‑149 (MQ=255)
                                                                                                                                           accaccAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAaga  <  5:297855/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
GGCGGCGCGGGCAATGCGCTTGACCCCGGTACGCGGTGCGCCGCCATGCAGCGTGCTCAGGCTGGTGCCCTCTGGCCAATGCACGCAGCGTTCCGGTACGTCGCGCCAGATGCCTTGTGGCACTTGTTCCACGCCCCCGACCACCAGGTGCTGGTGGTCGTCGCAGTTGGTCATCACCACGCGGAAGATTTCCAGCATCGAGTTGGGGAAGTCCGAGTCCCAACCGCCGGTGCCGAAACCGACCTGGCCGAACACTTCGCGGTGCTGGAAGCTCAGCTTGGCAAAAGA  >  NC_002947/464173‑464460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑