Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,986,841	T→A	100%	intergenic (+121/‑78)	PP_5245 → / → kefB‑III	AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,986,841	0	T	A	100.0%	224.9 / NA	66	intergenic (+121/‑78)	PP_5245/kefB‑III	AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (29/37);  total (29/37)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGC  >  NC_002947/5986703‑5986968                                                                                                                                            |                                                                                                                                 ggTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCACATGA‑TTGACTGACGCcac                                                                                                                        >  3:19331/1‑149 (MQ=255)      aCAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTAc                                                                                                                   >  8:259708/1‑149 (MQ=255)            cGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCa                                                                                                             <  5:55181/149‑1 (MQ=255)            cGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCACGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCt                                                                                                               >  1:60572/1‑147 (MQ=255)             gATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCt                                                                                                               >  4:236653/1‑146 (MQ=255)             gATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCt                                                                                                               >  7:57783/1‑146 (MQ=255)                 aGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGACTTGACTGACGCCACACTACCCCTCAGaa                                                                                                          <  1:22704/148‑1 (MQ=255)                   cgcTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGt                                                                                                      >  2:295431/1‑149 (MQ=255)                   cgcTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCACGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGt                                                                                                      <  2:60572/149‑1 (MQ=255)                    gcTTCCTTCTTCGCGGGCCCGCCCGCTCCGACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCTTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTc                                                                                                     <  4:273512/149‑1 (MQ=255)                     cTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGCTAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTcc                                                                                                    <  8:308727/149‑1 (MQ=255)                     cTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTcc                                                                                                    >  6:161218/1‑149 (MQ=255)                        ccTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACCCTACCCCTCAGAATTGTCCGt                                                                                                  >  5:19106/1‑148 (MQ=255)                        ccTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGAAGA‑TTGACTGACGCCACACTACACCTCAGAATTGACCGt                                                                                                  >  6:230648/1‑148 (MQ=255)                          ttcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCa                                                                                                >  2:143724/1‑148 (MQ=255)                          ttcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCa                                                                                                >  7:322083/1‑148 (MQ=255)                          ttcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCa                                                                                                >  4:103782/1‑148 (MQ=255)                                gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGt                                                                                          <  3:236653/148‑1 (MQ=255)                                 cgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGCTc                                                                                        <  2:122853/149‑1 (MQ=255)                                 cgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTAACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTc                                                                                        <  4:263879/149‑1 (MQ=255)                                      gcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAAc                                                                                    >  7:28332/1‑148 (MQ=255)                                      gcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACg                                                                                   <  7:5854/149‑1 (MQ=255)                                      gcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACg                                                                                   >  1:44595/1‑149 (MQ=255)                                        gcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTcc                                                                                                    <  5:378796/130‑1 (MQ=255)                                        gcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTcc                                                                                                    >  6:378796/1‑130 (MQ=255)                                          ccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGc                                                                               >  1:95689/1‑149 (MQ=255)                                                  acaGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACTCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAg                                                                       <  8:57783/149‑1 (MQ=255)                                                  acaGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAg                                                                       >  4:253247/1‑149 (MQ=255)                                                      agaTAGCACAGGGCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCa                                                                   <  6:19106/149‑1 (MQ=255)                                                       gaTAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCAt                                                                  <  5:146779/149‑1 (MQ=255)                                                        aTAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                 <  6:56629/149‑1 (MQ=255)                                                           gCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCGGGGCAGcgttcgaa                                                               <  2:95689/148‑8 (MQ=255)                                                           gCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa                                                               <  3:103782/148‑1 (MQ=255)                                                             acaGGCCTTGAGCACAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑Ta                                                           <  7:171433/149‑1 (MQ=255)                                                              caGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TAc                                                          >  2:142095/1‑149 (MQ=255)                                                                ggCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGa                                                        <  7:259708/149‑1 (MQ=255)                                                                ggCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGa                                                        <  1:143724/149‑1 (MQ=255)                                                                 gCCTTGAGCCCAGGTAAGCGACGGCACCGATTGTCCCTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACGACCCCTCAGAATTGTCGGGCAAACGGTTCGAACGGTGCCCCGGCAGCTCAGGGAG‑TACgcg                                                       >  4:62531/1‑147 (MQ=255)                                                                  ccTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGc                                                      <  5:148344/149‑1 (MQ=255)                                                                    ttGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAg                                                       >  5:219144/1‑146 (MQ=255)                                                                    ttGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAg                                                       <  6:219144/146‑1 (MQ=255)                                                                      gAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCa                                                    <  8:322083/147‑1 (MQ=255)                                                                       aGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAAt                                                  <  3:253247/148‑1 (MQ=255)                                                                                   cgcCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAgg                                      >  4:144754/1‑148 (MQ=255)                                                                                          aCCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTc                              >  4:111364/1‑149 (MQ=255)                                                                                           ccGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATTCCACACGAAGGCAGCCTTCt                             <  4:324480/149‑1 (MQ=255)                                                                                            cGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTg                            <  3:144754/149‑1 (MQ=255)                                                                                              aTTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGc                           >  6:40351/1‑148 (MQ=255)                                                                                                   cACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcg                     >  5:199613/1‑149 (MQ=255)                                                                                                    aCTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTcc                                                                                                    >  1:375337/1‑70 (MQ=255)                                                                                                    aCTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTcc                                                                                                    <  2:375337/70‑1 (MQ=255)                                                                                                    aCTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcgc                    <  2:44595/149‑1 (MQ=255)                                                                                                          gacaggacaATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGt                 <  8:28332/146‑1 (MQ=255)                                                                                                           acaggacaATTTTTCTTCCCGTCATATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATc             <  4:19331/149‑1 (MQ=255)                                                                                                           acaggacaATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGAt              <  5:40351/148‑1 (MQ=255)                                                                                                             aggacaATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCtt           >  8:261669/1‑149 (MQ=255)                                                                                                              ggaaaATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc          <  6:285754/149‑1 (MQ=255)                                                                                                               gacaATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGTCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTcc         >  7:120111/1‑149 (MQ=255)                                                                                                               gacaATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTcc         >  2:84806/1‑149 (MQ=255)                                                                                                                 caATTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGCTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCt        <  1:295431/149‑1 (MQ=255)                                                                                                                  aaTTTTTCTTCCCGTCAGATGT‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCGCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGc      <  5:230648/149‑1 (MQ=255)                                                                                                                   aTTTTTCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCt     <  5:161218/149‑1 (MQ=255)                                                                                                                    tttttCTTCCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCtt    <  7:261669/149‑1 (MQ=255)                                                                                                                       ttcttcCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTgc  <  6:16807/148‑1 (MQ=255)                                                                                                                          ttcCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGt                 >  7:4289/1‑130 (MQ=255)                                                                                                                          ttcCCGTCAGATGA‑TTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGt                 <  8:4289/130‑1 (MQ=255)                                                                                                                                            |                                                                                                                                 GGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGA‑TTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAG‑TACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGC  >  NC_002947/5986703‑5986968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑