Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,988,752 G→A 6.5% intergenic (+46/‑500) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,988,7520GA 6.5% 283.4 / 6.4 91intergenic (+46/‑500)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base G (39/46);  new base A (4/2);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCCAGGGGCAAGTGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA  >  NC_002947/5988607‑5988900
                                                                                                                                                 |                                                                                                                                                     
ggCCCAGGGGCAAGTGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGgcg                                                                                                                                                    >  7:196636/1‑149 (MQ=255)
   ccAGGGGCAAGTGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGcgccg                                                                                                                                                 >  5:185248/1‑149 (MQ=255)
   ccAGGGGCAAGGGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGGTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGcgccg                                                                                                                                                 <  8:349917/149‑1 (MQ=255)
      ggggCAAGTGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGccgccc                                                                                                                                              >  5:349308/1‑149 (MQ=255)
      ggggCAAGTGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGccgccc                                                                                                                                              >  6:297036/1‑149 (MQ=255)
       gggCAAGTGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGccgcccg                                                                                                                                             >  6:307189/1‑149 (MQ=255)
              tGCGTGATGACCGGGCCAAGGTGATGCAGCCGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCgaggcg                                                                                                                                      >  2:198034/1‑149 (MQ=255)
                      gaCCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCgg                                                                                                                              >  1:250793/1‑149 (MQ=255)
                       aCCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGGTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGGTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCgg                                                                                                                              <  5:37628/148‑1 (MQ=255)
                       aCCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGGGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACGGAGGCCTGTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCGCGGGCGgcgcccaatct                                                                                                                              <  3:217081/148‑8 (MQ=255)
                            gCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCgg                                                                                                                              >  2:113210/1‑143 (MQ=255)
                            gCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCgg                                                                                                                              <  1:113210/143‑1 (MQ=255)
                              cAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAAccc                                                                                                                      >  5:384389/1‑149 (MQ=255)
                                aGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGc                                                                                                                    >  2:200600/1‑149 (MQ=255)
                                 ggTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCt                                                                                                                   >  4:13345/1‑149 (MQ=255)
                                 ggTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCt                                                                                                                   >  3:141317/1‑149 (MQ=255)
                                 ggTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCGTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCt                                                                                                                   >  7:337856/1‑149 (MQ=255)
                                  gTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTc                                                                                                                  >  4:107896/1‑149 (MQ=255)
                                      tGCAGACGGCAAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTa                                                                                                               <  6:185248/148‑1 (MQ=255)
                                       gCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGc                                                                                                                    >  8:184631/1‑142 (MQ=255)
                                       gCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGc                                                                                                                    <  7:184631/142‑1 (MQ=255)
                                       gCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGAGGACTGAGGCCTATTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGCTAAACCCGAACCTACg                                                                                                             >  2:140887/1‑149 (MQ=39)
                                          gACTGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACgttt                                                                                                          <  4:141317/149‑1 (MQ=255)
                                          gACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACgttt                                                                                                          <  5:297036/149‑1 (MQ=255)
                                           aCGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCTCGGCGCTTCGCGGGTAAACCCGCTCCTACgtttg                                                                                                         <  5:54978/149‑1 (MQ=255)
                                               ccAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTc                                                                                                     <  2:226205/149‑1 (MQ=255)
                                               ccAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTc                                                                                                     >  3:249521/1‑149 (MQ=255)
                                                cAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCg                                                                                                    >  3:259674/1‑149 (MQ=255)
                                                  aggaggCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCggg                                                                                                  <  8:196636/149‑1 (MQ=255)
                                                  aggaggCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCggg                                                                                                  >  8:247767/1‑149 (MQ=255)
                                                      ggCGCGGGTGGAGCTGGAGAGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGTCGCGTGCCTTTAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGTGCCAg                                                                                              >  6:326282/1‑149 (MQ=255)
                                                      ggCGCGGGGGGCGCTGGAGCGGTTGGTTGCTTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAg                                                                                              <  8:337856/149‑1 (MQ=255)
                                                       gcgcGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGt                                                                                             <  8:270861/149‑1 (MQ=255)
                                                       gcgcGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGt                                                                                             <  2:269700/149‑1 (MQ=255)
                                                          cgGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTAt                                                                                          <  7:329477/149‑1 (MQ=255)
                                                              tggagctggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCGGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCt                                                                                      <  1:198034/149‑1 (MQ=255)
                                                                gagctggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCtgt                                                                                    <  1:200600/149‑1 (MQ=255)
                                                                gagctggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCtgt                                                                                    >  5:7793/1‑149 (MQ=255)
                                                                gagctggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCtgt                                                                                    <  4:249521/149‑1 (MQ=255)
                                                                  gctggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTcc                                                                                       <  3:222135/144‑1 (MQ=255)
                                                                  gctggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTcc                                                                                       >  4:222135/1‑144 (MQ=255)
                                                                     ggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGAc                                                                               >  3:20126/1‑149 (MQ=255)
                                                                     ggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGAc                                                                               >  2:182340/1‑149 (MQ=255)
                                                                      gagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCgg                                                                                                                              <  3:130751/101‑1 (MQ=255)
                                                                      gagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCgg                                                                                                                              >  4:130751/1‑101 (MQ=255)
                                                                      gagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGAc                                                                               <  6:349308/148‑1 (MQ=255)
                                                                        gcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCa                                                                                               >  8:791/1‑130 (MQ=255)
                                                                        gcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCa                                                                                               <  7:791/130‑1 (MQ=255)
                                                                        gcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTCTTTCGGGCCAGTGATTGCTGTGGGAcgcg                                                                            >  8:306234/1‑149 (MQ=255)
                                                                           gttgttTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CAc                                                                        >  2:124492/1‑149 (MQ=255)
                                                                                ttGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGa                                                                    <  5:138421/148‑1 (MQ=255)
                                                                                ttGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGa                                                                    <  2:148554/148‑1 (MQ=255)
                                                                                  agtggggATGCGGACTTAGGCCTTTTGGCGCGTGCCTTGAGGGGTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGc                                                                  <  1:140887/143‑1 (MQ=255)
                                                                                    ttCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCT‑CACGCGAGcccc                                                               >  8:279211/1‑149 (MQ=255)
                                                                                      cGGATGCGGTCTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCtgt                                                                                    <  4:10512/127‑1 (MQ=255)
                                                                                       ggATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTg                                                            <  4:183300/149‑1 (MQ=255)
                                                                                       ggATGCGGACTGAGGCCTTTTGGCGCGTGCCGTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCt                                                             <  5:307189/148‑1 (MQ=255)
                                                                                                   aGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTc                                                 <  3:107896/148‑1 (MQ=255)
                                                                                                    ggCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGa                                               <  7:306234/149‑1 (MQ=255)
                                                                                                     gCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGa                                               >  2:265520/1‑148 (MQ=255)
                                                                                                      ccTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTc                                                 <  8:101847/145‑1 (MQ=255)
                                                                                                      ccTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTc                                                 >  7:101847/1‑145 (MQ=255)
                                                                                                       cTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTc                                                                                        <  2:73870/106‑1 (MQ=255)
                                                                                                       cTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTc                                                                                        >  1:73870/1‑106 (MQ=255)
                                                                                                       cTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGatat                                            >  8:169329/1‑149 (MQ=255)
                                                                                                        ttttGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATc                                           >  7:296287/1‑149 (MQ=255)
                                                                                                              cgcgTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtcg                                     <  7:233326/149‑1 (MQ=255)
                                                                                                              cgcgTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCACGTATTCCTGTGGGACGCGTCACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtc                                      <  1:265520/149‑1 (MQ=255)
                                                                                                               gcgTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtcg                                     >  4:315587/1‑148 (MQ=255)
                                                                                                               gcgTGCCTTGAGGGTTGTAGTGCCTATGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtcg                                     >  6:40395/1‑148 (MQ=255)
                                                                                                                    ccTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTacaaa                               <  3:328234/149‑1 (MQ=255)
                                                                                                                     cTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTacaaac                              <  7:279211/149‑1 (MQ=255)
                                                                                                                         aGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAgg                          <  4:20126/149‑1 (MQ=255)
                                                                                                                          tggTTTTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGTGCCAGTTATTCCTGTGGGAGGCG‑GACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAgg                          <  5:326282/147‑1 (MQ=255)
                                                                                                                          gggTGGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGc                         <  4:157599/149‑1 (MQ=255)
                                                                                                                           ggTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGc                         <  3:13345/148‑1 (MQ=255)
                                                                                                                           ggTTGTAGTGCCTATGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCg                        <  5:40395/149‑1 (MQ=255)
                                                                                                                               tcagcgCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTG‑CGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTc                     <  7:174032/143‑1 (MQ=11)
                                                                                                                                 agcgCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTG‑CGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTcgcg                  >  7:228204/4‑149 (MQ=11)
                                                                                                                                  gcgCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTG‑CGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgc                 >  7:72951/3‑149 (MQ=11)
                                                                                                                                  gTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtcgt                                    <  8:247949/130‑1 (MQ=255)
                                                                                                                                  gTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtcgt                                    >  7:247949/1‑130 (MQ=255)
                                                                                                                                     ccTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTG‑CGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGGCGTACAAACAAGGCGGTcgcgcgc               >  5:339221/1‑148 (MQ=11)
                                                                                                                                       tgtgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTacaaaca                             <  2:251043/132‑1 (MQ=255)
                                                                                                                                       tgtgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTacaaaca                             >  1:251043/1‑132 (MQ=255)
                                                                                                                                         tgAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTg          >  2:159673/1‑149 (MQ=255)
                                                                                                                                          gagaTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTgg         <  6:7793/149‑1 (MQ=255)
                                                                                                                                          gagaTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGGCGCGCGCGCCTgg         <  1:182340/149‑1 (MQ=255)
                                                                                                                                          gagaTCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTG‑CGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTc                     >  3:369531/1‑137 (MQ=11)
                                                                                                                                          gagaTCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTG‑CGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTc                     <  4:369531/137‑1 (MQ=11)
                                                                                                                                              ttGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGcaca     <  2:182174/149‑1 (MQ=255)
                                                                                                                                                 ggCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACagga  <  1:159673/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GGCCCAGGGGCAAGTGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCG‑CACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA  >  NC_002947/5988607‑5988900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑