Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 465,442 A→G 7.1% L124L (TTG→CTG)  PP_0384 ← AsnC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947465,4420AG 7.1% 155.2 / 2.7 56L124L (TTG→CTG) PP_0384AsnC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (25/27);  new base G (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CCCGCTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACA  >  NC_002947/465299‑465590
                                                                                                                                               |                                                                                                                                                    
cccGCTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTgga                                                                                                                                                 >  8:306453/1‑147 (MQ=255)
 ccGCTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATc                                                                                                                                                >  1:308403/1‑149 (MQ=255)
    cTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAg                                                                                                                                             <  2:163874/149‑1 (MQ=255)
      ccTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAgct                                                                                                                                           >  2:281563/1‑149 (MQ=255)
        tACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAgctgc                                                                                                                                         >  6:335119/1‑149 (MQ=255)
         aCAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAgctgct                                                                                                                                        >  5:217943/1‑149 (MQ=255)
             ccGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCgac                                                                                                                                    <  2:271080/149‑3 (MQ=255)
             ccGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCggg                                                                                                                                    <  2:347761/149‑1 (MQ=255)
                gCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGc                                                                                                                                   >  5:332554/1‑147 (MQ=255)
                gCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCg                                                                                                                                  >  2:158204/1‑148 (MQ=255)
                gCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGa                                                                                                                                 <  5:38270/149‑1 (MQ=255)
                  cTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCgaccagat                                                                                                                               <  7:306453/149‑8 (MQ=255)
                        gATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCCCCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTAc                                                                                                                         >  7:150857/1‑149 (MQ=255)
                        gATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTAc                                                                                                                         <  8:25580/149‑1 (MQ=255)
                             ccccACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGc                                                                                                                    >  3:68558/1‑149 (MQ=255)
                              cccACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGc                                                                                                                    >  7:101548/1‑148 (MQ=255)
                                  cccGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGc                                                                                                               <  5:145165/149‑1 (MQ=255)
                                   ccGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGc                                                                                                               >  5:354858/1‑148 (MQ=255)
                                    cGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGa                                                                                                             >  2:98573/1‑149 (MQ=255)
                                     gAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGAc                                                                                                            <  1:158204/149‑1 (MQ=255)
                                           cAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTc                                                                                                      <  6:217943/149‑1 (MQ=255)
                                             gCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTAc                                                                                                                         <  1:325696/128‑1 (MQ=255)
                                             gCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTAc                                                                                                                         >  2:325696/1‑128 (MQ=255)
                                              cccGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTc                                                                                                      >  5:155613/1‑146 (MQ=255)
                                              cccGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTc                                                                                                      <  6:155613/146‑1 (MQ=255)
                                                                 cACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                                 >  3:107440/1‑148 (MQ=255)
                                                                     gTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACTTAGTCGAATTCGCCGCTTACCGCACACAgct                                                                            <  6:332554/149‑1 (MQ=255)
                                                                        cACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTAGGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAgctgc                                                                          <  1:281563/148‑1 (MQ=255)
                                                                              gctgctGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCt                                                                   >  4:45880/1‑149 (MQ=255)
                                                                                  ctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGTTCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGcc                                                               >  1:329960/1‑149 (MQ=255)
                                                                                  ctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGAc                                                                                                            >  8:119710/1‑104 (MQ=255)
                                                                                  ctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGAc                                                                                                            <  7:119710/104‑1 (MQ=255)
                                                                                  ctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTgg                                                                 <  6:354858/147‑1 (MQ=255)
                                                                                     agaATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGccc                                                              <  6:183487/147‑1 (MQ=255)
                                                                                      gaATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATg                                                           <  4:68558/149‑1 (MQ=255)
                                                                                      gaATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATg                                                           <  4:107440/149‑1 (MQ=255)
                                                                                            tCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGTTCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTc                                                     <  2:329960/149‑1 (MQ=255)
                                                                                              gACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCa                                                    <  6:216989/148‑1 (MQ=255)
                                                                                                           tctcCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGcaccac                                       <  8:14451/148‑1 (MQ=255)
                                                                                                           tctcCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGcaccac                                       <  3:241019/148‑1 (MQ=255)
                                                                                                                 ccccGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTg                                >  8:15497/1‑149 (MQ=255)
                                                                                                                  cccGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGc                               >  4:56274/1‑149 (MQ=255)
                                                                                                                   ccGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTg                                >  7:166933/1‑147 (MQ=255)
                                                                                                                   ccGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGcc                              <  5:335119/149‑1 (MQ=255)
                                                                                                                   ccGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGcc                              >  1:92195/1‑149 (MQ=255)
                                                                                                                   ccGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAAATCGCCGCTTACCGCACACAGCTGCTGCACCTCGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGcc                              >  7:51990/1‑149 (MQ=255)
                                                                                                                    cGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCg                             <  8:101548/149‑1 (MQ=255)
                                                                                                                         acacTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCAc                                                                     >  1:100882/1‑104 (MQ=255)
                                                                                                                         acacTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCAc                                                                     <  2:100882/104‑1 (MQ=255)
                                                                                                                            cTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTgg                     <  2:308403/149‑1 (MQ=255)
                                                                                                                               ccAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCGCACAGCTGCTGCACCTGGCCCATGGCACTc                                                     >  5:114630/1‑114 (MQ=255)
                                                                                                                               ccAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCGCACAGCTGCTGCACCTGGCCCATGGCACTc                                                     <  6:114630/114‑1 (MQ=255)
                                                                                                                                       gTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTg           <  2:381013/148‑1 (MQ=255)
                                                                                                                                       gTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTg           <  3:56274/148‑1 (MQ=255)
                                                                                                                                        tCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACCGCTGCTGCACCTGGCCGATGGCCCTCAGGCGCCGCCCCACGTCCTTGCCGGAGTGTGGCGGCACCTTGAt         >  6:368393/1‑149 (MQ=255)
                                                                                                                                        ccGAGCAGCTGGATCAGTTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGAt         >  2:301703/2‑149 (MQ=255)
                                                                                                                                              aaCTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGAc   <  2:92195/149‑1 (MQ=255)
                                                                                                                                               aCTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACa  >  1:277823/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCCGCTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGGCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACA  >  NC_002947/465299‑465590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑