Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,995,016 G→A 8.2% intergenic (+144/+33) PP_5251 → / ← PP_5252 transporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,995,0160GA 8.2% 174.2 / 4.5 61intergenic (+144/+33)PP_5251/PP_5252transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/29);  new base A (3/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.73e-01

ATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACAAGGCCCG  >  NC_002947/5994868‑5995163
                                                                                                                                                     |                                                                                                                                                   
aTTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGAGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAg                                                                                                                                                     >  6:229929/1‑149 (MQ=255)
     tGCCGGCCCTTACGGCCCATTCGGGGGTGAACCCGCTCCTACACCTATCTGCGGTAGACACACACCGGAGGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTAt                                                                                                                                                 <  2:382295/148‑1 (MQ=255)
       ccGGCCCTTACGGCCCATTCGCGAGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTgg                                                                                                                                              <  6:39757/149‑1 (MQ=255)
        cGGCCCTTACGGCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGc                                                                                                                                             <  6:387842/149‑1 (MQ=255)
         ggCCCTTACGGCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGcc                                                                                                                                            <  7:328049/149‑1 (MQ=255)
                   gCCCATTCGCGGTTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTc                                                                                                                                  <  1:47488/149‑1 (MQ=255)
                   gCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAATGAGGCCGTTGTAGGAGCGGGTT‑TAGCCGCGAAAGGGCCGGCACTGAAGACCCAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTc                                                                                                                                  <  8:25892/149‑1 (MQ=255)
                    cccATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTca                                                                                                                                 <  3:79236/149‑1 (MQ=255)
                      cATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGGTCGTccca                                                                                                                               >  4:296416/1‑149 (MQ=255)
                            cgGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACAtcagct                                                                                                                         <  1:237114/149‑1 (MQ=255)
                             gggTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAATAACTGGccc                                                                                                                                           <  6:16914/130‑1 (MQ=255)
                             gggTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAATAACTGGccc                                                                                                                                           >  5:16914/1‑130 (MQ=255)
                                  aaCCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGACACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCtt                                                                                                                   <  6:99276/149‑1 (MQ=255)
                                     ccGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGa                                                                                                                <  8:186396/149‑1 (MQ=255)
                                        cTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAg                                                                                                             <  2:168456/149‑1 (MQ=255)
                                              cacaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTATACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCATATCAGCTCAGCTTAGAAAGCGagg                                                                                                        >  1:305352/1‑147 (MQ=255)
                                              cacaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAg                                                                                                        <  5:229929/148‑1 (MQ=255)
                                              cacaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGc                                                                                                       <  4:239511/149‑1 (MQ=255)
                                               acaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGACACATAAGCTCAGCTTACAAAGCGAAgaa                                                                                                      >  8:29975/1‑147 (MQ=255)
                                                 aGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATCGGcc                                                                                                                                            >  2:28143/1‑109 (MQ=255)
                                                 aGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATCGGcc                                                                                                                                            <  1:28143/109‑1 (MQ=255)
                                                           tAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAg                                                                                          >  2:330233/1‑149 (MQ=255)
                                                             gACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAgcg                                                                                        >  7:28608/1‑149 (MQ=255)
                                                             gACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAgcg                                                                                        >  5:256748/1‑149 (MQ=255)
                                                                     ccGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGACAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCAccc                                                                                   >  2:314260/1‑146 (MQ=255)
                                                                      cGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAg                                                                                >  4:128088/1‑148 (MQ=255)
                                                                       ggACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGaa                                                                              <  8:18068/149‑1 (MQ=255)
                                                                        gACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAg                                                                             >  7:220831/1‑149 (MQ=255)
                                                                             tGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGcc                                                                                             <  6:112152/128‑1 (MQ=255)
                                                                             tGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGcc                                                                                             >  5:112152/1‑128 (MQ=255)
                                                                                aaaCAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGCAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTgg                                                                       >  2:325275/1‑147 (MQ=255)
                                                                                aaaCAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGaa                                                                     <  7:342514/149‑1 (MQ=255)
                                                                                    aGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCa                                                                 <  3:128088/149‑1 (MQ=255)
                                                                                      gCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCAtt                                                               >  3:258928/1‑149 (MQ=255)
                                                                                         gTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTAc                                                            >  3:270201/1‑149 (MQ=255)
                                                                                         gTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTAc                                                            <  4:178164/149‑1 (MQ=255)
                                                                                                gAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACAtc                                                                                                                             <  8:111494/77‑1 (MQ=255)
                                                                                                gAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACAtc                                                                                                                             >  7:111494/1‑77 (MQ=255)
                                                                                                       tt‑tACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAAt                                              <  8:28608/149‑1 (MQ=255)
                                                                                                             ccGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCcgc                                         <  4:270201/149‑1 (MQ=255)
                                                                                                              cgcgAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCcgcg                                        <  1:314260/149‑1 (MQ=255)
                                                                                                                cgAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGcc                                      >  2:373079/1‑149 (MQ=255)
                                                                                                                cgAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGcc                                      >  2:378822/1‑149 (MQ=255)
                                                                                                                  aaaGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCtt                                    >  2:256700/1‑149 (MQ=255)
                                                                                                                         cGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTgg                             >  7:152927/1‑149 (MQ=255)
                                                                                                                               tGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTgc                       >  1:283689/1‑149 (MQ=255)
                                                                                                                                      acaAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGATGAGCGCATGca                >  4:221176/1‑149 (MQ=255)
                                                                                                                                        aaGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGcaca              >  3:292964/1‑149 (MQ=255)
                                                                                                                                         gaacgggggCCAATAACTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTgc                       >  6:19469/6‑139 (MQ=255)
                                                                                                                                         gaacgggggCCAATAACTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTgc                       <  5:19469/134‑1 (MQ=255)
                                                                                                                                         gaacgggggCCAATAACTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac             >  4:81607/6‑149 (MQ=255)
                                                                                                                                         aGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac             >  1:170263/1‑149 (MQ=255)
                                                                                                                                          gAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCAcacc            <  2:170263/149‑1 (MQ=255)
                                                                                                                                            aaGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCAcaccac          <  4:292964/149‑1 (MQ=255)
                                                                                                                                             aGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCAcaccac          >  7:192196/1‑148 (MQ=255)
                                                                                                                                              ggggCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACaa        <  1:256700/149‑1 (MQ=255)
                                                                                                                                               gggCCAGTTATTGGGCGCAGTTCGTGACATCAGCTCAGCTTAGAAAGCGAAGCACGAAGAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTAGCGGCACGCTAGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCTCACCACAAg       <  7:29975/149‑1 (MQ=255)
                                                                                                                                                 gCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac             >  7:269993/1‑141 (MQ=255)
                                                                                                                                                 gCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac             <  8:269993/141‑1 (MQ=255)
                                                                                                                                                   cAGTTATTGTCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACACCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTGTTCGGGGCAGTGCGCCTTcac               >  6:224772/1‑137 (MQ=255)
                                                                                                                                                   cAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGcac               <  5:224772/137‑1 (MQ=255)
                                                                                                                                                    aGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACAAGGCCCg  >  8:188166/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACAAGGCCCG  >  NC_002947/5994868‑5995163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑