Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,995,018	T→A	8.2%	intergenic (+146/+31)	PP_5251 → / ← PP_5252	transporter/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,995,018	0	T	A	8.2%	168.2 / 5.7	61	intergenic (+146/+31)	PP_5251/PP_5252	transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/29);  new base A (3/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01

TGCCGGCCCTTACGGCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACAAGGCCCG  >  NC_002947/5994873‑5995163                                                                                                                                                   |                                                                                                                                                  tGCCGGCCCTTACGGCCCATTCGGGGGTGAACCCGCTCCTACACCTATCTGCGGTAGACACACACCGGAGGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTAt                                                                                                                                                 <  2:382295/148‑1 (MQ=255)   ccGGCCCTTACGGCCCATTCGCGAGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTgg                                                                                                                                              <  6:39757/149‑1 (MQ=255)    cGGCCCTTACGGCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGc                                                                                                                                             <  6:387842/149‑1 (MQ=255)     ggCCCTTACGGCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGcc                                                                                                                                            <  7:328049/149‑1 (MQ=255)               gCCCATTCGCGGTTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTc                                                                                                                                  <  1:47488/149‑1 (MQ=255)               gCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAATGAGGCCGTTGTAGGAGCGGGTT‑TAGCCGCGAAAGGGCCGGCACTGAAGACCCAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTc                                                                                                                                  <  8:25892/149‑1 (MQ=255)                cccATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTca                                                                                                                                 <  3:79236/149‑1 (MQ=255)                  cATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGGTCGTccca                                                                                                                               >  4:296416/1‑149 (MQ=255)                        cgGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACAtcagct                                                                                                                         <  1:237114/149‑1 (MQ=255)                         gggTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAATAACTGGccc                                                                                                                                           <  6:16914/130‑1 (MQ=255)                         gggTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAATAACTGGccc                                                                                                                                           >  5:16914/1‑130 (MQ=255)                              aaCCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGACACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCtt                                                                                                                   <  6:99276/149‑1 (MQ=255)                                 ccGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGa                                                                                                                <  8:186396/149‑1 (MQ=255)                                    cTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAg                                                                                                             <  2:168456/149‑1 (MQ=255)                                          cacaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTATACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCATATCAGCTCAGCTTAGAAAGCGagg                                                                                                        >  1:305352/1‑147 (MQ=255)                                          cacaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAg                                                                                                        <  5:229929/148‑1 (MQ=255)                                          cacaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGc                                                                                                       <  4:239511/149‑1 (MQ=255)                                           acaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGACACATAAGCTCAGCTTACAAAGCGAAgaa                                                                                                      >  8:29975/1‑147 (MQ=255)                                             aGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATCGGcc                                                                                                                                            <  1:28143/109‑1 (MQ=255)                                             aGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATCGGcc                                                                                                                                            >  2:28143/1‑109 (MQ=255)                                                       tAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAg                                                                                          >  2:330233/1‑149 (MQ=255)                                                         gACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAgcg                                                                                        >  7:28608/1‑149 (MQ=255)                                                         gACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAgcg                                                                                        >  5:256748/1‑149 (MQ=255)                                                                 ccGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGACAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCAccc                                                                                   >  2:314260/1‑146 (MQ=255)                                                                  cGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAg                                                                                >  4:128088/1‑148 (MQ=255)                                                                   ggACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGaa                                                                              <  8:18068/149‑1 (MQ=255)                                                                    gACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAg                                                                             >  7:220831/1‑149 (MQ=255)                                                                         tGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGcc                                                                                             <  6:112152/128‑1 (MQ=255)                                                                         tGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGcc                                                                                             >  5:112152/1‑128 (MQ=255)                                                                            aaaCAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGCAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTgg                                                                       >  2:325275/1‑147 (MQ=255)                                                                            aaaCAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGaa                                                                     <  7:342514/149‑1 (MQ=255)                                                                                aGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCa                                                                 <  3:128088/149‑1 (MQ=255)                                                                                  gCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCAtt                                                               >  3:258928/1‑149 (MQ=255)                                                                                     gTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTAc                                                            <  4:178164/149‑1 (MQ=255)                                                                                     gTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTAc                                                            >  3:270201/1‑149 (MQ=255)                                                                                            gAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACAtc                                                                                                                             <  8:111494/77‑1 (MQ=255)                                                                                            gAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACAtc                                                                                                                             >  7:111494/1‑77 (MQ=255)                                                                                                   tt‑tACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAAt                                              <  8:28608/149‑1 (MQ=255)                                                                                                         ccGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCcgc                                         <  4:270201/149‑1 (MQ=255)                                                                                                          cgcgAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCcgcg                                        <  1:314260/149‑1 (MQ=255)                                                                                                            cgAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGcc                                      >  2:373079/1‑149 (MQ=255)                                                                                                            cgAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGcc                                      >  2:378822/1‑149 (MQ=255)                                                                                                              aaaGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCtt                                    >  2:256700/1‑149 (MQ=255)                                                                                                                     cGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTgg                             >  7:152927/1‑149 (MQ=255)                                                                                                                           tGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTgc                       >  1:283689/1‑149 (MQ=255)                                                                                                                                  acaAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGATGAGCGCATGca                >  4:221176/1‑149 (MQ=255)                                                                                                                                    aaGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGcaca              >  3:292964/1‑149 (MQ=255)                                                                                                                                     gaacgggggCCAATAACTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTgc                       >  6:19469/6‑139 (MQ=255)                                                                                                                                     gaacgggggCCAATAACTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTgc                       <  5:19469/134‑1 (MQ=255)                                                                                                                                     gaacgggggCCAATAACTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac             >  4:81607/6‑149 (MQ=255)                                                                                                                                     aGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac             >  1:170263/1‑149 (MQ=255)                                                                                                                                      gAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCAcacc            <  2:170263/149‑1 (MQ=255)                                                                                                                                        aaGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCAcaccac          <  4:292964/149‑1 (MQ=255)                                                                                                                                         aGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCAcaccac          >  7:192196/1‑148 (MQ=255)                                                                                                                                          ggggCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACaa        <  1:256700/149‑1 (MQ=255)                                                                                                                                           gggCCAGTTATTGGGCGCAGTTCGTGACATCAGCTCAGCTTAGAAAGCGAAGCACGAAGAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTAGCGGCACGCTAGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCTCACCACAAg       <  7:29975/149‑1 (MQ=255)                                                                                                                                             gCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac             >  7:269993/1‑141 (MQ=255)                                                                                                                                             gCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac             <  8:269993/141‑1 (MQ=255)                                                                                                                                               cAGTTATTGTCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACACCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTGTTCGGGGCAGTGCGCCTTcac               >  6:224772/1‑137 (MQ=255)                                                                                                                                               cAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGcac               <  5:224772/137‑1 (MQ=255)                                                                                                                                                aGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACAAGGCCCg  >  8:188166/1‑149 (MQ=255)                                                                                                                                                  ttATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac             >  6:143161/1‑136 (MQ=255)                                                                                                                                                  ttATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac             <  5:143161/136‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TGCCGGCCCTTACGGCCCATTCGCGGGTGAACCCGCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTT‑TACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACAAGGCCCG  >  NC_002947/5994873‑5995163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑