Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 467,101 C→T 11.2% intergenic (‑34/+19) PP_5445 ← / ← PP_t71 hypothetical protein/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947467,1010CT11.2% 214.2 / 13.4 80intergenic (‑34/+19)PP_5445/PP_t71hypothetical protein/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base C (34/37);  new base T (4/5);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

CTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATCTGA  >  NC_002947/466957‑467245
                                                                                                                                                |                                                                                                                                                
cTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATcgcgc                                                                                                                                              <  2:288160/149‑1 (MQ=255)
 tGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGTATGAAAAAGCCGCGCAATcgcgcg                                                                                                                                             >  8:8054/1‑149 (MQ=255)
 tGGGTGACCGGCTTCTTGGGGGGGCTTATTTCGCTGCGCTACAGTTTTCGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTTCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATcgcgcg                                                                                                                                             <  7:75507/149‑1 (MQ=255)
 tGGGGGACCGGCTTCTTGGGGGGGCTGATGTCGCTGAGAGACCGTGTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCGGGAGGTACGCGCCCCTGCCCATTTATTTCTTGCGGGCATGAAACAGCCGCGCAATcgcgcg                                                                                                                                             <  7:70324/149‑1 (MQ=255)
    gTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCgctgct                                                                                                                                          <  8:184425/149‑1 (MQ=255)
       aCCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCttt                                                                                                                                        <  6:144938/148‑1 (MQ=255)
        ccGGCTTCTTGGTGGGGCTGATTGCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGt                                                                                                                                      <  7:159368/149‑1 (MQ=255)
         cGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGCTTTTTc                                                                                                                                     <  1:152646/149‑1 (MQ=255)
         cGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGCTTTTTc                                                                                                                                     <  1:170866/149‑1 (MQ=255)
         cGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGCTTTTTc                                                                                                                                     <  3:214246/149‑1 (MQ=255)
                   gtggGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGTATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtggt                                                                                                                            <  7:8054/148‑1 (MQ=255)
                   gtggGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtggtg                                                                                                                           <  3:299339/149‑1 (MQ=255)
                      gggCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTCCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGc                                                                                                                        >  5:362593/1‑149 (MQ=255)
                        gCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGcc                                                                                                                       >  5:48178/1‑148 (MQ=255)
                        gCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGcc                                                                                                                       >  4:232866/1‑148 (MQ=255)
                        gCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGcc                                                                                                                       >  1:69186/1‑148 (MQ=255)
                            aTTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCacac                                                                                                                  >  6:101264/1‑149 (MQ=255)
                             tttCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACg                                                                                                                 <  2:263919/149‑1 (MQ=255)
                                  cTGAGTTAGCGTTTTAGCGCTACTTCCAAGGTCCTGGGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTTACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGGGGCTTTGTCGTATCTGGTGGGCCCACACGGACTc                                                                                                            <  6:362593/149‑1 (MQ=255)
                                     agaTCCCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAACCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGaa                                                                                                         <  2:327914/149‑1 (MQ=255)
                                        tACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCg                                                                                                      >  4:316446/1‑149 (MQ=255)
                                         aCCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGt                                                                                                     >  6:31311/1‑149 (MQ=255)
                                         aCCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGt                                                                                                     >  2:351292/1‑149 (MQ=255)
                                           cGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTgg                                                                                                   <  4:344621/149‑1 (MQ=255)
                                            gTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGa                                                                                                  <  1:350604/149‑1 (MQ=255)
                                            gTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGa                                                                                                  <  3:232866/149‑1 (MQ=255)
                                               ttAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACGATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGagc                                                                                                >  7:356408/1‑146 (MQ=255)
                                                    gcTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGa                                                                                          <  6:276737/149‑1 (MQ=255)
                                                     cTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCa                                                                                               <  6:104884/143‑1 (MQ=255)
                                                     cTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCa                                                                                               >  5:104884/1‑143 (MQ=255)
                                                        cTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCGCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGa                                                                                                               >  1:273597/1‑124 (MQ=255)
                                                        cTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGa                                                                                                               <  2:273597/124‑1 (MQ=255)
                                                        cTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTAt                                                                                      <  6:48178/149‑1 (MQ=255)
                                                          tCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTAt                                                                                      >  1:318886/1‑147 (MQ=255)
                                                           ccAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGa                                                                                    <  1:81939/148‑1 (MQ=255)
                                                           ccAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAg                                                                                   >  3:221419/1‑149 (MQ=255)
                                                           ccAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAg                                                                                   >  3:280817/1‑149 (MQ=255)
                                                            cAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTAt                                                                                      >  8:183932/1‑145 (MQ=255)
                                                            cAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTAt                                                                                      <  7:183932/145‑1 (MQ=255)
                                                             aaGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTc                                                                                 >  8:330347/1‑149 (MQ=255)
                                                                   aTGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGc                                                                           >  4:289728/1‑149 (MQ=255)
                                                                    tGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGct                                                                          <  2:359531/149‑1 (MQ=255)
                                                                    tGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGct                                                                          >  1:359531/1‑149 (MQ=255)
                                                                     gcgcTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGctc                                                                         >  7:17953/1‑149 (MQ=255)
                                                                         tCGGCGCCGCGGCGCTGGATGTACGCGCCCCTTACCATTTAATTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGGGGCTTTGTCGTATCTGGTGGGCCCACACGGAGTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTacc                                                                     <  3:25360/149‑3 (MQ=255)
                                                                           ggCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCTATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAAc                                                                     <  2:318886/147‑1 (MQ=255)
                                                                           ggCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAAc                                                                     <  3:316446/147‑1 (MQ=255)
                                                                            gcgcCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCaa                                                                  <  7:330347/149‑1 (MQ=255)
                                                                                cgctgcgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGa                                                              <  1:103391/149‑1 (MQ=255)
                                                                                 gctgcgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAg                                                             <  4:261912/149‑1 (MQ=255)
                                                                                 gctgcgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAg                                                             <  5:31311/149‑1 (MQ=255)
                                                                                  ctgcgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGc                                                            >  2:13104/1‑149 (MQ=255)
                                                                                     cgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCtat                                                         <  5:101264/149‑1 (MQ=255)
                                                                                       ctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAg                                                       <  3:289728/149‑1 (MQ=255)
                                                                                          gATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGc                                                     <  6:186942/148‑1 (MQ=255)
                                                                                          gATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGc                                                     >  5:186942/1‑148 (MQ=255)
                                                                                             gTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCt                                                  >  6:284525/1‑148 (MQ=255)
                                                                                               aCGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAg                                               >  6:350466/1‑149 (MQ=255)
                                                                                                    ccccTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGt                                          <  8:17953/149‑1 (MQ=255)
                                                                                                          aCCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCg                                     <  4:280817/148‑1 (MQ=255)
                                                                                                            cATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGAt                                  >  1:253905/1‑149 (MQ=255)
                                                                                                             atttatttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGcc                                                    >  2:213062/1‑130 (MQ=255)
                                                                                                             atttatttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGcc                                                    <  1:213062/130‑1 (MQ=255)
                                                                                                              tttatttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATta                                <  5:350466/149‑1 (MQ=255)
                                                                                                              tttatttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATta                                >  7:324469/1‑149 (MQ=255)
                                                                                                              tttatttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATta                                >  1:184996/1‑149 (MQ=255)
                                                                                                                           ggCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTc                   <  1:42828/149‑1 (MQ=255)
                                                                                                                             cATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCg                  >  5:18434/1‑148 (MQ=255)
                                                                                                                                gAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATtat                               <  6:47737/132‑1 (MQ=255)
                                                                                                                                gAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATtat                               >  5:47737/1‑132 (MQ=255)
                                                                                                                                gAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGttt                    >  7:279628/1‑143 (MQ=255)
                                                                                                                                gAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGttt                    <  8:279628/143‑1 (MQ=255)
                                                                                                                                 aaaaaGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGACCCGTGGACCAAAGGATTATGAGTCCTCTGCTCTCACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCt             >  7:39113/1‑149 (MQ=255)
                                                                                                                                  caaaGCCGCGCGATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGTGTTTGGCTCCtg            >  4:250223/2‑149 (MQ=39)
                                                                                                                                  aaaaGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtg            >  7:366325/1‑149 (MQ=255)
                                                                                                                                   aaaGCCGCGCGATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTACCCACCTGAGCTAAAGGCCGTCAGTAAGTGGGCGGATTTTAACGAGGGGTTCGCACCtgt           >  7:328660/1‑149 (MQ=255)
                                                                                                                                   aaaGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtgt           <  1:351292/149‑1 (MQ=255)
                                                                                                                                    aaGCCGCGCGATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtgtg          >  4:333515/1‑149 (MQ=255)
                                                                                                                                    aaGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtgtg          <  8:324469/149‑1 (MQ=255)
                                                                                                                                     aGCCGCGCGATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGc         >  7:280214/1‑149 (MQ=255)
                                                                                                                                          cgcgATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATCt    <  3:333515/149‑1 (MQ=255)
                                                                                                                                             aaTCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCGATAGGGCCTCAGGAAGTGGGCGGATTATAACGAGGGGTTCGCACCTGGGCCATCTGa  >  8:82826/1‑148 (MQ=255)
                                                                                                                                               tCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGc                                                     >  6:42285/1‑95 (MQ=255)
                                                                                                                                               tCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGc                                                     <  5:42285/95‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATCTGA  >  NC_002947/466957‑467245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑