Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,030,354	T→G	5.1%	intergenic (+39/+28)	PP_5729 → / ← rpmG	hypothetical protein/50S ribosomal protein L33

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,030,354	0	T	G	5.1%	235.7 / 3.7	78	intergenic (+39/+28)	PP_5729/rpmG	hypothetical protein/50S ribosomal protein L33
Reads supporting (aligned to +/- strand):  ref base T (36/37);  new base G (2/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CCGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTCGGTGGTGTAGAAGTGGCCG  >  NC_002947/6030210‑6030499                                                                                                                                                 |                                                                                                                                                    ccGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCACCAGCCAAAAAAAACCCCGCCTCCGTTCCGATCCgg                                                                                                                                                 >  8:278515/1‑149 (MQ=255) ccGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCgg                                                                                                                                                 <  2:86130/149‑1 (MQ=255)    ggtggtCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑tt                                                                                                                                             <  4:118117/149‑1 (MQ=255)         tCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑ttttttt                                                                                                                                        >  1:74613/1‑149 (MQ=255)         tCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑tttttt                                                                                                                                         >  8:226294/1‑148 (MQ=255)                 aaCGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGc                                                                                                                                >  8:96399/1‑149 (MQ=255)                 aaCGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTATTTTCAGGTCGc                                                                                                                                >  7:71234/1‑149 (MQ=255)                   cGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGcc                                                                                                                               >  2:63840/1‑148 (MQ=255)                    gAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTCCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGAAGCGGG‑TTTTTTTCAGGTCGCCg                                                                                                                              >  2:202576/1‑148 (MQ=255)                     aaaTGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGa                                                                                                                             >  6:339311/1‑148 (MQ=255)                      aaTGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGAt                                                                                                                            >  8:295747/1‑148 (MQ=255)                          tgCACCCGCGAGTACGACGTCGAGCGCATCGAGATCATGGCTGGTGCGGACCTGCTGATCCAGTGCTTCGACTTCAACTGACCCTGGAACAGCCAAAAAAAAACCGGCCTGCTTTCGTATGCGGG‑TTTTTTTCAGGTCGCCGATCAAt                                                                                                                        <  8:49583/148‑1 (MQ=255)                               ccGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACtt                                                                                                                   >  4:21984/1‑148 (MQ=255)                                      tACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTgg                                                                                                           <  3:72331/149‑1 (MQ=255)                                        cgacgTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGc                                                                                                          <  2:74613/148‑1 (MQ=255)                                         gacgTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCtt                                                                                                        >  2:68821/1‑149 (MQ=255)                                         gacgTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTATTTCAGGTCGCCGATCAATTACTTGATCTTGGCtt                                                                                                        >  3:367507/1‑149 (MQ=255)                                          acgTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTc                                                                                                       <  7:295747/149‑1 (MQ=255)                                           cgTCGAGCGGATCCAGATCAAGGCCGGTGGCGACCTGCTGATCCATGGGTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGGCGATCAATTACTTGATGTTGGCTTcc                                                                                                      <  4:367507/149‑1 (MQ=255)                                           cgTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTcc                                                                                                      >  7:259396/1‑149 (MQ=255)                                             tCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCtt                                                                                                    <  1:68821/149‑1 (MQ=255)                                               gAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTTACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGt                                                                                                  <  7:226294/149‑1 (MQ=255)                                               gAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTg                                                                                                   >  8:108508/1‑148 (MQ=255)                                               gAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGt                                                                                                  <  5:93682/149‑1 (MQ=255)                                                  cgcATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTg                                                                                                            >  2:390310/1‑136 (MQ=255)                                                  cgcATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTg                                                                                                            <  1:390310/136‑1 (MQ=255)                                                    cATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTAcacc                                                                                             <  3:21984/149‑1 (MQ=255)                                                       ccAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGa                                                                                                                 <  3:214380/126‑1 (MQ=255)                                                       ccAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGa                                                                                                                 >  4:214380/1‑126 (MQ=255)                                                              atggCCGGTTCCGACCTGCTGATCAAGGGATTCGACTTCAACTTCACCTGCAACAGACAAAAAAACACCCGCCTCCTTTCGCATGCGTG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGc                                                                                   <  8:13326/147‑1 (MQ=255)                                                              aaGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTcc                                                                                                      <  3:246147/130‑1 (MQ=255)                                                              aaGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTcc                                                                                                      >  4:246147/1‑130 (MQ=255)                                                               aGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGc                                                                                   >  7:144284/1‑148 (MQ=255)                                                               aGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCg                                                                                  <  5:20816/149‑1 (MQ=255)                                                                 gCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGaa                                                                                >  4:318472/1‑149 (MQ=255)                                                                 gCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCATCCGAAAGGAGGCGGG‑tttttt                                                                                                                                         <  8:19680/92‑1 (MQ=25)                                                                           aCCTGCTTATCCAGGGCTTCGAATTCAACTTACCCTTCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGa                                                                                 <  6:307956/138‑1 (MQ=255)                                                                           aCCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGATTGCGa                                                                                 >  5:307956/1‑138 (MQ=255)                                                                             ctgctgATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCat                                                                    <  7:108508/149‑1 (MQ=255)                                                                             ctgctgATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCat                                                                    >  6:348752/1‑149 (MQ=255)                                                                               gctgATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCTCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTcc                                                                                                      <  4:244810/113‑1 (MQ=255)                                                                               gctgATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCTCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTcc                                                                                                      >  3:244810/1‑113 (MQ=255)                                                                                ctgATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATAATAtt                                                                 >  3:187928/1‑149 (MQ=255)                                                                                 tgATCCAGGGCTTCGACTTCAACTGACCCTGCCACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATAttt                                                                <  8:162811/149‑1 (MQ=255)                                                                                  gATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTc                                                               >  2:98597/1‑149 (MQ=255)                                                                                   aTCCAGGGCTTCGACTTCAACTGACCCTTCAACAGCCAAAAAAAAACCCGCCTCCATTCGGATGCGGG‑TTGTTTTCAGCTCGCAGATAAAATACTTGATCTTGGCTTCCTGGTACACAACGTGCTTACGAACAACCGGATCATATTTCt                                                              >  7:244858/1‑149 (MQ=255)                                                                                      cAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTg                                                            <  5:339311/148‑1 (MQ=255)                                                                                      cAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGa                                                           >  5:34543/1‑149 (MQ=255)                                                                                      cAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTATTGa                                                           <  8:71234/149‑1 (MQ=255)                                                                                               gACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGAttt                                                  >  1:354616/1‑149 (MQ=255)                                                                                                  ttCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTg                                                >  7:22672/1‑148 (MQ=255)                                                                                                        tGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCgggg                                         >  1:210455/1‑149 (MQ=255)                                                                                                          aCCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGgg‑tg                                      <  1:98597/149‑1 (MQ=255)                                                                                                            ccTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCTTATTTCTTGATGTCGATTTTGTCCGGgg‑tggt                                    >  8:324062/1‑149 (MQ=255)                                                                                                             cTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGg‑tggtg                                   >  3:246379/1‑149 (MQ=255)                                                                                                              tGCAACAGACAAAAAAAAACCCGCCTCGTTTCGGATGCGGG‑TTTTTTTCAGTTCGCCGAACAATTAATTGATATTGGCTTCGTTGTAAACCACGTGATTGCGAACACCCGCATCATATTTGTGTATGTCGATTTTTTCCGGGT‑TGTTgc                                  >  4:49548/1‑149 (MQ=255)                                                                                                                cAACAGCCAAAAAAAAACCCGCCTCCTTTAGGATGGGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCGTCCTTGTACACCACGTGCTTGCGAACAACCGGATGATATTTCTTGATCTCGATTTTGTCCGGGg‑gggtgggg                                >  2:288790/1‑146 (MQ=255)                                                                                                                   cAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTg                             <  2:354616/149‑1 (MQ=255)                                                                                                                     gCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGtt                           <  3:49548/149‑1 (MQ=255)                                                                                                                      ccAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTc                          <  5:348752/149‑1 (MQ=255)                                                                                                                       ctgaaaaaaaCCCGCATCCGAAAGGAGGCGGGTTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGgg‑tggt                                    >  6:84743/4‑139 (MQ=37)                                                                                                                       ctgaaaaaaaCCCGCATCCGAAAGGAGGCGGGTTTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGgg‑tggt                                    <  5:84743/136‑1 (MQ=37)                                                                                                                        aaaaaaacaCCATCCTCCTTTCGGATGAGGG‑TTTTTTTCAGGTCGCCGATAAATTAGTTGATCTTGGCTTCCTTGTACACCACGTTCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGGCCGGGG‑GGGTTCGCTTGTTCtt                        <  8:244858/149‑1 (MQ=255)                                                                                                                        aaaaaaaaaCCCGCCTGCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCtt                        <  4:282546/149‑1 (MQ=255)                                                                                                                        aaaaaaaaaCCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCTCCGATCAATTACTTGATCTTGGCTTCCTTGTACTGCACGTGCTTGCGTACATCCGGATCATATTTCTTGATGTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCtt                        <  1:202576/149‑1 (MQ=255)                                                                                                                        aaaaaaaaaCCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCtt                        <  8:144284/149‑1 (MQ=255)                                                                                                                        aaaaaaaaaCCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCtt                        >  1:109287/1‑149 (MQ=255)                                                                                                                         gaaaaaaaCCCGCATCCGAAAGGAGGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTg                       >  6:79755/2‑149 (MQ=39)                                                                                                                               aaCCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTCggtg                 <  6:353566/149‑1 (MQ=255)                                                                                                                               aaCCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTCggtg                 <  6:34543/149‑1 (MQ=255)                                                                                                                                 cccGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTCggtggt               <  8:259396/149‑1 (MQ=255)                                                                                                                                 cccGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTCggtggt               <  1:288790/149‑1 (MQ=255)                                                                                                                                    gCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTCGgtggtg              >  2:2585/1‑147 (MQ=255)                                                                                                                                      ctcctTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTCGGTGGTGTAg           >  8:129266/1‑148 (MQ=255)                                                                                                                                           ttCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTCGGTGGTGTAGAAGTgg     >  2:80806/1‑149 (MQ=255)                                                                                                                                            tCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTCGGTGGTGTAGAAGTgg     <  7:96399/148‑1 (MQ=255)                                                                                                                                              ggATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTTGGTGGTGTAGAAGTGGCCg  <  7:278515/149‑1 (MQ=255)                                                                                                                                              ggATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGAACATATTTCTTGATCTCGATTTTGTCCGGGGCTGGTGCGCTTGTTCTTGTCGGTGGTGTAGAAGTGGc    <  1:63840/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CCGGGTGGTCGGCCCCAACGAAATGTGCACCCGCGAGTACGACGTCGAGCGCATCCAGATCAAGGCCGGTGCCGACCTGCTGATCCAGGGCTTCGACTTCAACTGACCCTGCAACAGCCAAAAAAAAACCCGCCTCCTTTCGGATGCGGG‑TTTTTTTCAGGTCGCCGATCAATTACTTGATCTTGGCTTCCTTGTACACCACGTGCTTGCGAACAACCGGATCATATTTCTTGATCTCGATTTTGTCCGGGG‑TGGTGCGCTTGTTCTTGTCGGTGGTGTAGAAGTGGCCG  >  NC_002947/6030210‑6030499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑