Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,070,426 C→T 6.6% Q73Q (CAG→CAA)  PP_5324 ← two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,070,4260CT 6.6% 226.9 / 3.6 76Q73Q (CAG→CAA) PP_5324two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base C (35/35);  new base T (3/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

GGGCTTTCTTTACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTA  >  NC_002947/6070278‑6070573
                                                                                                                                                    |                                                                                                                                                   
gggCTTTCTTTACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGgctgc                                                                                                                                                     >  6:70339/1‑149 (MQ=255)
 ggCTTTCTTTACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGgctgct                                                                                                                                                    <  1:198119/149‑1 (MQ=255)
   ctttctttACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGa                                                                                                                                                  <  5:278904/149‑1 (MQ=255)
       ctttACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGAcctg                                                                                                                                              >  7:308761/1‑146 (MQ=255)
       ctttACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACgg                                                                                                                                               <  3:370064/148‑1 (MQ=255)
          tACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCa                                                                                                                                             <  5:70339/147‑1 (MQ=255)
            ccTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCa                                                                                                                                          <  2:384273/148‑1 (MQ=255)
             cTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAgg                                                                                                                                        >  2:68728/1‑149 (MQ=255)
                ggTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGGCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAgggc                                                                                                                                      <  8:38793/148‑3 (MQ=255)
                   cagcagTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGc                                                                                                                                   >  1:303710/1‑148 (MQ=255)
                    agcagTTGCTCGTTGGTGAACGGCTTGCCTACAAACTCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCaa                                                                                                                                 <  7:338232/149‑1 (MQ=255)
                    agcagTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCaa                                                                                                                                 <  3:312014/149‑1 (MQ=255)
                      cagTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAAtt                                                                                                                               <  8:325504/149‑1 (MQ=255)
                      cagTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAAtt                                                                                                                               <  2:205021/149‑1 (MQ=255)
                      cagTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAAt                                                                                                                                >  7:325504/1‑148 (MQ=255)
                           gCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCaa                                                                                                                           <  8:239849/148‑1 (MQ=255)
                            cTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGc                                                                                                                         <  6:296216/149‑1 (MQ=255)
                                   gTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACAt                                                                                                                  <  1:68728/149‑1 (MQ=255)
                                      aaCGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTc                                                                                                               <  7:10088/149‑1 (MQ=255)
                                         ggCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCgg                                                                                                            >  3:114067/1‑149 (MQ=255)
                                                cTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTcc                                                                                                     >  1:197263/1‑149 (MQ=255)
                                                cTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTcc                                                                                                     >  4:215979/1‑149 (MQ=255)
                                                 tACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTcc                                                                                                     >  2:323113/1‑148 (MQ=255)
                                                    aaaaTCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGt                                                                                                 >  4:118697/1‑149 (MQ=255)
                                                     aaaTCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGTATGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTc                                                                                                >  2:22312/1‑149 (MQ=255)
                                                     aaaTCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTACAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTc                                                                                                >  5:178521/1‑149 (MQ=255)
                                                      aaTCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCg                                                                                               >  1:297288/1‑149 (MQ=255)
                                                        tCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGca                                                                                             <  3:63184/149‑1 (MQ=255)
                                                          ggAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGcac                                                                                            <  5:360385/148‑1 (MQ=255)
                                                          ggAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGca                                                                                             >  4:160504/1‑147 (MQ=255)
                                                           gAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAg                                                                                          >  4:165160/1‑149 (MQ=255)
                                                            aaaCACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAg                                                                                          >  6:259547/1‑148 (MQ=255)
                                                                   cGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCAtt                                                                                                        >  6:177860/1‑127 (MQ=255)
                                                                   cGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCAtt                                                                                                        <  5:177860/127‑1 (MQ=255)
                                                                           tCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCt                                                                          <  5:44378/149‑1 (MQ=255)
                                                                              ccTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTcc                                                                       >  6:290885/1‑149 (MQ=255)
                                                                              ccTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTcc                                                                       >  8:220520/1‑149 (MQ=255)
                                                                               cTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTATTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCAGAGGACCAGGTCGAACGCTTCCt                                                                      <  4:91511/149‑1 (MQ=255)
                                                                                tGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCtt                                                                     >  8:313646/1‑149 (MQ=255)
                                                                                     gACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGAc                                                                                                                    >  1:188330/1‑97 (MQ=255)
                                                                                     gACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGAc                                                                                                                    <  2:188330/97‑1 (MQ=255)
                                                                                      aCGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGa                                                                <  5:259547/148‑1 (MQ=255)
                                                                                           ctcCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGcatgg                                                          >  7:215553/1‑149 (MQ=255)
                                                                                           ctcCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGcatg                                                           <  5:290885/148‑1 (MQ=255)
                                                                                              cTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGcac                                                                                            <  7:157785/112‑1 (MQ=255)
                                                                                              cTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGcac                                                                                            >  8:157785/1‑112 (MQ=255)
                                                                                              cTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTc                                                                                 <  4:70212/123‑1 (MQ=255)
                                                                                              cTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTc                                                                                 >  3:70212/1‑123 (MQ=255)
                                                                                              cTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGcatggcca                                                       >  7:363578/1‑149 (MQ=255)
                                                                                              cTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGcatggcca                                                       >  7:269767/1‑149 (MQ=255)
                                                                                                 gTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGcatggccat                                                      <  2:228558/147‑1 (MQ=255)
                                                                                                    gCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCt                                                 >  8:266927/1‑149 (MQ=255)
                                                                                                    gCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCt                                                 >  8:87491/1‑149 (MQ=255)
                                                                                                         tgCTGGTCACCATGATGAACTGGATGGTCTTCATTGCAGTCTTGGGACGGCACAAGGTCAGCAATTACAAGCCAGACATTTCCGTCATTTCCCAGTCTCACATGACCAGGTCGAACGCTTCCt                                                                      <  7:214210/122‑1 (MQ=255)
                                                                                                         ggCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGTTGACGGCACCAGGTCAGCAATTCCAAGCCAGGCATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCt                                                                      >  8:219847/1‑123 (MQ=255)
                                                                                                         ggCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGTTGACGGCACCAGGTCAGCAATTCCAAGCCAGGCATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCt                                                                      >  8:214209/1‑123 (MQ=255)
                                                                                                         ggCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGTTGACGGCACCAGGTCAGCAATTCCAAGCCAGCCATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCt                                                                      <  7:219847/123‑1 (MQ=255)
                                                                                                         ggCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGTTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCt                                                                      <  7:214209/123‑1 (MQ=255)
                                                                                                         ggCTGGTCACCATGATGAACTGAAGGTTCTTCAGTGCAGGATGTTGCAGGAACCAGGGCAGCAATTCCAAGCCAGCCAGTTCCGGCAGTTCCACGTCGCACAGGACCAGGTCGAACGCTTCCt                                                                      >  8:214210/1‑123 (MQ=255)
                                                                                                             ggTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGt                                        <  3:118697/149‑1 (MQ=255)
                                                                                                              gTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGt                                        <  3:160504/148‑1 (MQ=255)
                                                                                                                 aCCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGACCATGGCCTTGCGACCGTTGAt                                    <  2:303710/149‑1 (MQ=255)
                                                                                                                  ccATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCTCAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATa                                   <  6:178521/149‑1 (MQ=255)
                                                                                                                    atgatgAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGc                                 <  7:313646/149‑1 (MQ=255)
                                                                                                                      gatgaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGcgt                               >  1:353858/1‑149 (MQ=255)
                                                                                                                        tgaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGcgtcg                             >  3:152556/1‑149 (MQ=255)
                                                                                                                         gaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGt                                        <  3:15667/137‑1 (MQ=255)
                                                                                                                         gaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGt                                        >  4:15667/1‑137 (MQ=255)
                                                                                                                            cTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGa                         >  6:74048/1‑149 (MQ=255)
                                                                                                                               cAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATgg                      <  2:353858/149‑1 (MQ=255)
                                                                                                                               cAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATgg                      >  7:12240/1‑149 (MQ=255)
                                                                                                                                 ggTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGcc                    <  3:165160/149‑1 (MQ=255)
                                                                                                                                 ggTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGcc                    <  2:197263/149‑1 (MQ=255)
                                                                                                                                         aGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCgg            >  3:304348/1‑149 (MQ=255)
                                                                                                                                         aGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCgg            <  2:297288/149‑1 (MQ=255)
                                                                                                                                             cAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAg        >  3:139633/1‑149 (MQ=255)
                                                                                                                                             cAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAg        >  2:319391/1‑149 (MQ=255)
                                                                                                                                             cAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAg        <  1:323113/149‑1 (MQ=255)
                                                                                                                                                gctgctGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTTTTAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCa     <  1:319391/149‑1 (MQ=255)
                                                                                                                                                  tgctgACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCAt    >  4:186816/1‑148 (MQ=255)
                                                                                                                                                   gctgACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGACATACCAGGGAAGGCATTa  >  5:170994/1‑149 (MQ=255)
                                                                                                                                                    ctgACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCAtt   <  7:266927/147‑1 (MQ=255)
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GGGCTTTCTTTACCTTGGTCAGCAGTTGCTCGTTGGTGAACGGCTTGCCTACAAAATCGGAAACACCCGCCTGGATCGCCTGAATGACGTTCTCCTTGTCGCCACGGCTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTA  >  NC_002947/6070278‑6070573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑