Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,080,988	T→A	11.7%	intergenic (+35/+27)	PP_5332 → / ← PP_5333	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,080,988	0	T	A	11.7%	161.6 / 11.4	67	intergenic (+35/+27)	PP_5332/PP_5333	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/31);  new base A (3/5);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

GATGGCAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAG  >  NC_002947/6080841‑6081135                                                                                                                                                    |                                                                                                                                                    gatggCAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCtt                                                                                                                                                    <  1:5329/149‑1 (MQ=255)   tggCAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGc                                                                                                                                                  >  8:90743/1‑149 (MQ=255)    ggCAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGcc                                                                                                                                                 >  1:252890/1‑149 (MQ=255)         cGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGa                                                                                                                                            <  3:289504/149‑1 (MQ=255)          gagtGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                           <  4:306524/146‑1 (MQ=255)           aaTGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCcgcg                                                                                                                                             >  3:42932/1‑146 (MQ=255)           aaTGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                           <  4:42932/148‑1 (MQ=255)            aTGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                         <  8:161437/149‑1 (MQ=255)            aTGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                         <  3:360056/149‑1 (MQ=255)            aTGCTGTGCATCGAGACAGCGAATGTGGGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                         <  7:199460/149‑1 (MQ=255)                     aTCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCgggg                                                                                                                                 >  5:276312/1‑148 (MQ=255)                         agaCAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAAt                                                                                                                             >  1:343301/1‑148 (MQ=255)                             aGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATc                                                                                                                         >  6:175336/1‑148 (MQ=255)                                gAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAg                                                                                                                     <  7:201468/149‑1 (MQ=255)                                     tgtgGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCt                                                                                                                 <  3:216494/148‑1 (MQ=255)                                       tgGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGc                                                                                                               <  1:307138/148‑1 (MQ=255)                                         ggATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTc                                                                                                            >  1:98389/1‑149 (MQ=255)                                          gaggACCTGGTGTAGTTGAAGCCTGGGGCTAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGCCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCt                                                                                                           <  2:334393/146‑1 (MQ=255)                                            tGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTc                                                                                                         >  4:163213/1‑149 (MQ=255)                                            tGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTc                                                                                                         <  4:104822/149‑1 (MQ=255)                                            tGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTc                                                                                                         <  3:242434/149‑1 (MQ=255)                                             gACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTc                                                                                                         >  5:80503/1‑148 (MQ=255)                                               ccTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTTTCAAAGGTCTGCGTCTTccac                                                                                                      >  5:31782/1‑149 (MQ=255)                                                    tggAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCAccc                                                                                                 <  6:276312/149‑1 (MQ=255)                                                     ggAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCt                                                                                                >  8:327693/1‑149 (MQ=255)                                                         ttGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCAcc                                                                                            >  7:118355/1‑149 (MQ=255)                                                           gAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTccacc                                                                                                     <  3:140367/138‑1 (MQ=255)                                                           gAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTccacc                                                                                                     >  4:140367/1‑138 (MQ=255)                                                              gCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTc                                                                                       <  7:90743/149‑1 (MQ=255)                                                               ccTGGGTCGTGTCATTCGCGGGGCGTCCTGATCGGTTGTCAAATTATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGATTAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCt                                                                                      <  8:138719/149‑1 (MQ=255)                                                               ccTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCt                                                                                      <  3:163213/149‑1 (MQ=255)                                                                   gggCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCa                                                                                   <  5:307293/148‑1 (MQ=255)                                                                   gggCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCAt                                                                                  <  2:252890/149‑1 (MQ=255)                                                                        cgTCCTTCGCTGGCCGTCCCGACCGGTTGTGAAAATATCTGACCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCt                                                                                      <  2:126099/139‑1 (MQ=255)                                                                         gTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAgcg                                                                            <  6:80503/149‑1 (MQ=255)                                                                          tCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAgcg                                                                            >  7:193387/1‑148 (MQ=255)                                                                            aTTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCa                                                                          >  3:296419/1‑148 (MQ=255)                                                                             ttCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCa                                                                          >  3:100859/1‑147 (MQ=255)                                                                                      cggccAGATCGGTTGTTAAAATATCTGCGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCAcc                                                                                            <  2:224698/117‑1 (MQ=255)                                                                                      cGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCAcc                                                                                            >  1:224698/1‑120 (MQ=255)                                                                                         ccAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGc                                                            >  8:123951/1‑149 (MQ=255)                                                                                          cAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCCCCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGc                                                            >  4:329137/1‑148 (MQ=255)                                                                                           aGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCa                                                          <  8:118355/149‑1 (MQ=255)                                                                                           aGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCa                                                          <  5:175336/149‑1 (MQ=255)                                                                                                ggTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCa                                                     <  7:327693/149‑1 (MQ=255)                                                                                                 gTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGc                                                                     >  8:271577/1‑132 (MQ=255)                                                                                                 gTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGc                                                                     <  7:271577/132‑1 (MQ=255)                                                                                                      gAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCt                                                >  2:294087/1‑148 (MQ=255)                                                                                                        aaaTATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGAc                                             >  1:142644/1‑149 (MQ=255)                                                                                                              cTGAGACCTGCTCTTTTTTTACCGGCCTCATAGCGGGCATGGCCGCGAAGAGGGCCGGGCAAATATGAAAAGTATGGCTAATGAACCCCCCACCCCGTAACAGCAACCCGCCCATATGCCCGATCGGCCCCGGCATAGCTGACCtt                                          >  2:190195/1‑146 (MQ=255)                                                                                                                 aGCCCTGCTCTTTTTTTCCCGGCCTCTGCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTtctc                                       <  3:161031/146‑1 (MQ=255)                                                                                                                        ctctTTTTTTACCGCCCTCTGCGCGGGCTGGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCa                               <  6:31782/147‑1 (MQ=255)                                                                                                                         tctTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTcc                                      <  1:333498/139‑1 (MQ=255)                                                                                                                         tctTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTcc                                      >  2:333498/1‑139 (MQ=255)                                                                                                                                    cGGCCTCTTCGCGGGCTTGCCGGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGAcc                 <  2:343301/149‑1 (MQ=255)                                                                                                                                    cGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCGTCTCCACCTTCAGCTTGCCGCTGAcc                 >  5:199907/1‑149 (MQ=255)                                                                                                                                      gCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCca               >  5:380789/1‑149 (MQ=255)                                                                                                                                        ctctTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGAc                  <  1:166067/144‑1 (MQ=255)                                                                                                                                        ctctTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGAc                  >  2:166067/1‑144 (MQ=255)                                                                                                                                        ctctTCGCGGGCAAGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCca               >  3:377425/1‑147 (MQ=255)                                                                                                                                         tctTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAg            >  2:261916/1‑149 (MQ=255)                                                                                                                                         tctTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAg            >  8:352701/1‑149 (MQ=255)                                                                                                                                           ttCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCg          >  3:321246/1‑149 (MQ=255)                                                                                                                                              gcgGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCggg        <  6:380789/148‑1 (MQ=255)                                                                                                                                              gcgGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCggg        <  1:261916/148‑1 (MQ=255)                                                                                                                                              gcgGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCggg        <  2:98389/148‑1 (MQ=255)                                                                                                                                                   cTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAg  <  7:123951/149‑1 (MQ=255)                                                                                                                                                   cTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAg  <  1:354825/149‑1 (MQ=255)                                                                                                                                                    ttGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTACAGCTTGCCGCTGACCCACAGCGGGgtg     >  2:196811/1‑145 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GATGGCAGCGAATGCTGTGCATCGAGACAGCGAATGTGTGGGATGACCTGGTGGAGTTGAAGCCTGGGGCGAGTCATTCGCTGGGCGTCCAGATCGGTTGTGAAAATATCTGAGCCCTGCTCTTTTTTTACCGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGGGCAATTATCAAAGGTCTGCGTCTTCCACCACCCTCACCTTGTCTGCATCCAGCGCATAGGCCGCATCGGCCAGGTCATTGCTGACCTTCTCCACCTTCAGCTTGCCGCTGACCCACAGCGGGGTGTAG  >  NC_002947/6080841‑6081135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑