Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,098,715 A→C 5.8% M125R (ATG→AGG) ‡ PP_5350 ← RpiR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,098,7150AC 5.8% 274.6 / 6.4 87M125R (ATG→AGG) ‡PP_5350RpiR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (39/43);  new base C (2/3);  total (41/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

CAGGGTAACGGCCGACATCGCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATGGCGAACTGACCGAAGCTGGCCCCGG  >  NC_002947/6098570‑6098862
                                                                                                                                                   |                                                                                                                                                   
cAGGGTAACGGCCGACATCGCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATg                                                                                                                                                   <  8:202010/148‑1 (MQ=255)
  gggTAACGGGCGACATCGCTTGCATGTGGGGGTGGGAATACGCGTCCTCTCTTAGCAGCTGGGGGAATAAATTTTTCCTCTGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATgg                                                                                                                                                  <  1:314972/149‑1 (MQ=255)
  gggTAACGGCCGACATCGCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGgcg                                                                                                                                                >  3:50625/1‑149 (MQ=255)
  gggTAACGGCCGACATCGCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGgcg                                                                                                                                                <  1:386353/149‑1 (MQ=255)
   ggTAACGGCCGACATCGCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGgcg                                                                                                                                                >  7:146433/1‑148 (MQ=255)
     tAACGGCCGACATCGCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCATGGCCCAGgcgcag                                                                                                                                             <  1:241729/149‑3 (MQ=255)
        cGGCCGACATCGCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCATGGCCCAGgcgcagcgg                                                                                                                                          >  2:78677/1‑144 (MQ=255)
        cGGCCGACATCGCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCATGGCCCAGgcgcagcgg                                                                                                                                          <  1:78677/149‑6 (MQ=255)
          gCCGACATCGCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGc                                                                                                                                        <  4:256726/149‑1 (MQ=255)
                   gCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCa                                                                                                                                <  6:45427/148‑1 (MQ=255)
                   gCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCa                                                                                                                                <  4:50625/148‑1 (MQ=255)
                    cTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCTCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAgc                                                                                                                              <  2:305518/149‑1 (MQ=255)
                     ttGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAgcg                                                                                                                             >  4:181437/1‑149 (MQ=255)
                         aTGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTg                                                                                                                         >  2:345685/1‑149 (MQ=255)
                          tgtgGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGc                                                                                                                        <  1:281845/149‑1 (MQ=255)
                              ggggTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGa                                                                                                                     >  8:295928/1‑148 (MQ=255)
                               gggTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGAt                                                                                                                    >  1:133532/1‑148 (MQ=255)
                               gggTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGAt                                                                                                                    >  6:308767/1‑148 (MQ=255)
                                ggTCGGAATACGCTGCCGCTCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGAACCGGACGCGCCAAAGCCAGAGAAATCTACCCGCTGCGACTGGGCAATGGCGCTCACGGCATGTTGCAGCGCCTGCGGATc                                                                                                                   >  4:243507/1‑148 (MQ=255)
                                  tCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAg                                                                                                                <  6:166615/149‑1 (MQ=255)
                                  tCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAg                                                                                                                <  2:57082/149‑1 (MQ=255)
                                    ggAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGt                                                                                                              <  8:70174/149‑1 (MQ=255)
                                     gAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCtt                                                                                                                                      >  5:251860/1‑124 (MQ=255)
                                     gAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCtt                                                                                                                                      <  6:251860/124‑1 (MQ=255)
                                            cTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCgcgc                                                                                                      <  5:141410/149‑1 (MQ=255)
                                                cgcgCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAGGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGGACtt                                                                                                  >  8:328269/1‑149 (MQ=255)
                                                cgcgCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACtt                                                                                                  <  2:133532/149‑1 (MQ=255)
                                                 gcgcTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCACACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTc                                                                                                 >  6:85079/1‑149 (MQ=255)
                                                  cgcTGAGCAGCATGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTcc                                                                                                <  8:146433/149‑1 (MQ=255)
                                                  cgcTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCGCGCGCACTTcc                                                                                                <  4:236403/149‑1 (MQ=255)
                                                   gcTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCa                                                                                               >  7:184538/1‑149 (MQ=255)
                                                        gcagcaGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAgg                                                                                          <  2:339461/149‑1 (MQ=255)
                                                          agcagGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGgt                                                                                        <  6:6523/149‑1 (MQ=255)
                                                            cagGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTAACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCAGCAGCgtgt                                                                                      >  2:235847/1‑149 (MQ=255)
                                                            cagGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCATGGCCCAGgcgcag                                                                                                                                             >  5:374616/1‑92 (MQ=255)
                                                            cagGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCATGGCCCAGgcgcag                                                                                                                                             <  6:374616/94‑3 (MQ=255)
                                                              ggCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGtgtg                                                                                     >  7:66217/1‑148 (MQ=255)
                                                               gCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCa                                                                                   <  8:56008/149‑1 (MQ=255)
                                                                 ggAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAgg                                                                                 >  6:34907/1‑149 (MQ=255)
                                                                 ggAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAgg                                                                                 >  6:142921/1‑149 (MQ=255)
                                                                  gaa‑gaaCTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAggg                                                                                >  3:263367/1‑149 (MQ=255)
                                                                    a‑gaaCTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCttgttg                                                                                                                                  >  8:360814/1‑97 (MQ=255)
                                                                    a‑gaaCTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCttgttg                                                                                                                                  <  7:360814/97‑1 (MQ=255)
                                                                    a‑gaaCTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGggtg                                                                              <  1:345685/149‑1 (MQ=255)
                                                                    a‑gaaCTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGggtg                                                                              <  3:20942/149‑1 (MQ=255)
                                                                       aaCTTGTGCT‑GGGCATCGGCCGCAACCGGACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGggtggt                                                                            >  1:218268/1‑149 (MQ=255)
                                                                       aaCTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGggtggt                                                                            >  6:227233/1‑149 (MQ=255)
                                                                         cTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGgtgt                                                                          <  7:295928/149‑1 (MQ=255)
                                                                         cTTGTCCT‑GCGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGgtgt                                                                          <  8:122799/149‑1 (MQ=255)
                                                                          ttGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAgg                                                                                          >  7:77487/1‑132 (MQ=255)
                                                                          ttGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAgg                                                                                          <  8:77487/132‑1 (MQ=255)
                                                                               cT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCg                                                                        >  8:346056/1‑145 (MQ=255)
                                                                                  gggCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGaa                                                                      >  8:209335/1‑145 (MQ=255)
                                                                                  gggCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGaa                                                                      <  7:209335/145‑1 (MQ=255)
                                                                                    gCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCtt                                                                >  2:208352/1‑149 (MQ=255)
                                                                                      aTCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCa                                                              <  5:227233/149‑1 (MQ=255)
                                                                                      aTCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCa                                                              <  2:218268/149‑1 (MQ=255)
                                                                                          gCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCt                                                          >  2:184001/1‑149 (MQ=255)
                                                                                           ccGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTg                                                         <  5:142921/149‑1 (MQ=255)
                                                                                            cGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATc                                                                  >  6:168639/1‑139 (MQ=255)
                                                                                            cGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATc                                                                  <  5:168639/139‑1 (MQ=255)
                                                                                            cGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTg                                                         >  1:262938/1‑148 (MQ=255)
                                                                                             gCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTg                                                         >  3:282050/1‑147 (MQ=255)
                                                                                             gCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTa                                                       <  2:244245/149‑1 (MQ=255)
                                                                                             gCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCACCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTg                                                         >  1:105482/1‑147 (MQ=255)
                                                                                                       cGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGAcc                                             <  1:184001/149‑1 (MQ=255)
                                                                                                            gcgcCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATc                                        <  5:308767/149‑1 (MQ=255)
                                                                                                            gcgcCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATc                                        <  3:181437/149‑1 (MQ=255)
                                                                                                            gcgcCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGCGGACCGCATc                                        >  6:93267/1‑149 (MQ=255)
                                                                                                                 aaaGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCtt                                   >  5:118340/1‑149 (MQ=255)
                                                                                                                 aaaGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTAGGCGACCGAATCGTcat                                   >  5:38478/1‑147 (MQ=255)
                                                                                                                   aGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCg                                 <  2:262938/149‑1 (MQ=255)
                                                                                                                     ccATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTg                               >  5:20641/1‑149 (MQ=255)
                                                                                                                     ccATAGAAATCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTAGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCGTCGTg                               >  8:171441/1‑149 (MQ=255)
                                                                                                                      cATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTgg                              <  5:34907/149‑1 (MQ=255)
                                                                                                                         aGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGATGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATg                           >  4:223128/1‑149 (MQ=255)
                                                                                                                          gAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATg                           <  8:184538/148‑1 (MQ=255)
                                                                                                                           aaCTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATGGc                         >  1:213146/1‑149 (MQ=255)
                                                                                                                           aaCTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGGCGGCGACCGAATCGTCTTCGTGGATGGc                         <  7:171441/149‑1 (MQ=255)
                                                                                                                                tACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATGGCGaa                      <  1:208352/147‑1 (MQ=255)
                                                                                                                                tACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATGGCGAACt                    >  2:178157/1‑149 (MQ=255)
                                                                                                                                  cccGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCg                                       <  6:275544/128‑1 (MQ=255)
                                                                                                                                  cccGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCg                                       >  5:275544/1‑128 (MQ=255)
                                                                                                                                  cccGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATGGCGAACTg                   <  6:20641/148‑1 (MQ=255)
                                                                                                                                  cccGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATGGCGAACTGa                  <  4:263367/149‑1 (MQ=255)
                                                                                                                                     gCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGTTGTAGTCGGCGACCGAATCGTCTTCGTGGATGGCGAACTGACCg               <  8:66217/149‑1 (MQ=255)
                                                                                                                                            cTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATGGCGAACTGACCGAAGCTgg        >  2:112438/1‑149 (MQ=255)
                                                                                                                                                   aTGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATGGCGAACTGACCGAAGCTGGCCCCgg  >  3:377120/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGGGTAACGGCCGACATCGCTTGCATGTGGGGGTCGGAATACGCTGCCGCGCTGAGCAGCAGGCGGAA‑GAACTTGTGCT‑GGGCATCGGCCGCAACCGCACCGGACGCGCCAAAGCCATAGAACTCTACCCGCTGCGCCTGGGCCATGGCGCTCACGGCTTGTTGCAGCGCCTGCGGATCAAGGTGCTCGCGCACTTCCATCAGGGTGTGCAGGGTGGTGTCGAAGATCTTCAGGCTGTAGTCGGCGACCGAATCGTCTTCGTGGATGGCGAACTGACCGAAGCTGGCCCCGG  >  NC_002947/6098570‑6098862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑