Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 68,603 C→A 7.5% G224W (GGG→TGG)  PP_0058 ← 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294768,6030CA 7.5% 241.1 / 8.5 80G224W (GGG→TGG) PP_00581‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (38/36);  new base A (4/2);  total (42/38)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-01

CAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTACCGC  >  NC_002947/68456‑68749
                                                                                                                                                   |                                                                                                                                                  
cAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGcccc                                                                                                                                                   <  3:163562/149‑1 (MQ=255)
 aaaaaCTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCg                                                                                                                                                  >  8:187299/1‑149 (MQ=255)
 aaaaaCTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCg                                                                                                                                                  >  6:363632/1‑149 (MQ=255)
     aCTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGcc                                                                                                                                              <  3:71267/149‑1 (MQ=255)
            acaAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCg                                                                                                                                       >  6:361409/1‑149 (MQ=255)
               aGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTAc                                                                                                                                    <  5:361409/149‑1 (MQ=255)
                     ccACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCGATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGGAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTCCAGATGGCGTACATCGGc                                                                                                                              >  8:86395/1‑149 (MQ=255)
                      cacaGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCt                                                                                                                             >  4:97748/1‑149 (MQ=255)
                       acaGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCt                                                                                                                             >  7:251117/1‑148 (MQ=255)
                         aGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCAc                                                                                                                          >  7:61195/1‑149 (MQ=255)
                         aGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCAc                                                                                                                          >  6:56933/1‑149 (MQ=255)
                            aGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCaa                                                                                                                       >  4:151903/1‑149 (MQ=255)
                              gTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTGACCAAt                                                                                                                      >  1:209060/1‑148 (MQ=255)
                              gTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAAt                                                                                                                      >  1:207599/1‑148 (MQ=255)
                              gTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAAt                                                                                                                      >  1:217530/1‑148 (MQ=255)
                                cAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTAc                                                                                                                   >  2:333323/1‑149 (MQ=255)
                                      aTCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGTCGTTGGCGTACATCGGCTCACCAATTACCACTTc                                                                                                             <  2:86811/149‑1 (MQ=255)
                                      aTCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCCATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTc                                                                                                             <  5:363632/149‑1 (MQ=255)
                                        cTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGAAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGACCCGTGCCGGTGGCGGACAACGGCTAACCAATTAACACTTCGa                                                                                                           >  8:283791/1‑149 (MQ=255)
                                           cTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCg                                                                                                            <  2:207599/145‑1 (MQ=255)
                                           cTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGa                                                                                                           <  7:187299/146‑1 (MQ=255)
                                           cTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGAt                                                                                                          <  3:380783/147‑1 (MQ=255)
                                             ccTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGa                                                                                                           >  2:372688/1‑144 (MQ=255)
                                             ccTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGa                                                                                                           <  1:372688/144‑1 (MQ=255)
                                              cTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTAc                                                                                                     >  8:283455/1‑149 (MQ=255)
                                                 aGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCg                                                                                                   <  5:242723/148‑1 (MQ=255)
                                                  ggTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCgg                                                                                                  >  8:92083/1‑148 (MQ=255)
                                                    tttttCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCAc                                                                                               >  5:65050/1‑149 (MQ=255)
                                                    tttttCAGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAAtt                                                                                                                     >  6:37933/1‑127 (MQ=255)
                                                    tttttCAGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAAtt                                                                                                                     <  5:37933/127‑1 (MQ=255)
                                                     ttttCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACg                                                                                              >  3:66197/1‑149 (MQ=255)
                                                      tttCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACg                                                                                              <  2:217530/148‑1 (MQ=255)
                                                       ttCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGc                                                                                             <  3:207405/148‑1 (MQ=255)
                                                         ccGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTg                                                                                          >  7:85689/1‑149 (MQ=255)
                                                           gCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGcc                                                                                        <  3:97748/149‑1 (MQ=255)
                                                            cAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGccc                                                                                       <  6:328340/149‑1 (MQ=255)
                                                            cAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGccc                                                                                       <  7:283455/149‑1 (MQ=255)
                                                            cAGCTGGCGGCAGCGATCCCATCGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGAACGCTTGccc                                                                                       >  6:203091/1‑149 (MQ=255)
                                                                  gcggcAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCg                                                                                 <  5:21549/149‑1 (MQ=255)
                                                                    ggcAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGc                                                                                         <  6:96726/139‑1 (MQ=255)
                                                                    ggcAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGc                                                                                         >  5:96726/1‑139 (MQ=255)
                                                                      cAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCGCCAACCGGt                                                                               >  7:245576/1‑147 (MQ=255)
                                                                      cAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGt                                                                               <  8:245576/147‑1 (MQ=255)
                                                                      cAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCt                                                                             <  6:65050/149‑1 (MQ=255)
                                                                        gCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTg                                                                           <  3:106319/149‑1 (MQ=255)
                                                                         cGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTgg                                                                          <  3:371606/149‑1 (MQ=255)
                                                                           aTCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGc                                                                        >  3:240815/1‑149 (MQ=255)
                                                                           aTCCCATCGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGc                                                                        <  5:203091/149‑1 (MQ=255)
                                                                              ccATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCCACCGGTCTTGGGc                                                                        >  1:371366/1‑146 (MQ=255)
                                                                                aTGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGaa                                                                   <  7:92083/149‑1 (MQ=255)
                                                                                               gCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgtg                                                    <  4:240815/149‑1 (MQ=255)
                                                                                               gCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgt                                                     <  6:181673/148‑1 (MQ=255)
                                                                                                 ttCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTggg                                                  >  7:277485/1‑149 (MQ=255)
                                                                                                        cAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCg                                            <  1:333323/148‑1 (MQ=255)
                                                                                                        cAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGAGCAATc                                             <  6:57388/147‑1 (MQ=255)
                                                                                                        cAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGAGCAATc                                             >  5:57388/1‑147 (MQ=255)
                                                                                                         aaGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCgg                                           >  5:390163/1‑148 (MQ=255)
                                                                                                          aGCTGCAGCGCGGTCGTTGAGTTCTGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGTCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGgca                                         <  5:123744/149‑1 (MQ=255)
                                                                                                               cAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCAcc                                    >  8:250417/1‑149 (MQ=255)
                                                                                                                aGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCAAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCg                                   >  7:42555/1‑149 (MQ=255)
                                                                                                                 gcgcGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCgg                                  <  8:277485/149‑1 (MQ=255)
                                                                                                                   gcgGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTATCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCa                                <  5:56933/149‑1 (MQ=255)
                                                                                                                     ggTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTggg                                                  <  8:182266/129‑1 (MQ=255)
                                                                                                                     ggTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTggg                                                  >  7:182266/1‑129 (MQ=255)
                                                                                                                       tCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGccc                            >  7:191853/1‑149 (MQ=255)
                                                                                                                         gTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCg                           <  8:251117/148‑1 (MQ=255)
                                                                                                                               ttCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTc                     >  2:224481/1‑148 (MQ=255)
                                                                                                                               ttCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCa                    >  4:379164/1‑149 (MQ=255)
                                                                                                                                 cGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCAc                   <  8:85689/148‑1 (MQ=255)
                                                                                                                                   gCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCgg                                  <  7:335213/131‑1 (MQ=255)
                                                                                                                                   gCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGc                >  3:248694/1‑149 (MQ=255)
                                                                                                                                   ccAACGGCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgtg                                                    <  7:45223/112‑1 (MQ=255)
                                                                                                                                   ccAACGGCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgtg                                                    >  8:45223/2‑113 (MQ=255)
                                                                                                                                   ccAACGGCACGGGGCCACGTGCCATTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGc                >  7:193206/2‑149 (MQ=255)
                                                                                                                                    cAACGGCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCAc                   <  2:10593/145‑1 (MQ=255)
                                                                                                                                    cAACGGCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCAc                   >  1:10593/1‑145 (MQ=255)
                                                                                                                                          cACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCg         >  1:82660/1‑149 (MQ=255)
                                                                                                                                           aCGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCgg        >  8:346721/1‑149 (MQ=255)
                                                                                                                                              tGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGGACGCTTGCCCCAACCGGTCATGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTAc     <  7:86395/149‑1 (MQ=255)
                                                                                                                                               ggCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTAcc    >  8:268835/1‑149 (MQ=255)
                                                                                                                                                gCCCGGTGCCGTTGGCGTACATCGGCTCACCAATTAACACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTAgcg   <  7:283791/149‑1 (MQ=255)
                                                                                                                                                 ccccGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTAccgc  >  5:110017/1‑149 (MQ=255)
                                                                                                                                                 ccccGGGCCGTTGGCGTACATCGGCTCACCAATTCCCACTTCGATTGTACCGGCACGCCTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTAccgc  <  8:61195/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGTACCGC  >  NC_002947/68456‑68749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑