Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,129,170	C→T	6.2%	intergenic (+32/+35)	PP_5376 → / ← PP_5377	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,129,170	0	C	T	6.2%	240.5 / 4.3	81	intergenic (+32/+35)	PP_5376/PP_5377	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (38/37);  new base T (2/3);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

TCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCA  >  NC_002947/6129022‑6129317                                                                                                                                                        |                                                                                                                                                     tCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGcc                                                                                                                                                      >  5:193036/1‑149 (MQ=255)      ccGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTaa                                                                                                                                                  >  8:323099/1‑148 (MQ=255)       cGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTaaaa                                                                                                                                                <  2:293422/149‑1 (MQ=255)               cTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGtt                                                                                                                                        >  5:67467/1‑149 (MQ=255)                  tgACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTg                                                                                                                                      <  2:303915/148‑1 (MQ=255)                     cGTGATTCCTGCGCAAATGACCTATTAAGACTCGACAGGCAAGATCAACATTCTCGAATACCTCGCAGCTGGAATCAATTGCCGAGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGCAAACGGCGCTTTTAAGGCGCCGTTTTCGtt                                                                                                                                  <  4:224631/149‑1 (MQ=38)                      gggctGCCTGCGCAAATGACCTATGAAGACTCGACAGCCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTg                                                                                                                                 <  8:128340/145‑1 (MQ=255)                      gTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAC‑CGCAAACGGCGCTTTTAAGGCGCCGTTTTCGttt                                                                                                                                 <  5:169161/149‑2 (MQ=255)                        gATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTgg                                                                                                                                >  3:61798/1‑148 (MQ=255)                        gATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTg                                                                                                                                 <  1:333776/147‑1 (MQ=255)                         aTGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCAGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTggg                                                                                                                               >  7:172837/1‑148 (MQ=255)                           gCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGaa                                                                                                                             >  1:267644/1‑148 (MQ=255)                            ccTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGaaa‑a                                                                                                                          >  8:330225/1‑149 (MQ=255)                                 gcAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTg                                                                                                                     <  6:235365/149‑1 (MQ=255)                                  cAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTgg                                                                                                                    <  4:168604/149‑1 (MQ=255)                                   aaaTGACCTATTAATACTCGAAACGCAAGCTCAACATTCTAGTATACCGCGCACCTTGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTTAATTCTGTCCAAATCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTgg                                                                                                                    <  1:280816/149‑1 (MQ=255)                                   aaaTGACCTATGAAGACTCGCCAGGCAAGCTCAACATTCTCGAATACCGCGCCGCTGGCATGAAGTGCCGCGGGCA‑‑AAAGTGCGCGCTGAATGCTCTCCACA‑CGAAAACGGCGCAGTACAAGCGCCGTTTCAGTTGGGACG‑ACGGAggg                                                                                                                   >  2:327072/1‑149 (MQ=255)                                   aaaTGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTggg                                                                                                                   >  2:18614/1‑149 (MQ=255)                                             tGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGc                                                                                                           <  2:267644/147‑1 (MQ=255)                                             tGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGc                                                                                                           <  7:103276/147‑1 (MQ=255)                                                aGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGa                                                                                                      <  6:382796/149‑1 (MQ=255)                                                   cTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATAc                                                                                                   >  5:137401/1‑149 (MQ=255)                                                      gACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAGGAAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATAct                                                                                                  >  1:300644/1‑149 (MQ=255)                                                         aGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGTCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTg                                                                                                             <  5:47473/133‑1 (MQ=255)                                                         aGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGTCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTg                                                                                                             >  6:47473/1‑133 (MQ=255)                                                         aGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGttt                                                                                                                                       >  2:361238/1‑108 (MQ=255)                                                              aGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCAc                                                                                        <  3:364384/149‑1 (MQ=255)                                                                 tCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACtt                                                                                      >  7:197966/1‑148 (MQ=255)                                                                 tCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACtt                                                                                      >  1:93861/1‑148 (MQ=255)                                                                   aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCAt                                                                                   >  7:95741/1‑149 (MQ=255)                                                                   aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCAt                                                                                   >  5:360709/1‑149 (MQ=255)                                                                   aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCAt                                                                                   >  8:292380/1‑149 (MQ=255)                                                                     cATTCACGTATACCGCGCACCTGTCATTAATTGCGTCGGCCA‑‑TAATTGAGCTCTGATAGGTGTCCACA‑CGAAAACCGCGCCTTAAAAGCGCCGTTTGCGTTGGTATA‑CCGGTGGGGCAGTGGCCTGGATACTCTTCAACGTCTTCa                                                                                    <  8:185656/146‑1 (MQ=255)                                                                       ttCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCg                                                                               <  7:292380/149‑1 (MQ=255)                                                                        tctcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAACACGGTGGGTCAGTGGCCTGGATActc                                                                                                 <  1:2975/131‑1 (MQ=255)                                                                        tctcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATActc                                                                                                 >  2:2975/1‑130 (MQ=255)                                                                          tcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGt                                                                                                              >  6:362036/1‑115 (MQ=255)                                                                          tcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGt                                                                                                              <  5:362036/115‑1 (MQ=255)                                                                          tcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTcc                                                                            >  4:169658/1‑149 (MQ=255)                                                                            gAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGc                                                                          >  4:352413/1‑149 (MQ=255)                                                                                   gcgcAGCTGGCATCAATTGCCGCTGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgtt                                                                   <  3:352413/149‑1 (MQ=255)                                                                                       aGCTTGCATCAATTGCCGCTGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTga                                                                 <  5:207563/147‑1 (MQ=255)                                                                                        gCTGGCATCAATTGCCGCGGACA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCTGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGAc                                                              <  4:37313/149‑1 (MQ=255)                                                                                            gCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGa                                                           <  6:137401/148‑1 (MQ=255)                                                                                            gCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGAt                                                          <  6:360709/149‑1 (MQ=255)                                                                                            gCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGAt                                                          <  4:61798/149‑1 (MQ=255)                                                                                                 aaTTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGAcc                                                      <  1:18614/148‑1 (MQ=255)                                                                                                  aTTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAACCGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTg                                                    <  2:300644/149‑1 (MQ=255)                                                                                                  aTTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTg                                                    <  8:95741/149‑1 (MQ=255)                                                                                                   ttGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGagtcgg                                                   >  1:333897/1‑144 (MQ=255)                                                                                                       cgcgGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATg                                               >  2:267406/1‑149 (MQ=255)                                                                                                       cgcgGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATg                                               >  3:295861/1‑149 (MQ=255)                                                                                                            ccA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCAt                                            >  1:177339/1‑147 (MQ=255)                                                                                                            ccA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCAt                                            >  3:145140/1‑147 (MQ=255)                                                                                                            ccA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATga                                          <  4:295861/149‑1 (MQ=255)                                                                                                             cA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCAt                                            >  3:307398/1‑146 (MQ=255)                                                                                                             cA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGAACGAGGACGTGGTATGCAt                                            >  8:124701/1‑146 (MQ=255)                                                                                                             cA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACAAATTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTAGGCaa                                            >  3:283266/1‑145 (MQ=255)                                                                                                              a‑‑aaaTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgaga                                        <  1:267406/149‑1 (MQ=255)                                                                                                              a‑‑aaaTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACCGTCGGTCAGAGGCCTGGATACTCAGCAACCACTTCATCCCGTCCCCTCTTGTTGAGACCGAAGACCTGCTATGCATga                                          >  1:388222/1‑147 (MQ=255)                                                                                                                 aaaTTGATCTCTGAATGCTGTCCAAA‑CGTATTCGGCGCCTTAAATGCGCGGTTTGCGTTGGGAAA‑ACGGTGGGTGAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTATTGTTGAGACCGATGAGCTGGTATGCATgagt                                        <  2:388222/148‑2 (MQ=255)                                                                                                                 aaaTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCGGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgagag                                       <  3:239401/149‑1 (MQ=255)                                                                                                                  aaTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATga                                          >  1:55709/1‑145 (MQ=255)                                                                                                                  aaTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATga                                          <  2:55709/145‑1 (MQ=255)                                                                                                                   aTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCGGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGac                                     <  6:67467/149‑1 (MQ=255)                                                                                                                       aGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCa                                 <  6:193036/149‑1 (MQ=255)                                                                                                                               aTGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGGCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACtt                          <  8:172837/148‑1 (MQ=255)                                                                                                                                  cTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATcc                              >  3:218989/1‑141 (MQ=255)                                                                                                                                  cTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATcc                              <  4:218989/141‑1 (MQ=255)                                                                                                                                       ccaa‑CGCAAACGGCGCTTTTAAGGCGCCGTTTTCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTg                 >  5:172236/3‑149 (MQ=39)                                                                                                                                       cAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCt                  <  2:177339/148‑1 (MQ=255)                                                                                                                                       cAAA‑CGAAAACGGCGCCTTAAAAGAGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTATTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTg                 >  5:145014/1‑149 (MQ=255)                                                                                                                                        aaa‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCTACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTg                 >  3:221941/1‑148 (MQ=255)                                                                                                                                        aaa‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCACCCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTg                 >  5:113908/1‑148 (MQ=255)                                                                                                                                        aaa‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACATg                 >  3:108994/1‑148 (MQ=255)                                                                                                                                            cGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAAGCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACAATCCACTTCCATACCTGga               >  7:306814/1‑147 (MQ=255)                                                                                                                                             gAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGagag            >  8:142171/1‑149 (MQ=255)                                                                                                                                                   ggCGCTTTTAAGGCGCCGTTTTCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATc                               <  3:371238/124‑1 (MQ=255)                                                                                                                                                   ggCGCTTTTAAGGCGCCGTTTTCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATc                               >  4:371238/1‑124 (MQ=255)                                                                                                                                                    gcgcCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCg     <  4:145140/149‑1 (MQ=255)                                                                                                                                                    gcgcCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCg     <  4:307398/149‑1 (MQ=255)                                                                                                                                                      gcCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCCCTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCgg    >  1:262369/1‑148 (MQ=255)                                                                                                                                                       ccTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCa  <  8:197966/149‑1 (MQ=255)                                                                                                                                                        cTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCa  >  8:83542/1‑148 (MQ=255)                                                                                                                                                        |                                                                                                                                                     TCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCA  >  NC_002947/6129022‑6129317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑