Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,129,186	G→T	5.8%	intergenic (+48/+19)	PP_5376 → / ← PP_5377	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,129,186	0	G	T	5.8%	270.8 / 5.9	86	intergenic (+48/+19)	PP_5376/PP_5377	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (39/41);  new base T (2/3);  total (42/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01

TGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTGGGACGGCGA  >  NC_002947/6129039‑6129332                                                                                                                                                       |                                                                                                                                                    tgACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTg                                                                                                                                                     <  2:303915/148‑1 (MQ=255)    cGTGATTCCTGCGCAAATGACCTATTAAGACTCGACAGGCAAGATCAACATTCTCGAATACCTCGCAGCTGGAATCAATTGCCGAGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGCAAACGGCGCTTTTAAGGCGCCGTTTTCGtt                                                                                                                                                 <  4:224631/149‑1 (MQ=38)     gggctGCCTGCGCAAATGACCTATGAAGACTCGACAGCCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTg                                                                                                                                                <  8:128340/145‑1 (MQ=255)     gTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAC‑CGCAAACGGCGCTTTTAAGGCGCCGTTTTCGttt                                                                                                                                                <  5:169161/149‑2 (MQ=255)       gATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTgg                                                                                                                                               >  3:61798/1‑148 (MQ=255)       gATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTg                                                                                                                                                <  1:333776/147‑1 (MQ=255)        aTGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCAGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTggg                                                                                                                                              >  7:172837/1‑148 (MQ=255)          gCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGaa                                                                                                                                            >  1:267644/1‑148 (MQ=255)           ccTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGaaa‑a                                                                                                                                         >  8:330225/1‑149 (MQ=255)                gcAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTg                                                                                                                                    <  6:235365/149‑1 (MQ=255)                 cAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTgg                                                                                                                                   <  4:168604/149‑1 (MQ=255)                  aaaTGACCTATTAATACTCGAAACGCAAGCTCAACATTCTAGTATACCGCGCACCTTGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTTAATTCTGTCCAAATCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTgg                                                                                                                                   <  1:280816/149‑1 (MQ=255)                  aaaTGACCTATGAAGACTCGCCAGGCAAGCTCAACATTCTCGAATACCGCGCCGCTGGCATGAAGTGCCGCGGGCA‑‑AAAGTGCGCGCTGAATGCTCTCCACA‑CGAAAACGGCGCAGTACAAGCGCCGTTTCAGTTGGGACG‑ACGGAggg                                                                                                                                  >  2:327072/1‑149 (MQ=255)                  aaaTGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTggg                                                                                                                                  >  2:18614/1‑149 (MQ=255)                            tGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGc                                                                                                                          <  7:103276/147‑1 (MQ=255)                            tGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGc                                                                                                                          <  2:267644/147‑1 (MQ=255)                               aGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGa                                                                                                                     <  6:382796/149‑1 (MQ=255)                                  cTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATAc                                                                                                                  >  5:137401/1‑149 (MQ=255)                                     gACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAGGAAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATAct                                                                                                                 >  1:300644/1‑149 (MQ=255)                                        aGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGTCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTg                                                                                                                            >  6:47473/1‑133 (MQ=255)                                        aGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGTCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTg                                                                                                                            <  5:47473/133‑1 (MQ=255)                                             aGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCAc                                                                                                       <  3:364384/149‑1 (MQ=255)                                                tCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACtt                                                                                                     >  7:197966/1‑148 (MQ=255)                                                tCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACtt                                                                                                     >  1:93861/1‑148 (MQ=255)                                                  aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCAt                                                                                                  >  7:95741/1‑149 (MQ=255)                                                  aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCAt                                                                                                  >  8:292380/1‑149 (MQ=255)                                                  aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCAt                                                                                                  >  5:360709/1‑149 (MQ=255)                                                    cATTCACGTATACCGCGCACCTGTCATTAATTGCGTCGGCCA‑‑TAATTGAGCTCTGATAGGTGTCCACA‑CGAAAACCGCGCCTTAAAAGCGCCGTTTGCGTTGGTATA‑CCGGTGGGGCAGTGGCCTGGATACTCTTCAACGTCTTCa                                                                                                   <  8:185656/146‑1 (MQ=255)                                                      ttCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCg                                                                                              <  7:292380/149‑1 (MQ=255)                                                       tctcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAACACGGTGGGTCAGTGGCCTGGATActc                                                                                                                <  1:2975/131‑1 (MQ=255)                                                       tctcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATActc                                                                                                                >  2:2975/1‑130 (MQ=255)                                                         tcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGt                                                                                                                             >  6:362036/1‑115 (MQ=255)                                                         tcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGt                                                                                                                             <  5:362036/115‑1 (MQ=255)                                                         tcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTcc                                                                                           >  4:169658/1‑149 (MQ=255)                                                           gAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGc                                                                                         >  4:352413/1‑149 (MQ=255)                                                                  gcgcAGCTGGCATCAATTGCCGCTGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgtt                                                                                  <  3:352413/149‑1 (MQ=255)                                                                      aGCTTGCATCAATTGCCGCTGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTga                                                                                <  5:207563/147‑1 (MQ=255)                                                                       gCTGGCATCAATTGCCGCGGACA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCTGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGAc                                                                             <  4:37313/149‑1 (MQ=255)                                                                           gCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGa                                                                          <  6:137401/148‑1 (MQ=255)                                                                           gCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGAt                                                                         <  4:61798/149‑1 (MQ=255)                                                                           gCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGAt                                                                         <  6:360709/149‑1 (MQ=255)                                                                                aaTTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGAcc                                                                     <  1:18614/148‑1 (MQ=255)                                                                                 aTTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAACCGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTg                                                                   <  2:300644/149‑1 (MQ=255)                                                                                 aTTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTg                                                                   <  8:95741/149‑1 (MQ=255)                                                                                  ttGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGagtcgg                                                                  >  1:333897/1‑144 (MQ=255)                                                                                      cgcgGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATg                                                              >  2:267406/1‑149 (MQ=255)                                                                                      cgcgGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATg                                                              >  3:295861/1‑149 (MQ=255)                                                                                           ccA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCAt                                                           >  3:145140/1‑147 (MQ=255)                                                                                           ccA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCAt                                                           >  1:177339/1‑147 (MQ=255)                                                                                           ccA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATga                                                         <  4:295861/149‑1 (MQ=255)                                                                                            cA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCAt                                                           >  3:307398/1‑146 (MQ=255)                                                                                            cA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGAACGAGGACGTGGTATGCAt                                                           >  8:124701/1‑146 (MQ=255)                                                                                            cA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACAAATTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTAGGCaa                                                           >  3:283266/1‑145 (MQ=255)                                                                                             a‑‑aaaTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgaga                                                       <  1:267406/149‑1 (MQ=255)                                                                                             a‑‑aaaTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACCGTCGGTCAGAGGCCTGGATACTCAGCAACCACTTCATCCCGTCCCCTCTTGTTGAGACCGAAGACCTGCTATGCATga                                                         >  1:388222/1‑147 (MQ=255)                                                                                                aaaTTGATCTCTGAATGCTGTCCAAA‑CGTATTCGGCGCCTTAAATGCGCGGTTTGCGTTGGGAAA‑ACGGTGGGTGAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTATTGTTGAGACCGATGAGCTGGTATGCATgagt                                                       <  2:388222/148‑2 (MQ=255)                                                                                                aaaTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCGGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgagag                                                      <  3:239401/149‑1 (MQ=255)                                                                                                 aaTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATga                                                         >  1:55709/1‑145 (MQ=255)                                                                                                 aaTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATga                                                         <  2:55709/145‑1 (MQ=255)                                                                                                  aTTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCGGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGac                                                    <  6:67467/149‑1 (MQ=255)                                                                                                      aGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCa                                                <  6:193036/149‑1 (MQ=255)                                                                                                              aTGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGGCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACtt                                         <  8:172837/148‑1 (MQ=255)                                                                                                                 cTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATcc                                             <  4:218989/141‑1 (MQ=255)                                                                                                                 cTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATcc                                             >  3:218989/1‑141 (MQ=255)                                                                                                                      ccaa‑CGCAAACGGCGCTTTTAAGGCGCCGTTTTCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTg                                >  5:172236/3‑149 (MQ=39)                                                                                                                      cAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCt                                 <  2:177339/148‑1 (MQ=255)                                                                                                                      cAAA‑CGAAAACGGCGCCTTAAAAGAGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTATTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTg                                >  5:145014/1‑149 (MQ=255)                                                                                                                       aaa‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCTACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTg                                >  3:221941/1‑148 (MQ=255)                                                                                                                       aaa‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCACCCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTg                                >  5:113908/1‑148 (MQ=255)                                                                                                                       aaa‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACATg                                >  3:108994/1‑148 (MQ=255)                                                                                                                           cGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAAGCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACAATCCACTTCCATACCTGga                              >  7:306814/1‑147 (MQ=255)                                                                                                                            gAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGagag                           >  8:142171/1‑149 (MQ=255)                                                                                                                                  ggCGCTTTTAAGGCGCCGTTTTCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATc                                              >  4:371238/1‑124 (MQ=255)                                                                                                                                  ggCGCTTTTAAGGCGCCGTTTTCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATc                                              <  3:371238/124‑1 (MQ=255)                                                                                                                                   gcgcCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCg                    <  4:307398/149‑1 (MQ=255)                                                                                                                                   gcgcCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCg                    <  4:145140/149‑1 (MQ=255)                                                                                                                                     gcCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCCCTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCgg                   >  1:262369/1‑148 (MQ=255)                                                                                                                                      ccTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCa                 <  8:197966/149‑1 (MQ=255)                                                                                                                                       cTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCa                 >  8:83542/1‑148 (MQ=255)                                                                                                                                        ttAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATa               >  1:29229/1‑149 (MQ=255)                                                                                                                                             aGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTgg          <  2:93861/149‑1 (MQ=255)                                                                                                                                             aGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTgg          <  3:169658/149‑1 (MQ=255)                                                                                                                                              gcgcCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTgg          >  3:352626/1‑148 (MQ=255)                                                                                                                                               cgcCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTGGGa        <  7:330225/149‑1 (MQ=255)                                                                                                                                               cgcCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTGGGa        <  7:124701/149‑1 (MQ=255)                                                                                                                                                   gTTTGCGTTGGGAAA‑ACGGGGGGTCAGTGGCCTGGATACTCTGCAGCCACTTCATCCAGTCCGCTCTTGTTGAGCCCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTGGGACGGc    >  8:147656/1‑149 (MQ=255)                                                                                                                                                      tGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTGGGACGGCGa  <  6:145014/148‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                    TGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCA‑‑AAATTGAGCTCTGAATGCTGTCCAAA‑CGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAA‑ACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTGGGACGGCGA  >  NC_002947/6129039‑6129332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑