Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,152,624	C→T	8.1%	R69* (CGA→TGA) ‡	PP_5738 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,152,624	0	C	T	8.1%	239.1 / 8.4	86	R69* (CGA→TGA) ‡	PP_5738	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (40/39);  new base T (4/3);  total (44/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-02

GAGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGC  >  NC_002947/6152477‑6152768                                                                                                                                                    |                                                                                                                                                  gAGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGAcc                                                                                                                                                   >  8:50697/1‑148 (MQ=255)  aGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGa                                                                                                                                                 <  5:340274/149‑1 (MQ=255)  aGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGAGGAGTCCAAAGCTCCGAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGAcc                                                                                                                                                   >  1:320664/1‑147 (MQ=255)     gATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTc                                                                                                                                              <  6:32149/149‑1 (MQ=255)     gATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTc                                                                                                                                              <  8:234762/149‑1 (MQ=255)       ttGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGa                                                                                                                                            >  5:45590/1‑149 (MQ=255)          gCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACTCGGTCGACat                                                                                                                                         <  8:325665/149‑2 (MQ=255)          gCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAg                                                                                                                                         >  1:140768/1‑149 (MQ=255)             aGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAgg                                                                                                                                        >  6:221254/1‑147 (MQ=255)                ttGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGtt                                                                                                                                   >  5:95418/1‑149 (MQ=255)                ttGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGtt                                                                                                                                   >  5:90021/1‑149 (MQ=255)                ttGCCTGGCCAAGTCCGCGTATTTACCTACAACAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGtt                                                                                                                                   >  1:388573/1‑149 (MQ=255)                    cTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTTTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                               >  1:112671/1‑149 (MQ=255)                    cTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                               >  1:379607/1‑149 (MQ=255)                    cTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGATGGCACTCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                               <  8:133320/149‑1 (MQ=255)                     tGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTg                                                                                                                              >  8:269463/1‑149 (MQ=255)                       gCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGc                                                                                                                             <  6:288365/148‑1 (MQ=255)                       gCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑g                                                                                                                           <  7:206483/149‑1 (MQ=255)                        ccAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑gg                                                                                                                          >  3:100593/1‑149 (MQ=255)                        ccAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑gg                                                                                                                          <  4:14025/149‑1 (MQ=255)                         cAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGt                                                                                                                         <  2:112671/149‑1 (MQ=255)                                    aTTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCAc                                                                                                              >  1:160952/1‑149 (MQ=255)                                        aCCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                               <  6:125242/129‑1 (MQ=255)                                           tACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑g                                                                                                                           >  4:189216/1‑129 (MQ=255)                                               gcagAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAaagaa                                                                                                   <  2:165247/149‑1 (MQ=255)                                                 agagagCTGGCACGCTTCGTTTCTTAGCGTGTAGTCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTTATAACGCGCCAAGATTTAGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAgg                                                                                                 <  1:251576/149‑1 (MQ=255)                                                 agagagCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAgg                                                                                                 >  8:95130/1‑149 (MQ=255)                                                  gagagCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAggc                                                                                                <  4:100593/149‑1 (MQ=255)                                                    gagcgcgCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAggcgg                                                                                              <  8:244060/143‑1 (MQ=255)                                                    gagCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAggcg                                                                                               <  7:269463/148‑1 (MQ=255)                                                       cttgCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCa                                                                                            >  4:137560/4‑148 (MQ=255)                                                       cTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCaa                                                                                           >  7:44716/1‑149 (MQ=255)                                                                gtcgtTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCtcgtcgt                                                                                  >  7:70822/1‑149 (MQ=255)                                                                   gtTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGa                                                                               <  8:214795/149‑1 (MQ=255)                                                                       cTGAGCGTGTAGGCCATGTCACGCAAGACTATATGCCAGAGAAGTCCGCAGCTCTTATAACGCGCCAAGATGTCGACCGAGTCTACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGActc                                                                            <  4:210020/148‑1 (MQ=255)                                                                       cTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGActc                                                                            <  5:221254/148‑1 (MQ=255)                                                                          aGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTAGTCGACTCTCAc                                                                        >  5:393356/1‑149 (MQ=255)                                                                           gCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCAcc                                                                       <  6:45590/149‑1 (MQ=255)                                                                              tgtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCgg                                                                    <  7:50697/149‑1 (MQ=255)                                                                              tgtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCgg                                                                    <  2:379607/149‑1 (MQ=255)                                                                              tgtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCgg                                                                    <  4:12038/149‑1 (MQ=255)                                                                                   gCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTAc                                                                >  6:26450/1‑148 (MQ=255)                                                                                     cAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                             >  3:373750/1‑149 (MQ=255)                                                                                     cAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                             <  8:9286/149‑1 (MQ=255)                                                                                     cAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                             >  3:171446/1‑149 (MQ=255)                                                                                      aaGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCATGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                             <  1:317212/148‑1 (MQ=255)                                                                                      aaGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCCGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCCCCCGGTGACGa                                                              >  2:360932/1‑147 (MQ=255)                                                                                      aaGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                             >  2:317212/1‑148 (MQ=255)                                                                                           aCGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGaaa                                                        <  4:171446/148‑1 (MQ=255)                                                                                             gCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACAt                                                     >  4:16387/1‑149 (MQ=255)                                                                                               aaGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTc                                                   >  6:109303/1‑149 (MQ=255)                                                                                                aGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCg                                                  <  3:16387/149‑1 (MQ=255)                                                                                                 gACGATATGCGAGAGAAGTCCGCAGCTATGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCgg                                                 >  1:210051/1‑149 (MQ=255)                                                                                                  aCGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGt                                                <  2:320664/149‑1 (MQ=255)                                                                                                    gATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGtt                                               >  7:121915/1‑148 (MQ=255)                                                                                                    gATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTAAGATCGAAACATTCGGtt                                               >  4:223581/1‑148 (MQ=255)                                                                                                      tatGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCa                                            <  8:70822/149‑1 (MQ=255)                                                                                                        tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAgg                                          >  5:215525/1‑149 (MQ=255)                                                                                                        tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAg                                           <  8:121915/148‑1 (MQ=255)                                                                                                        tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAg                                           <  5:147773/148‑1 (MQ=255)                                                                                                        tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAg                                           <  2:160952/148‑1 (MQ=255)                                                                                                         gCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGAGGCAATCTCGTCGTCGACTCTCACCCCGTTACGATCGAAACATTCGGTTTCAggg                                         >  2:55400/1‑149 (MQ=255)                                                                                                          cGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGt                                        >  5:87100/1‑149 (MQ=255)                                                                                                           gagagaAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGt                                        >  2:196592/1‑148 (MQ=255)                                                                                                                    ccGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTCCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                              <  6:95418/149‑1 (MQ=255)                                                                                                                     cGCAGCTCTGATAACGCGCCAAGATGTCGACCGCGTCGACAGGGAGTTGATTGC‑GGTGGCGTCAGCATTCAAAAAAGACGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                              <  6:90021/148‑1 (MQ=255)                                                                                                                     cGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTg                             <  8:44716/149‑1 (MQ=255)                                                                                                                             tGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAgcg                     >  1:201834/1‑149 (MQ=255)                                                                                                                              gATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                              <  5:379545/139‑1 (MQ=255)                                                                                                                              gATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                              >  6:379545/1‑139 (MQ=255)                                                                                                                               aTAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAgc                      <  6:393356/146‑1 (MQ=255)                                                                                                                                tAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑TGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCa                   <  1:55400/148‑1 (MQ=255)                                                                                                                                tAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAg                  >  4:156144/1‑149 (MQ=255)                                                                                                                                  aCGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGtt                >  6:257307/1‑149 (MQ=255)                                                                                                                                  aCGCGACAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTAACCCTGTTTCGATCGAAACAATCGGTTTCATGTTGACGCCATTTGCAAAAGCGCAttt                >  8:89734/1‑149 (MQ=255)                                                                                                                                    gcgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTg               >  8:231548/1‑148 (MQ=255)                                                                                                                                    gcgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTg               >  8:190962/1‑148 (MQ=255)                                                                                                                                     cgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCa             <  2:201834/149‑1 (MQ=255)                                                                                                                                     cgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCa             <  6:87100/149‑1 (MQ=255)                                                                                                                                        cAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCACACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGa          >  1:340477/1‑149 (MQ=255)                                                                                                                                           cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGa                                      <  5:143444/116‑1 (MQ=255)                                                                                                                                           cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGa                                      >  6:143444/3‑118 (MQ=255)                                                                                                                                           cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCt       >  2:201595/3‑149 (MQ=255)                                                                                                                                           cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCt       >  6:222846/3‑149 (MQ=255)                                                                                                                                            ctGTCGACTCGGTCGACAGGGAGTTGATTGCGGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAgg                                          >  1:243531/2‑114 (MQ=255)                                                                                                                                            ctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAgg                                          <  2:243531/112‑1 (MQ=255)                                                                                                                                                 gACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc  <  5:109303/148‑1 (MQ=255)                                                                                                                                                  aCCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc  >  7:209263/1‑147 (MQ=255)                                                                                                                                                   ccGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc  >  5:154389/1‑146 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GAGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGC  >  NC_002947/6152477‑6152768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑