Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,152,625 G→C 7.9% R69P (CGA→CCA) ‡ PP_5738 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,152,6250GC 7.9% 254.2 / 9.8 87R69P (CGA→CCA) ‡PP_5738hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (41/39);  new base C (4/3);  total (45/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-02

AGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGCCAGAC  >  NC_002947/6152478‑6152773
                                                                                                                                                   |                                                                                                                                                     
aGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGa                                                                                                                                                      <  5:340274/149‑1 (MQ=255)
   gATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTc                                                                                                                                                   <  8:234762/149‑1 (MQ=255)
   gATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTc                                                                                                                                                   <  6:32149/149‑1 (MQ=255)
     ttGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGa                                                                                                                                                 >  5:45590/1‑149 (MQ=255)
        gCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACTCGGTCGACat                                                                                                                                              <  8:325665/149‑2 (MQ=255)
        gCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAg                                                                                                                                              >  1:140768/1‑149 (MQ=255)
           aGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAgg                                                                                                                                             >  6:221254/1‑147 (MQ=255)
              ttGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGtt                                                                                                                                        >  5:90021/1‑149 (MQ=255)
              ttGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGtt                                                                                                                                        >  5:95418/1‑149 (MQ=255)
              ttGCCTGGCCAAGTCCGCGTATTTACCTACAACAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGtt                                                                                                                                        >  1:388573/1‑149 (MQ=255)
                  cTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTTTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                                    >  1:112671/1‑149 (MQ=255)
                  cTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                                    >  1:379607/1‑149 (MQ=255)
                  cTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGATGGCACTCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                                    <  8:133320/149‑1 (MQ=255)
                   tGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTg                                                                                                                                   >  8:269463/1‑149 (MQ=255)
                     gCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGc                                                                                                                                  <  6:288365/148‑1 (MQ=255)
                     gCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑g                                                                                                                                <  7:206483/149‑1 (MQ=255)
                      ccAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑gg                                                                                                                               >  3:100593/1‑149 (MQ=255)
                      ccAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑gg                                                                                                                               <  4:14025/149‑1 (MQ=255)
                       cAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGt                                                                                                                              <  2:112671/149‑1 (MQ=255)
                                  aTTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCAc                                                                                                                   >  1:160952/1‑149 (MQ=255)
                                      aCCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGAtt                                                                                                                                    <  6:125242/129‑1 (MQ=255)
                                         tACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑g                                                                                                                                >  4:189216/1‑129 (MQ=255)
                                             gcagAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAaagaa                                                                                                        <  2:165247/149‑1 (MQ=255)
                                               agagagCTGGCACGCTTCGTTTCTTAGCGTGTAGTCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTTATAACGCGCCAAGATTTAGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAgg                                                                                                      <  1:251576/149‑1 (MQ=255)
                                               agagagCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAgg                                                                                                      >  8:95130/1‑149 (MQ=255)
                                                gagagCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAggc                                                                                                     <  4:100593/149‑1 (MQ=255)
                                                  gagcgcgCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAggcgg                                                                                                   <  8:244060/143‑1 (MQ=255)
                                                  gagCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAggcg                                                                                                    <  7:269463/148‑1 (MQ=255)
                                                     cttgCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCa                                                                                                 >  4:137560/4‑148 (MQ=255)
                                                     cTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCaa                                                                                                >  7:44716/1‑149 (MQ=255)
                                                              gtcgtTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCtcgtcgt                                                                                       >  7:70822/1‑149 (MQ=255)
                                                                 gtTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGa                                                                                    <  8:214795/149‑1 (MQ=255)
                                                                     cTGAGCGTGTAGGCCATGTCACGCAAGACTATATGCCAGAGAAGTCCGCAGCTCTTATAACGCGCCAAGATGTCGACCGAGTCTACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGActc                                                                                 <  4:210020/148‑1 (MQ=255)
                                                                     cTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGActc                                                                                 <  5:221254/148‑1 (MQ=255)
                                                                        aGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTAGTCGACTCTCAc                                                                             >  5:393356/1‑149 (MQ=255)
                                                                         gCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCAcc                                                                            <  6:45590/149‑1 (MQ=255)
                                                                            tgtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCgg                                                                         <  4:12038/149‑1 (MQ=255)
                                                                            tgtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCgg                                                                         <  2:379607/149‑1 (MQ=255)
                                                                            tgtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCgg                                                                         <  7:50697/149‑1 (MQ=255)
                                                                                 gCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTAc                                                                     >  6:26450/1‑148 (MQ=255)
                                                                                   cAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                  >  3:373750/1‑149 (MQ=255)
                                                                                   cAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                  >  3:171446/1‑149 (MQ=255)
                                                                                   cAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                  <  8:9286/149‑1 (MQ=255)
                                                                                    aaGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCATGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                  <  1:317212/148‑1 (MQ=255)
                                                                                    aaGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCCGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCCCCCGGTGACGa                                                                   >  2:360932/1‑147 (MQ=255)
                                                                                    aaGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                  >  2:317212/1‑148 (MQ=255)
                                                                                         aCGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGaaa                                                             <  4:171446/148‑1 (MQ=255)
                                                                                           gCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACAt                                                          >  4:16387/1‑149 (MQ=255)
                                                                                             aaGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTc                                                        >  6:109303/1‑149 (MQ=255)
                                                                                              aGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCg                                                       <  3:16387/149‑1 (MQ=255)
                                                                                               gACGATATGCGAGAGAAGTCCGCAGCTATGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCgg                                                      >  1:210051/1‑149 (MQ=255)
                                                                                                aCGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGt                                                     <  2:320664/149‑1 (MQ=255)
                                                                                                  gATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGtt                                                    >  7:121915/1‑148 (MQ=255)
                                                                                                  gATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTAAGATCGAAACATTCGGtt                                                    >  4:223581/1‑148 (MQ=255)
                                                                                                    tatGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCa                                                 <  8:70822/149‑1 (MQ=255)
                                                                                                      tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAgg                                               >  5:215525/1‑149 (MQ=255)
                                                                                                      tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAg                                                <  8:121915/148‑1 (MQ=255)
                                                                                                      tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAg                                                <  2:160952/148‑1 (MQ=255)
                                                                                                      tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAg                                                <  5:147773/148‑1 (MQ=255)
                                                                                                       gCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGAGGCAATCTCGTCGTCGACTCTCACCCCGTTACGATCGAAACATTCGGTTTCAggg                                              >  2:55400/1‑149 (MQ=255)
                                                                                                        cGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGt                                             >  5:87100/1‑149 (MQ=255)
                                                                                                         gagagaAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGt                                             >  2:196592/1‑148 (MQ=255)
                                                                                                                  ccGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTCCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                   <  6:95418/149‑1 (MQ=255)
                                                                                                                   cGCAGCTCTGATAACGCGCCAAGATGTCGACCGCGTCGACAGGGAGTTGATTGC‑GGTGGCGTCAGCATTCAAAAAAGACGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                   <  6:90021/148‑1 (MQ=255)
                                                                                                                   cGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTg                                  <  8:44716/149‑1 (MQ=255)
                                                                                                                           tGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAgcg                          >  1:201834/1‑149 (MQ=255)
                                                                                                                            gATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                   <  5:379545/139‑1 (MQ=255)
                                                                                                                            gATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                   >  6:379545/1‑139 (MQ=255)
                                                                                                                             aTAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAgc                           <  6:393356/146‑1 (MQ=255)
                                                                                                                              tAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑TGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCa                        <  1:55400/148‑1 (MQ=255)
                                                                                                                              tAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAg                       >  4:156144/1‑149 (MQ=255)
                                                                                                                                aCGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGtt                     >  6:257307/1‑149 (MQ=255)
                                                                                                                                aCGCGACAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTAACCCTGTTTCGATCGAAACAATCGGTTTCATGTTGACGCCATTTGCAAAAGCGCAttt                     >  8:89734/1‑149 (MQ=255)
                                                                                                                                  gcgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTg                    >  8:190962/1‑148 (MQ=255)
                                                                                                                                  gcgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTg                    >  8:231548/1‑148 (MQ=255)
                                                                                                                                   cgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCa                  <  6:87100/149‑1 (MQ=255)
                                                                                                                                   cgcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCa                  <  2:201834/149‑1 (MQ=255)
                                                                                                                                      cAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCACACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGa               >  1:340477/1‑149 (MQ=255)
                                                                                                                                         cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGa                                           >  6:143444/3‑118 (MQ=255)
                                                                                                                                         cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGa                                           <  5:143444/116‑1 (MQ=255)
                                                                                                                                         cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCt            >  2:201595/3‑149 (MQ=255)
                                                                                                                                         cctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCt            >  6:222846/3‑149 (MQ=255)
                                                                                                                                          ctGTCGACTCGGTCGACAGGGAGTTGATTGCGGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAgg                                               >  1:243531/2‑114 (MQ=255)
                                                                                                                                          ctGTCGACTCGGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAgg                                               <  2:243531/112‑1 (MQ=255)
                                                                                                                                               gACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc       <  5:109303/148‑1 (MQ=255)
                                                                                                                                                aCCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc       >  7:209263/1‑147 (MQ=255)
                                                                                                                                                 ccGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc       >  5:154389/1‑146 (MQ=255)
                                                                                                                                                   gAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGCCAGAc  >  6:302721/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGC‑GGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGCCAGAC  >  NC_002947/6152478‑6152773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑